Device and method for selecting eukaryotic cells in a transportation channel by altering the eukaryotic cells by means of electromagnetic radiation
A transport channel, eukaryotic cell technology, applied in the field of animal sperm, can solve problems such as technical feasibility yet to be proved
Inactive Publication Date: 2017-05-31
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Problems solved by technology
To the applicant's knowledge, such a technical solution is not currently in commercial use, and the technical feasibility of a solution of this type remains to be demonstrated
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[0074] figure 1 A first variant embodiment of the device that allows selection of spermatozoa according to their chromosomes, for example their X or Y chromosome, is shown.
[0075] The following detailed description focuses on the selection of spermatozoa with different chromosomal types. However, the invention is not limited to the selection of spermatozoa, but can be applied more generally to the selection of eukaryotic cells which may be of different types, enabling the cataloging of these eukaryotic cells into at least two different classes. More particularly but not exclusively, eukaryotic cells may differ, for example, by the DNA of their nucleus.
[0076] figure 1 The equipment includes:
[0077] - a microfluidic chip 1 comprising main microfluidic transport channels 100,
[0078] - an injection device 2 enabling the injection of a solution S of a sample containing sperm SP to be selected into the main microfluidic transport channel 100,
[0079] - a hydrodynamic ...
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Abstract
The device can be used for the in vitro selection of eukaryotic cells, and in particular sperm cells (SP). It comprises a transportation channel (100), preferably a DE TR transportation channel (100), in which a solution containing said eukaryotic cells can circulate (SP), a first through passage (104) opening into said transportation channel (100), a source of electromagnetic radiation (60), which is coupled to a first end of a first optical fibre (61), the other emission end (61b) of the first optical fibre being inserted into said first through passage (104), without protruding into the transportation channel (100). The device further comprises electronic control means (7), which make it possible to automatically control said source of electromagnetic radiation (60), so as to selectively alter the eukaryotic cells (SP) circulating in the transportation channel (100), by means of the electromagnetic alteration radiation (R) emitted by the source of electromagnetic radiation (60).
Description
technical field [0001] The present invention relates to the field of in vitro selection of eukaryotic cells, in particular but not limited to spermatozoa, especially animal spermatozoa, using electromagnetic radiation. Background technique [0002] Sperm are eukaryotic cells of the haploid type, which generally contain only one sample of each chromosome, such as the X or Y chromosome, whose motility is ensured by flagella. [0003] In the field of animal reproduction, different sex selection solutions have been developed so far, enabling automatic selection and sorting of spermatozoa based on their X or Y chromosome, and which are based on flow cytometry. [0004] In general, all of these technical options for sex selection by flow cytometry are based on the fact that X sperm contain about 4% more DNA than Y sperm. Thus, among these gender-selective technology options: [0005] (a) Sperm are labeled with fluorescent DNA intercalants (commonly referred to as fluorescent dye...
Claims
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IPC IPC(8): G01N15/14C12N5/071
CPCG01N15/1459G01N15/1434G01N15/1484C12N5/0612G01N15/149C12M1/42G01N15/14
Inventor L·里埃吉奥斯E·库尔塔德H·达玛特J·皮瑟Q·瑟门A·崔泽布尔
Owner GENES DIFFUSION