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pH-sensitive large-inner-diameter multi-walled carbon nanotube dual drug-loading system and preparation method

A technology of multi-walled carbon nanotubes and double drug loading, which can be used in pharmaceutical formulations, medical preparations without active ingredients, and medical preparations containing active ingredients, etc. Fast and other problems, to achieve the effect of improving the overall drug load, high internal capacity, and large external surface area

Active Publication Date: 2020-04-24
STOMATOLOGICAL HOSPITAL TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are two problems in the application of commonly used chemotherapeutic drugs in clinical practice: one is poor recognition of tumor cells, high toxicity and side effects, while killing tumor cells, it will also damage some normal tissue cells; Fast, low bioavailability, requires multiple injections of large doses to maintain the drug concentration inside the tumor cells to reach the therapeutic level

Method used

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  • pH-sensitive large-inner-diameter multi-walled carbon nanotube dual drug-loading system and preparation method
  • pH-sensitive large-inner-diameter multi-walled carbon nanotube dual drug-loading system and preparation method
  • pH-sensitive large-inner-diameter multi-walled carbon nanotube dual drug-loading system and preparation method

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Embodiment 1

[0029] A preparation method of a pH-sensitive large-inner-diameter multi-walled carbon nanotube dual drug-carrying system, comprising the following steps:

[0030] (1) Take 300 mg of large inner diameter multi-walled carbon nanotubes (abbreviated as LID-MWCNTs, see the electron microscope photo of figure 1 ), put it into a crucible, and heat it in the air environment in the calcining furnace. The heating process is from room temperature to 400 °C at 30 °C min-1, kept for 3 hours, and cooled to room temperature;

[0031] (2) Immerse the LID-MWCNTs obtained in step (1) in 6 mol L -1 Stir in the aqueous hydrochloric acid solution for 6 h, discard the supernatant, wash with ultrapure water and centrifuge until the pH reaches neutral, and vacuum dry at 40°C;

[0032] (3) Put the LID-MWCNTs obtained in step (2) into a mixed acid made of nitric acid and sulfuric acid with a volume ratio of 1:3, and stir in an oil bath at 100° C. for 30 min; neutralize with a saturated aqueous NaOH s...

Embodiment 2

[0041] A preparation method of a pH-sensitive large-inner-diameter multi-walled carbon nanotube dual drug-carrying system, comprising the following steps:

[0042] (1) Take 200 mg of large inner diameter multi-walled carbon nanotubes (abbreviated as LID-MWCNTs) into a crucible, and heat it in an air environment in a calcining furnace. 5h, cooled to room temperature;

[0043] (2) Immerse the LID-MWCNTs obtained in step (1) in 3 mol L -1 Stir in aqueous hydrochloric acid solution for 12 h, discard the supernatant, wash with ultrapure water and centrifuge until pH reaches neutrality, and vacuum dry at 40°C;

[0044] (3) Put the LID-MWCNTs obtained in step (2) into a mixed acid of nitric acid and sulfuric acid with a volume ratio of 1:3, and stir in an oil bath at 100° C. for 25 min; neutralize with a saturated aqueous NaOH solution to a pH of Neutral, get suspension 1, put suspension 1 into a dialysis bag with a molecular weight cut-off of 8000 and conduct dialysis in ultrapure...

Embodiment 3

[0053] A preparation method of a pH-sensitive large-inner-diameter multi-walled carbon nanotube dual drug-carrying system, comprising the following steps:

[0054] (1) Take 400 mg of large inner diameter multi-walled carbon nanotubes (abbreviated as LID-MWCNTs) into a crucible, and heat it in an air environment in a calcining furnace. 1h, cooled to room temperature;

[0055] (2) Immerse the LID-MWCNTs obtained in step (1) in 4 mol L -1 Stir in aqueous hydrochloric acid solution for 8h, discard the supernatant, wash with ultrapure water and centrifuge until pH reaches neutrality, and vacuum dry at 40°C;

[0056] (3) Put the LID-MWCNTs obtained in step (2) into a mixed acid made of nitric acid and sulfuric acid with a volume ratio of 1:3, and stir in an oil bath at 100° C. for 35 min; neutralize with a saturated aqueous NaOH solution to a pH of Neutral, get suspension 1, put suspension 1 into a dialysis bag with a molecular weight cut-off of 14,000 and conduct dialysis in ultr...

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Abstract

The invention discloses a pH-sensitive large-inner-diameter multiwalled carbon nanotube dual drug loading system (CDDP@O-LID-MWCNTs-PEG-FA-DOX for short), and a preparation method thereof. The preparation method comprises following steps: 1, heating of large-inner-diameter multiwalled carbon nanotube; 2, acid treatment; 3, oxidation; 4, filtering, drying, and obtaining of an obtained solid substance; 5, preparing of a cis-platinum-containing suspending liquid; 6, preparing of CDDP@O-LID-MWCNTs-PEG; 7, preparing of CDDP@O-LID-MWCNTs-PEG-FA; and 8, preparing of the CDDP@O-LID-MWCNTs-PEG-FA-DOX. The CDDP@O-LID-MWCNTs-PEG-FA-DOX is large in internal capacity and the external surface area, so that total drug loading capacity is increased; a polyethylene glycol-folic acid-doxorubicin tertiary structure blocking structure is formed on the surface of carbon nanotube, so that pH sensitive release of cis-platinum and doxorubicin is realized.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a pH-sensitive large-inner-diameter multi-walled carbon nanotube double drug-carrying system and a preparation method. Background technique [0002] For many malignant tumors, the anatomical structure and radiosensitivity of their location limit the resection range of surgical treatment and the therapeutic effect of radiotherapy. Therefore, chemotherapy plays an important role in the comprehensive treatment of many malignant tumors. At present, there are two problems in the application of chemotherapy drugs commonly used in clinical practice: one is that they have poor recognition of tumor cells and have large toxic and side effects. While killing tumor cells, they will also damage some normal tissue cells; second, they have poor membrane permeability and metabolism in vivo. It is fast, has low bioavailability, and requires multiple injections of large doses to maintain the drug concen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K47/60A61K31/704A61K33/24A61P35/00
CPCA61K9/0092A61K31/704A61K33/24A61K47/02A61K2300/00
Inventor 隋磊杨涛王剑张俊江王娉婷吴珍珍
Owner STOMATOLOGICAL HOSPITAL TIANJIN MEDICAL UNIV
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