Scale production method for high-content astaxanthin haematococcus pluvialis

A production method, astaxanthin technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of slow growth, growth inhibition, pollution, etc.

Inactive Publication Date: 2017-06-30
烟台布鲁拜尔生物制药有限公司
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Problems solved by technology

Most of the algae strains used in laboratory research and large-scale cultivation in the past were wild strains isolated from nature, and there are many defects: they are easily polluted by other microalgae, amoebas,

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  • Scale production method for high-content astaxanthin haematococcus pluvialis
  • Scale production method for high-content astaxanthin haematococcus pluvialis
  • Scale production method for high-content astaxanthin haematococcus pluvialis

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Embodiment Construction

[0054] Mutation Breeding and Mutant Screening of Haematococcus Pluvialls

[0055] Although Haematococcus pluvialis is known as the biological resource with the highest content of natural astaxanthin, its content still needs to be increased to be suitable for large-scale production. Most of the algae strains used in laboratory research and large-scale cultivation in the past were wild strains isolated from nature, and there are many defects: they are easily polluted by other microalgae, amoebas, protozoa, bacteria, etc. during the cultivation process; they are only suitable for low temperature cultivation, 28 ℃ The above growth is inhibited; the growth is relatively slow, and the culture period is long. Therefore, screening Haematococcus pluvialis strains with wide temperature adaptation range, fast growth rate and high astaxanthin content has always been the direction of efforts in this field, and it is also the first problem to be solved in this project. This topic adopts th...

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Abstract

The invention discloses a scale production method for high-content astaxanthin haematococcus pluvialis. The scale production method includes algae species culture, species bottle culture, closed tubular photobioreactor culture, indoor pond photobioreactor culture and outdoor pond reactor astaxanthin accumulation culture. Algae species rapidly grow and breed under an optimized growth condition in a laboratory and are accumulated to reach a certain cell concentration and then inoculated to a closed tubular photobioreactor by a sterile tube for secondary enlarged culture, the algae species are inoculated to an indoor pond photobioreactor for tertiary growth culture to reach the highest algae cell quantity after haematococcus pluvialis grows to a certain density, cultured haematococcus pluvialis green cells are transferred into the outdoor pond photobioreactor and accumulate a large quantity of astaxanthin under stressed conditions, and the scale production process of the haematococcus pluvialis is finally finished. Compared with the prior art, the scale production method has the advantage that the astaxanthin content exceeds international level reported at present and reaches 7.2% or more.

Description

technical field [0001] The invention relates to the technical field of biological culture, in particular to a method for cultivating Haematococcus with high content of astaxanthin. Background technique [0002] Astaxanthin has a strong ability to resist oxidation and capture free radicals, and can effectively quench a large amount of waste such as singlet oxygen and free radicals produced in muscles during strenuous activities. The chemical properties of free radicals are very active, and they are very easy to participate in redox reactions, which can trigger chain reactions and lead to lipid peroxidation on biological membranes, thereby destroying the structure and function of membranes; they can cause protein denaturation and cross-linking, making Inactivation of enzymes and hormones; lowering the body's immunity, destroying the structure of nucleic acids and causing metabolic abnormalities, etc. The astaxanthin molecule has a long conjugated double bond, a hydroxyl group...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N15/01C12P23/00C12R1/89
CPCC12N1/12C12N15/01C12P23/00
Inventor 朱展董浩曹喜梅杨兰苹高嵩
Owner 烟台布鲁拜尔生物制药有限公司
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