Xylariaceae and culturing method thereof

A cultivation method, the technology of Pyrethromyces, applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve the problems of cultivating fruiting bodies that have not yet obtained sexual spores, and achieve stable production in large batches, strong antioxidant capacity, and good The effect of economic and social benefits

Active Publication Date: 2017-07-25
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

C. nigriscens is a delicious edible fungus when it is young. The large-scale artificial cultivation of C. nigriscens sclerotium and fruiting bodies has not been successful, and further exploration of cultivation conditions is needed.
The Laboratory of Applied Fungi, Scho

Method used

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  • Xylariaceae and culturing method thereof
  • Xylariaceae and culturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Production of mother species

[0041] 1.1 Isolation of strains

[0042] The comprehensive PDA (20wt% of potato, 2wt% of glucose, 2wt% of agar, 0.3wt% of potassium dihydrogen phosphate, 0.15wt% of magnesium sulfate, vitamin B1 10ppm, the balance is water) is divided into test tubes, at 0.11MPa atmospheric pressure, 121 ℃ High temperature and high pressure damp heat sterilization for 30 minutes, take out and cool and place on an inclined plane. CCTCC NO: M2015746 The fruiting body of C. tertii is wiped with 75% alcohol on the surface under aseptic conditions, then torn apart, and the internal bacterial tissue of 0.2-0.5mm × 0.2-0.5mm is connected to the comprehensive PDA slope. Place it in an incubator at 25°C for constant temperature and dark cultivation. After the mycelia cover the slope, the transfer can be carried out. The time for the mycelium to grow is about 10-15 days.

[0043] 1.2 Purified strains

[0044] Red Bengal culture medium (peptone (peptone) 0.5wt...

Embodiment 2

[0062] Example 2 Determination of antioxidant activity

[0063] 6.1 Preparation of medium PDA solid medium contains 200g potato, 20g glucose, 20g agar, 1000mL water; PDA liquid medium contains 200g potato, 20g glucose, 1000mL water.

[0064] 6.2 Preparation of fermentation broth and mycelia extract. Transfer the strain of C. tertii CCTCC NO: M2015746 into a slant test tube, place it in a constant temperature incubator at 28°C for 5-7 days, and connect 5 pieces after the tube is full (0.5cm×0.5cm) into a 250mL Erlenmeyer flask containing 100mL of medium, placed in a constant temperature shaker at 25°C, at 110r·min -1 Vibrating culture, after the fermentation broth is clarified, take it out, filter with a filter cloth (100 mesh) to obtain the fermentation broth and mycelia respectively, and set aside.

[0065] The mycelium was blotted dry with filter paper and dried at 60°C to constant weight. After grinding with liquid nitrogen, 1.5 g was extracted with distilled water and abs...

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Abstract

The invention discloses Xylariaceae and a culturing method of the Xylariaceae. The Xylariaceae is Xylariaceae sp.HMGIM-130277, and is assigned with the accession number of CCTCC M2015746; the artificial culturing method comprises the following steps: preparing a mother strain, preparing a producing strain and carrying out fruiting management. The Xylariaceae sp.HMGIM-130277 provided by the invention is collected from dead broadleaf wood in the Rainforest Valley of Hainan Jianfengling National Natural Reserve, the invention further provides a corresponding artificial cultivating method, the large-scale stable production is facilitated, and good economic benefit and social benefit are obtained.

Description

technical field [0001] The invention belongs to the field of fungi belonging to the genus Anthracera, and in particular relates to an anthracnose fungi and a cultivation method thereof. Background technique [0002] At present, the edible fungus industry is developing rapidly. The annual output of edible fungi in my country has reached more than 20 million tons, accounting for more than 75% of the world, and the number of employees exceeds 20 million. The fifth place after that surpassed fruit, tea and sericulture. [0003] Today, with the vigorous development of the edible fungus industry, more and more rare edible fungus varieties have gradually entered people's field of vision, and many original rare varieties have gradually been domesticated, such as bamboo fungus, tea mushroom, and lizhi mushroom. However, there are also a large number of wild edible and medicinal fungi that have not been studied because they have not been recognized by humans. According to research, t...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01H15/00A01G1/04C12R1/645
CPCA01H15/00A01G18/00C12N1/14C12N1/145C12R2001/645
Inventor 胡惠萍黄龙花刘远超莫伟鹏吴丽霞
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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