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A kind of composite microbial agent for preventing and treating rice blast and preparation method thereof

A compound microbial inoculum and rice blast technology, applied in the field of microorganisms, can solve the problems of different levels of antagonistic bacteria, lack of single use, easy to produce drug resistance, etc., and achieve no toxic side effects, high control effect, and best control effect. and the effect of increasing productivity

Active Publication Date: 2018-02-23
湖南格灵科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although live antagonistic bacteria against the rice blast fungus are used as pesticide preparations, it can not only avoid the hidden dangers of chemical pesticides with its safer biological metabolites, but also use the antagonistic correspondence between antagonistic bacteria and pathogenic bacteria to reduce the diversity of rice blast pathogens. , the impact of easy variation, but the ability of the screened antagonistic bacteria is different, and there is still a lack of single use, which is easy to produce drug resistance. At the same time, the prevention effect needs to be further strengthened

Method used

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  • A kind of composite microbial agent for preventing and treating rice blast and preparation method thereof
  • A kind of composite microbial agent for preventing and treating rice blast and preparation method thereof
  • A kind of composite microbial agent for preventing and treating rice blast and preparation method thereof

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Effect test

Embodiment 1

[0038] Embodiment 1: prepare the spore of Actinomycetes CZ1367

[0039] Inoculate actinomycetes CZ1367 on the slant of solid medium in the test tube. The medium formula is: 200g of potatoes, 20g of glucose, 20g of agar, 1000ml of water, pH value 7.4, and culture at 28°C for 7 days to obtain test tube species;

[0040] Prepare liquid seed medium first, the formula of liquid seed medium is yeast extract 4g, glucose 4g, malt extract 10g, agar 20g, water 1000ml, pH value 7.2; put 100ml liquid medium in each conical flask, extinguish at 121℃ Bacteria for 30 minutes, after cooling, insert 1cm 2 The test-tube species was shaken and cultivated at 28°C for 7 days at a rotation speed of 200r / min to obtain seed liquid;

[0041] Prepare a solid fermentation medium first, the formula of the solid fermentation medium is bran 1000g, sterilize at 121°C for 60 minutes, dry after cooling, insert the seed liquid obtained in step 2 with 30% inoculation amount, and add sterile water until the wa...

Embodiment 2

[0042] Embodiment 2: prepare the thalline and spore of Bacillus pumilus WX812

[0043] Inoculate Bacillus pumilus WX812 on the slant of solid medium in the test tube. The medium formula is: 10g NaCl, 10g peptone, 5g yeast powder, 20g agar, 1000ml water, pH 7.2, and culture at 30°C for 1 day to obtain the test tube species;

[0044] Prepare liquid seed medium first, the formula of liquid seed medium is NaCl 10g, peptone 10g, yeast powder 5g, water 1000ml, pH7.2; put 100ml liquid medium in each Erlenmeyer flask, sterilize at 121°C for 30 minutes, Insert 1cm after cooling 2 The test-tube seeds were shaken and cultured at 30°C at a rotation speed of 200r / min for 6-8h to obtain seed liquid;

[0045] Prepare the liquid fermentation medium first, the formula of the liquid fermentation medium is soybean powder 2%, sucrose 1%, soluble starch 0.5%, peptone 0.2%, NaCl 0.2%, CaCO 3 0.1%, MgSO 4 ·7H 2 O 0.05%, KH 2 PO 4 0.05%, pH7.0; 121 DEG C of sterilizing for 30 minutes, after c...

Embodiment 3

[0046] Embodiment 3: plate antagonistic antibacterial test

[0047] Treatment 1: CZ1367+WX812

[0048] Using the plate confrontation method, inoculate the activated Actinomycetes and Bacillus pumilus on the PDA medium plate with a diameter of 90 mm, 25 mm from the center of the petri dish, and then inoculate the blast fungus at the center of the petri dish. Seal the petri dish with a parafilm, and incubate it upside down in a fungal incubator at 28°C for more than 7 days. Repeat each treatment 3 times, and use no antagonistic bacteria as the CK control, and observe the experimental results after 7 days. The fungal colony diameter was measured and the fungal mycelial growth inhibition rate was calculated.

[0049] Process 2: CZ1367

[0050] Using the plate confrontation method, the activated actinomycete CZ1367 was inoculated on a PDA medium plate with a diameter of 90 mm at a position 25 mm away from the center of the petri dish, and then the blast pathogen was inoculated at...

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Abstract

The invention relates to the technical field of microorganism, and discloses a compound microorganism bacterium agent capable of preventing and controlling rice-blast and a preparation method thereof. The compound microorganism bacteria agent is prepared from a spore of actinomyces CZ1367 of which the preservation number is CCTCC No: M 2016277 and the thallus and germ of bacillus pumilus WX812 of which the preservation number is CCTCC No: M 2016276. The actinomyces and bacillus pumilus coordinate to synergistically prevent and control rice-blast bacteria, not only can make up deficiency on mutually prevention and control effects, but also can avoid using chemical pesticide, and enable to be environment-friendly, and free from toxic and side effects, and drug resistance of the pathogenic bacteria is more difficult to produce by comparing with single rice-blast antagonistic bacteria.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a compound microbial agent for preventing and treating rice blast, a preparation method and application thereof. Background technique [0002] Rice blast (Blast Fungus), also known as rice fever, is one of the most serious rice diseases in the world. The disease can cause harm to rice all year round, and its harm spreads all over the various parts of rice, including seedling rice blast, leaf blast, leaf pillow blast, node rice blast, ear neck blast, branch blast and grain blast. Generally, rice production is reduced by 10% to 20%. Taking my country as an example, since the 1990s, the annual occurrence area of ​​the disease has been 3.8 million hectares 2 Above, the annual loss of rice reaches hundreds of millions of kilograms. [0003] At present, chemical pesticides are one of the main measures to prevent and control rice blast. However, large-scale and long-term use o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/04C12R1/07
CPCA01N63/10C12N1/20
Inventor 钟积东谭志奇周艳
Owner 湖南格灵科技有限公司