Method for rapidly restoring lily distant hybrid fertility
A distant hybrid, rapid recovery technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of complicated procedures for importing bulbs, pests and diseases, and poor quality.
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Embodiment 1
[0015] 1) Cultivation of distant lily: choose a healthy and free species of distant lily with a bulb circumference greater than 12 cm, and carry out lily cultivation and management according to conventional methods;
[0016] 2) Treatment of lily buds: Select bulbs of distant lilies that can bloom, and cultivate them by conventional methods. When the buds appear, use cotton balls dipped in 4-10μM concentration of amisulfame solution to cover the buds on the tip of the stem, and use 4-10μM The flower buds of the distant hybrid lily were treated with amisulfameline for 2-3 days, and then washed with water for 5 times.
[0017] 3) Fertility recovery identification: The sign of fertility recovery of distant hybrids of lilies is that their pollen is fertile, and untreated pollen is sterile, and pollen germination cannot be seen under a microscope. The fertile pollen grains obtained from the treatment are gigantic, and the pollen can be seen to germinate and form pollen tubes under a...
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