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Application of microRNA (Ribonucleic Acid) molecular expression profile for predicting non-small cell lung cancer brain metastasis risk

A non-small cell lung cancer, microRNA-210 technology, applied in the direction of DNA/RNA fragment, recombinant DNA technology, microorganism determination/inspection, etc., can solve the problems of lack and small sample size

Pending Publication Date: 2017-07-18
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, only 3 studies have involved the difference of microRNA expression in NSCLC brain metastases, however, the sample size of these studies is too small, and lack of complete mechanism study
So far, no more mature molecular markers can or are expected to be applied clinically to predict high-risk subgroups of brain metastases from non-small cell lung cancer

Method used

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  • Application of microRNA (Ribonucleic Acid) molecular expression profile for predicting non-small cell lung cancer brain metastasis risk
  • Application of microRNA (Ribonucleic Acid) molecular expression profile for predicting non-small cell lung cancer brain metastasis risk
  • Application of microRNA (Ribonucleic Acid) molecular expression profile for predicting non-small cell lung cancer brain metastasis risk

Examples

Experimental program
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Embodiment

[0037] Example, RNA extraction, quality detection, chip hybridization and data processing

[0038] 1. Dewaxing of limonene to treat paraffin-embedded tissues

[0039] Pretreatment of paraffin-embedded tissue samples: If the paraffin-embedded tissue has been sliced, you can choose about ten slices with a thickness not greater than 50 μm, try to remove the surrounding paraffin, and place it in a 1.5mL centrifuge tube.

[0040] If the paraffin-embedded tissue is a whole piece, the tissue can be gently scraped off with a sharp blade and placed in a 1.5mL centrifuge tube, trying to avoid scraping off the paraffin. The amount of scraped tissue was controlled within 100mg.

[0041] Limonene Dewaxing Treatment:

[0042] Add 1ml of limonene (Limonen) to the centrifuge tube, shake and mix at 55°C for 5min. Centrifuge at 13,200 rpm for 2 min at room temperature and discard the supernatant. This step was performed 3 times in total.

[0043] Add 1ml of absolute ethanol to the centrifu...

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Abstract

The invention relates to application of a microRNA (Ribonucleic Acid) molecular expression profile for predicting non-small cell lung cancer brain metastasis risk, and in particular to application of one or more primers and / or probes for specifically detecting the microRNA molecular expression profile in preparing reagents for predicting the non-small cell lung cancer brain metastasis risk. The microRNA molecular expression profile comprises one or more of microRNA-193a-5p, microRNA-210, microRNA-214, microRNA-423-3p, microRNA-423-5p and microRNA-4270.

Description

technical field [0001] The present invention relates to the use of microRNA molecules as molecular markers in the prognosis of non-small cell lung cancer. Specifically, the present invention relates to the use of microRNA molecules as molecular markers for predicting the risk of brain metastasis of non-small cell lung cancer, wherein the microRNA molecules are selected from One or more of microRNA-193a-5p, microRNA-210, microRNA-214, microRNA-423-3p, microRNA-423-5p, and microRNA-4270. Background technique [0002] The use of molecular biology techniques can effectively determine the risk of brain metastasis in patients with malignant tumors through molecular level detection, and then provide convenience for individualized treatment of these diseases. [0003] Worldwide, lung cancer is the leading cause of cancer death. Non-small cell lung cancer accounts for about 80% of all lung cancers, and about 25% of non-small cell lung cancer patients will develop brain metastases. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 詹启敏王绿化宋咏梅毕楠孙国贵
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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