Detection method used for measuring doxercalciferol and impurities contained by doxercalciferol

A detection method and technology for docetaxel, applied in the field of chemical detection, can solve the problems of long relative retention time of the main peak, unfavorable impurities, influence on the therapeutic effect of docetaxel preparations, etc., so as to reduce the relative retention time and improve the efficiency. Effect

Active Publication Date: 2017-05-31
ZHUOHE PHARM GRP CO LTD
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the detection time of this method is longer, and the relative retention time of main peak is longer, and the impurity response value at 274nm wavelength place is lower, makes the impurity co...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method used for measuring doxercalciferol and impurities contained by doxercalciferol
  • Detection method used for measuring doxercalciferol and impurities contained by doxercalciferol
  • Detection method used for measuring doxercalciferol and impurities contained by doxercalciferol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] The present invention will be described in detail below in conjunction with the implementations shown in the drawings, but it should be noted that these implementations are not limitations of the present invention, and those of ordinary skill in the art based on the functions, methods, or structural changes made by these implementations Equivalent transformations or substitutions all fall within the protection scope of the present invention.

[0033] Unless otherwise specified in the description, the components and raw materials in each example of the present invention are of analytical grade. In addition, "g" in each embodiment is the weight unit "gram"; "h" is the time unit "hour"; "ml" is the volume unit "milliliter"; "room temperature" is 23°C. "vol%" is the concentration unit "percentage by volume".

[0034] A method for the separation and determination of calciferol and related substances by high-performance liquid chromatography. The chromatographic column used ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a detection method used for measuring doxercalciferol and impurities contained by doxercalciferol. According to the detection method, high performance liquid chromatography is adopted, and chromatographic conditions are determined as following: globular protein bonded with hydrophilic silicone or octadecyl silane is taken as the stationary phase, a mixed solution composed of an acid solution and an organic solvent is taken as the mobile phase, flow velocity is controlled to be 1.5 to 2.0ml/min, detection wavelength is controlled to be 210nm, column temperature is controlled to be room temperature, sample size is controlled to be 5 to 10<mu>L, sample concentration is controlled to be 3 to 5mg/ml, and the volume ratio of the acid solution to the organic solvent solution in the mixed solution is reduced in a gradient manner with elution progress of high performance liquid chromatography. In the gradient elution process of high performance liquid chromatography, the volume ratio of the acid solution to the organic solvent solution in the mixed solution is reduced in a gradient manner with elution progress of high performance liquid chromatography, so that synchronous separation and detection of doxercalciferol and three impurities contained by doxercalciferol are realized, separation and detection efficiency is increased, and detection time and relative retention time of main peaks are shortened.

Description

technical field [0001] The invention relates to the technical field of chemical detection, in particular to a detection method for measuring calciferol and impurities contained therein. Background technique [0002] Doxercalciferol, also known as 1-A hydroxyvitamin D2, is a vitamin D2 analog that mainly acts on the parathyroid gland, can effectively inhibit the secretion of the whole parathyroid hormone, and increase blood calcium and blood levels to a small extent. Phosphorus concentration, new drugs such as SHPT approved by FDA for patients with osteoporosis, grade 3 to 4 chronic kidney disease. The molecular formula of docecalcidol is: C 28 h 44 o 2 , and its chemical structure is as follows: [0003] [0004] During the synthesis of doxercalciferol, some related impurity substances such as by-products or intermediates or degradation products produced during the production process will be produced. These impurities may affect the purity and quality of the drug due...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/34
CPCG01N30/34
Inventor 王杨马国敏张莹杨芳刘倩米靖宇徐建国
Owner ZHUOHE PHARM GRP CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap