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Castanopsis hystrix tender branch tissue culture and propagation method

A technology of red cone and branch group is applied in the field of plant reproduction, which can solve the problems of low bud proliferation coefficient and slow growth, and achieve the effects of simple operation process, large yield of sub-buds and excellent quality.

Inactive Publication Date: 2017-06-06
唐春艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for tissue culture and propagation of red cone tender shoots with good growth effect and fast propagation for the problems of low bud proliferation coefficient and slow growth in the tissue culture process of red cone

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Selection of explants: Collect semi-lignified, vigorously growing twigs of the same year as explants;

[0028] (2) Treatment of explants: Rinse the explants under tap water for 10 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 15 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0029] (3) Initial bud induction culture: Inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 500 lx, and culture at 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified WPM medium+6-BA 4.0 mg / L+NAA ...

Embodiment 2

[0035] (1) Selection of explants: Collect semi-lignified, vigorously growing twigs of the same year as explants;

[0036] (2) Treatment of explants: Rinse the explants under tap water for 15 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0037] (3) Initial bud induction culture: inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 1000 lx, and culture temperature 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified WPM medium+6-BA 5.0...

Embodiment 3

[0043] (1) Selection of explants: Collect semi-lignified, vigorously growing twigs of the same year as explants;

[0044] (2) Treatment of explants: Rinse the explants under tap water for 15 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0045] (3) Initial bud induction culture: inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 1000 lx, and culture temperature 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 improved WPM medium+6-BA 6.0...

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PUM

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Abstract

The invention discloses a castanopsis hystrix tender branch tissue culture and propagation method which comprises the following procedures of explant selection, explant treatment, initial bud induction culture, multiplication culture, strong bud culture and rooting culture. Underyearling semi-lignified and robustly growing castanopsis hystrix tender branches are collected as explants, the explants are inoculated into an initial bud induction medium after being treated by trimming and sterilization and disinfection treatment to acquire initial buds, then the initial buds are inoculated into a proliferation medium to carry out proliferation culture for 3-4 cycles to form multiple bud clusters, the multiple bud clusters access to a strong bud medium to be cultured, and finally, single buds with height being larger than or equal to 2cm are inserted into a rooting medium to be induced to grow roots. According to the castanopsis hystrix tender branch tissue culture and propagation method, the castanopsis hystrix seedling cycle is shortened, a seedling material is saved, the problems that the amount of materials needed by seedling is more, the cycle is long and the like in the traditional seedling method are overcome, the production cost is greatly lowered, the production efficiency is improved, and the castanopsis hystrix tender branch tissue culture and propagation method has better economic benefit, social benefit and ecological benefit.

Description

technical field [0001] The invention belongs to the technical field of plant propagation and relates to a method for tissue culture propagation of red cone twigs. Background technique [0002] Castanopsis hystrix A .DC belongs to the Fagaceae genus Castanopsis, the adult tree height can reach 30m, diameter at breast height is more than 1m, and it is the dominant tree species in the evergreen broad-leaved forest of subtropical and subtropical vegetation in my country. It is an important native broad-leaved precious timber and high-efficiency multi-purpose ecological public welfare forest tree species in South China. Red cones are naturally distributed in east longitude 95°20′~118°00′ and north latitude 18°30′~25°00′. The main production areas are concentrated in Guangdong, Guangxi, and southern Fujian, and its surrounding areas are distributed in Hainan, southern Yunnan, and southeastern Guizhou. And Hunan, Jiangxi and other provinces in the south. [0003] Red cone has exce...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008Y02P60/40
Inventor 唐春艳陈素云
Owner 唐春艳
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