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A high-efficiency inducing differentiation agent and inducing differentiation medium for adipose stem cells

A technology of fat stem cells and smooth muscle cells, applied in the field of stem cells, can solve problems such as system instability

Active Publication Date: 2020-10-09
PORTON BIOLOGICS LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Yang Jin from West China Hospital established a system for inducing adipose-derived stem cells to differentiate into bladder smooth muscle cells in vitro using TGF-β and ascorbic acid as inducers, but the system was unstable and would differentiate into chondrocytes in some cases

Method used

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  • A high-efficiency inducing differentiation agent and inducing differentiation medium for adipose stem cells
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  • A high-efficiency inducing differentiation agent and inducing differentiation medium for adipose stem cells

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Embodiment Construction

[0023] The substantive content of the present invention will be specifically introduced below in conjunction with the embodiments. Due to space reasons, the description of the experimental process cannot be done in great detail. All the parts not described in detail in the experiment are routine operations well known to those skilled in the art.

[0024] 1. Experimental materials

[0025] The plasma for preparing plasma exosomes comes from healthy adults, and healthy adults refer to healthy adults who have normal physical examination and exclude other chronic diseases. The source of fat was collected from healthy women aged 20-40 during liposuction, and the history of endocrine and chronic diseases was excluded before the operation. Informed consent was signed before the operation.

[0026] 2. Experimental method

[0027] 1. Preparation of plasma exosomes

[0028] Collect 5ml of venous blood from a healthy adult with an EDTA anticoagulant tube, let it stand for 10 minutes, ...

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Abstract

The invention discloses an efficient differentiation induction agent of adipose-derived stem cells and a differentiation induction culture medium. The invention belongs to the field of stem cells, relates to stem cell differentiation induction and in particular relates to the efficient differentiation induction agent of the adipose-derived stem cells and the differentiation induction culture medium; the adipose-derived stem cells are efficiently induced and are directionally differentiated into bladder smooth muscle cells; the differentiation induction agent and the differentiation induction culture medium contain an effective amount of blood serum exosomes. The differentiation induction agent and the differentiation induction culture medium, provided by the invention, prove that the blood serum exosomes can be used for effectively inducing the adipose-derived stem cells to directionally differentiating the adipose-derived stem cells into the bladder smooth muscle cells and can be used for preparing the efficient differentiation induction agent of the adipose-derived stem cells and the differentiation induction culture medium; the adipose-derived stem cells can be efficiently induced and are directionally differentiated into the bladder smooth muscle cells.

Description

technical field [0001] The invention belongs to the field of stem cells, and relates to stem cell induction and differentiation, in particular to a high-efficiency induction differentiation agent and induction differentiation medium for adipose stem cells, which can efficiently induce the directional differentiation of fat stem cells into bladder smooth muscle cells. Background technique [0002] Congenital malformations and acquired structure or function loss of urinary system organs are common problems in the field of clinical urology. At present, the repair and reconstruction of urinary system still relies on the use of gastrointestinal segments to replace the defective bladder and other organs. Tissue engineering technology provides a new direction for the repair and reconstruction of the urinary system, but the mature bladder smooth muscle cells as seed cells for tissue engineering have shortcomings such as insufficient source, easy aging, and lack of self-renewal abilit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0661C12N2506/1384
Inventor 王莉徐梦龙
Owner PORTON BIOLOGICS LTD
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