Application of Second Harmonic Imaging Combined with Fluorescence Imaging for Locating Transdermal Absorption of Substances

A substance and transdermal technology, applied in the field of biomedicine, can solve the problems of inability to dynamically observe the position and retention of drugs entering the skin, and inability to mark the position information of macromolecular drugs, and achieve the effect of simple operation and clear characterization.

Active Publication Date: 2020-06-12
EXPERIMENTAL RES CENT CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even in the field of medical research, the existing tissue localization observation methods such as pathological section method can clarify the pathological information of the sample, but cannot mark the location information of macromolecular drugs on the section
As a traditional instrument for studying trans-absorption, the transdermal absorption instrument cannot dynamically observe the position and retention of the drug entering the skin

Method used

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  • Application of Second Harmonic Imaging Combined with Fluorescence Imaging for Locating Transdermal Absorption of Substances
  • Application of Second Harmonic Imaging Combined with Fluorescence Imaging for Locating Transdermal Absorption of Substances
  • Application of Second Harmonic Imaging Combined with Fluorescence Imaging for Locating Transdermal Absorption of Substances

Examples

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Embodiment 1

[0027] Example 1. Detection of Human Collagen Transdermal Absorption by Second Harmonic Combined with Two-Photon Fluorescence Imaging

[0028] 1. Fluorescently labeled R-hc

[0029] Accurately weigh 100 mg of human collagen R-hc dry powder, add 10 mL of phosphate buffer to fully dissolve it, and use 1mol / L NaHCO 3 The solution was adjusted to pH 8.5±0.5. iFluor TM Add 1ml of anhydrous DMSO to 350 (1mg) to obtain a 1mg / mL dye solution. While stirring with a magnetic stirrer, add the fluorescent dye solution drop by drop to the protein solution to avoid sticking the fluorescein to the wall of the flask (the addition should be completed within about 20 minutes). After the addition, continue to stir in the dark for about 12 hours. During the binding period, the protein solution should be kept at about 4°C, so the beaker and stirrer should be moved together into a 4°C refrigerator. Take out the fluorescently labeled protein and put it into a dialysis bag, put it into a large be...

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Abstract

The invention discloses application of second harmonic imaging and fluorescent imaging in substance positioning of transdermal absorption, and provides a method for detecting the transdermal absorption condition of a to-be-detected substance. The method comprises the step of observing the transdermal absorption process and / or gathering position in a skin tissue of a fluorescence labeled to-be-detected substance by taking an SHG signal generated from collagen in a skin corium layer as a structural internal reference of a skin tissue. Experiments prove that the SHG signal of collagen in the corium layer is utilized as a structural internal reference of the skin tissue to observe the transdermal absorption process and gathering position of fluorescence labeled collagen and observe whether the fluorescence signal is superposed with the SHG signal or not. The second harmonic imaging and fluorescent imaging has clear representation, can be used for directly and correctly reflecting the transdermal absorption approach and acting parts of collagen substances, is simple and convenient to operate, and is quick and accurate. A novel detection method is provided for activity detection of trandermal absorption medicines and cosmetic products.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to the application of second harmonic imaging combined with fluorescence imaging in transdermal absorption of locating substances. Background technique [0002] There are three main pathways for substances to penetrate the skin: the stratum corneum, hair follicles and sweat ducts. Among them, the latter two pathways are often called "bypasses", because the skin appendages account for less than 1% of the entire skin surface area, so in most cases they do not become the main absorption pathways, but macromolecular substances and ions Type substances have difficulty passing through the lipid-rich stratum corneum and may enter the skin through these routes. [0003] For the detection of transdermally absorbed substances, there are mainly in vivo methods and in vitro methods. In vivo methods include chemical determination (checking the amount of substances absorbed into the blood), tissue inspe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 王毅孙娅楠赵静
Owner EXPERIMENTAL RES CENT CHINA ACAD OF CHINESE MEDICAL SCI
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