Tea polyphenol composition and application thereof in preparing anti-streptococcus-suis medicines
A technology of Streptococcus suis and its composition, which is applied in the field of tea polyphenols composition and the preparation of anti-Streptococcus suis drugs, can solve the problems of severe drug resistance of bacterial pathogens, achieve strong antioxidant capacity and eliminate odor Effect
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Embodiment 1
[0019] A tea polyphenol composition is composed of epigallocatechin gallate (EGCG), gallocatechin (EGC), epicatechin gallate (ECG), gallocatechin gallate ( GCG) and catechin (EC) were mixed according to the mass ratio of 60.39:18.18:14.55:5.16:1.72 and used in the following examples.
Embodiment 2
[0021] Determination of minimum inhibitory concentration and minimum bactericidal concentration of tea polyphenols composition against Streptococcus suis:
[0022] 1. Materials
[0023] Mueller-Hinton (MH) broth (purchased from BD Company) was used in drug susceptibility experiments to detect the antibacterial efficacy of drugs.
[0024] 2. Test method
[0025] Pick the purified Streptococcus suis type 2 SC19 colony with a sterile inoculation loop, fully disperse it in the MH liquid medium, and prepare the standard bacteria suspension with a concentration of 0.5 McFarland turbidity by McFarland turbidimetry;
[0026] Add tea polyphenols composition solutions of different concentrations to a sterile 96-well polystyrene plate after doubling dilution with MH liquid medium, and add tea polyphenols composition solution to the 1st to 10th wells, each well 50 μl, 50 μl of MH liquid medium was added to the 11th well as a growth control, and only 100 μl of MH liquid medium was added ...
Embodiment 3
[0034] Inhibitory Effect of Tea Polyphenol Composition on Growth Curve of Streptococcus Suis Type 2
[0035] Pick the purified Streptococcus suis type 2 SC19 colony with a sterile inoculation loop, fully disperse it in MH liquid medium, and prepare the standard bacteria suspension with a concentration of 0.5 McFarland turbidimetry by McFarland turbidimetric method.
[0036] The tea polyphenol composition solutions with different concentrations were added to sterile bacterial bottles after doubling dilution with the MH liquid medium, and the growth control only had the MH liquid medium without the tea polyphenol composition solution.
[0037] Inoculate the same amount of 0.5 McFarland turbidimetric standard bacteria suspension into the above-mentioned bacterial bottle, place it on a 37-degree shaker for shaking culture, take the bacterial solution and spread it on an MH plate every two hours to count the viable bacteria, and the result is expressed in CFU / ml express.
[0038] ...
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