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Method for preparing lucid ganoderma culture medium and lucid ganoderma mycelia

A technology of Ganoderma lucidum mycelium and culture medium, applied in the field of preparation of Ganoderma lucidum culture medium and Ganoderma lucidum mycelium, can solve the problems of easy degradation of the culture medium, insufficient activity, poor resistance to stress, etc. The effect of enhancing the activity and inoculation rate of bacteria and improving the yield of Ganoderma lucidum

Inactive Publication Date: 2017-07-21
井冈山市益生缘灵芝生态园有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the cultivation materials of Ganoderma lucidum are mainly composed of sawdust, sawdust, gypsum powder, sugar, and water. The medium of this formula is prone to degeneration during the cultivation process, and the mycelium growth is weak, the vitality is not strong, and the stress resistance is poor. , serious pest damage
[0004] It can be seen from this that, based on the deficiencies in the prior art, can a kind of Ganoderma lucidum culture medium with improved raw material formula be provided, and the preparation method of Ganoderma lucidum mycelium based on the medium, effectively solve the problem of large-scale production of Ganoderma lucidum or even industrialized cultivation? The problems of reduced stress resistance, insufficient activity, poor vitality, and low yield of strains that have emerged have become technical problems to be solved urgently by those skilled in the art

Method used

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  • Method for preparing lucid ganoderma culture medium and lucid ganoderma mycelia
  • Method for preparing lucid ganoderma culture medium and lucid ganoderma mycelia

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1: A Ganoderma lucidum culture medium, including the following raw material components in parts by weight: 50 parts of wood chips, 40 parts of wheat bran, 0.1 parts of gypsum powder, 10 parts of sugar and 0.0001 parts of vitamin B2.

[0030] A preparation method of Ganoderma lucidum mycelium includes the following steps:

[0031] Step 1: Preparation of Ganoderma lucidum culture medium;

[0032] Step 2: Sterilization: Take the prepared culture medium and place it in the culture bag for sterilization;

[0033] Step 3: Inoculation: Inoculate Ganoderma lucidum secondary strains on the culture medium under aseptic conditions;

[0034] Step 4: Cultivation: Take the inoculated Ganoderma lucidum tertiary strain and place it under the conditions of temperature 25°C and humidity 65% ​​to obtain Ganoderma lucidum mycelium.

[0035] The Ganoderma lucidum culture medium is the aforementioned Ganoderma lucidum culture medium.

Embodiment 2

[0036] Example 2: A Ganoderma lucidum culture medium, comprising the following raw material components in parts by weight: 60 parts of wood chips, 35 parts of wheat bran, 0.5 parts of gypsum powder, 5 parts of sugar and 0.0002 parts of vitamin B2.

[0037] A preparation method of Ganoderma lucidum mycelium includes the following steps:

[0038] Step 1: Preparation of Ganoderma lucidum culture medium;

[0039] Step 2: Sterilization: Take the prepared culture medium and place it in the culture bag for sterilization;

[0040] Step 3: Inoculation: Inoculate Ganoderma lucidum secondary strains on the culture medium under aseptic conditions;

[0041] Step 4: Cultivation: Take the inoculated Ganoderma lucidum tertiary strains and place them at a temperature of 28°C and a humidity of 62% to cultivate Ganoderma lucidum mycelium.

[0042] The Ganoderma lucidum culture medium is the aforementioned Ganoderma lucidum culture medium.

Embodiment 3

[0043] Example 3: A Ganoderma lucidum culture medium, including the following raw material components in parts by weight: 70 parts of wood chips, 30 parts of wheat bran, 1 part of gypsum powder, 4 parts of sugar, 0.00022 parts of vitamin B2, 0.01 parts Of magnesium sulfate, 0.5 part of superphosphate and 0.01 part of potassium iodide.

[0044] A preparation method of Ganoderma lucidum mycelium includes the following steps:

[0045] Step 1: Prepare the Ganoderma lucidum culture medium: Spread the sawdust evenly and evenly sprinkle with wheat bran and gypsum powder to obtain the sawdust mixture, prepare the sugar into sugar water, and dissolve the crushed vitamin B2, magnesium sulfate, calcium superphosphate and potassium iodide into the sugar water. Sprinkle the sugar water in which vitamin B2 is dissolved on the surface of the sawdust mixture evenly, and roll the sawdust mixture while sprinkling, so that the raw material components are fully mixed, so that the final water content o...

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Abstract

The invention relates to the technical field of edible mushroom cultivation, in particular to a method for preparing a lucid ganoderma culture medium and lucid ganoderma mycelia. The lucid ganoderma culture medium comprises the following raw material components in parts by weight: 50-150 parts of bits of wood, 10-40 parts of wheat bran, 0.1-10 parts of gypsum powder, 0.1-10 parts of sugar and 0.0001-0.001 part of vitamin B2. The method for preparing the lucid ganoderma culture medium and the lucid ganoderma mycelia, which is provided by the invention, is scientific and reasonable in raw material formula matching, the lucid ganoderma mycelia obtained through inoculation of the culture medium are relatively high in activity and relatively high in inoculation rate, and furthermore the planting yield of the lucid ganoderma is increased. Meanwhile, the method for preparing the lucid ganoderma culture medium and the lucid ganoderma mycelia, which is provided by the invention, has the advantages of being high in efficiency, low in cost, simple to operate and good in practicability.

Description

Technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a method for preparing a Ganoderma lucidum culture medium and a Ganoderma lucidum mycelium. Background technique [0002] Ganoderma is the fruiting body of the fungus Ganoderma of the Polyporaceae family. It has the effects of replenishing qi and soothe the nerves, relieving cough and asthma, prolonging life. Ganoderma has been used in my country for more than 2,000 years as a medicinal product. Modern medical research has found that Ganoderma lucidum is very rich in nutrients. The effective ingredients can include Ganoderma lucidum triterpenes, Ganoderma lucidum polysaccharides, Ganoderma lucidum polypeptides, 16 amino acids (including seven essential amino acids), steroids, mannitol, and coumarin Glycosides, alkaloids, organic acids (mainly containing fumaric acid), and trace elements Ge, P, Fe, Ca, Mn, Zn, etc. It has a two-way regulation effect on the human bo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 章晋武刘铖韩旭邱发金黄龙
Owner 井冈山市益生缘灵芝生态园有限公司
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