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A composite protective agent for superoxide dismutase and its preparation method

A technology of superoxide and protective agent, applied in biochemical equipment and methods, oxidoreductase, drug combination, etc., can solve the problem of not being able to simultaneously improve the activity and stability of superoxide dismutase, and limit the application of superoxide dismutase , the preparation process is not simple enough, etc., to achieve the effect of promoting wound healing, increasing stability, and improving enzyme activity

Active Publication Date: 2020-08-28
FUQING BRANCH OF FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there are the following problems: (1) the use of alcohol-in-oil preparations or solid protective agents cannot simultaneously improve the activity and stability of superoxide dismutase, which greatly limits the application of superoxide dismutase; (2) the preparation process is not simple enough ,higher cost

Method used

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  • A composite protective agent for superoxide dismutase and its preparation method

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Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of superoxide dismutase composite protective agent, is characterized in that, comprises the steps:

[0032] (1) Dissolve positively charged amino acid (arginine) and glucose in ultrapure water at a molar ratio of 1:1 to make a 0.6mol / L solution, place it in a boiling water bath and heat it for 1 hour, after cooling Obtain the stock solution of the reaction product;

[0033] (2) Dissolve the synergist (34.2g of lactose) and 65mL of the stock solution of the reaction product obtained in the above step (1) in ultrapure water, shake well, and set the volume to 1L to obtain the composite protective agent before sterilization;

[0034] (3) The product obtained in the above step (2) was sterilized at 121°C for 20 minutes to obtain sample 1.

Embodiment 2

[0036] A preparation method of superoxide dismutase composite protective agent, is characterized in that, comprises the steps:

[0037] (1) Dissolve positively charged amino acid (arginine) and glucose in ultrapure water at a molar ratio of 1:1 to make a 0.6mol / L solution, place it in a boiling water bath and heat it for 1 hour, after cooling Obtain the stock solution of the reaction product;

[0038] (2) Dissolve the synergist (lactose 34.2g, calcium chloride 11.1g), the stock solution of the reaction product obtained in the above step (1) 65mL in ultrapure water, shake well, and dilute to 1L to obtain the presterilized Composite protective agent;

[0039] (3) The product obtained in the above step (2) was sterilized at 121° C. for 20 minutes to obtain sample 2.

Embodiment 3

[0041] A preparation method of superoxide dismutase composite protective agent, is characterized in that, comprises the steps:

[0042] (1) Dissolve positively charged amino acid (arginine) and glucose in ultrapure water at a molar ratio of 1:1 to make a 0.6mol / L solution, place it in a boiling water bath and heat it for 1 hour, after cooling Obtain the stock solution of the reaction product;

[0043] (2) Dissolve the synergist (lactose 34.2g, aloe polysaccharide 0.2g) and the stock solution of the reaction product obtained in the above step (1) in 65mL in ultrapure water, shake well, and set the volume to 1L to obtain the presterilized Composite protective agent;

[0044] (3) The product obtained in the above step (2) was sterilized at 121°C for 20 minutes to obtain sample 3.

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Abstract

The invention relates to an enzyme composite protective agent and a preparation method thereof. The preparation method comprises the following steps: (1) dissolving positively charged amino acid and glucose in ultra-pure water in the molar ratio of 1: 1, so that a 0.6mol / L solution is prepared; heating the solution in a boiling-water bath for 1h, and conducting cooling so as to obtain a reaction product stock solution; (2) dissolving 34.2-57.5g of synergist and 65mL of the reaction product stock solution prepared by the step (1) in ultra-pure water, uniformly shaking an obtained solution, and setting a constant volume to 1L so as to obtain a composite protective agent before being sterilized; and (3) sterilizing a material obtained in the step (2) at 121 DEG C for 20min, so that the superoxide dismutase composite protective agent is obtained. With the application of the protective agent, enzyme activity is improved by 20%, and moreover, stability is greatly enhanced; and a normal-temperature preservation period is nearly doubled; the protective agent has effects of promoting wound healing and inhibiting bacteria; and such complex and high-energy-consumption steps as freeze-drying and the like are prevented, so that the protective agent is relatively low in cost.

Description

technical field [0001] The invention relates to an enzyme composite protectant and a preparation method thereof, more specifically, the invention relates to a superoxide dismutase composite protector and a preparation method thereof. Background technique [0002] It is known that superoxide dismutase is an antioxidant metalloenzyme existing in organisms, which has the function of catalyzing the dismutation of superoxide anion radicals to generate oxygen and hydrogen peroxide, and plays an important role in the balance of oxidation and antioxidation in the body. [0003] Superoxide dismutase is a pure natural biologically active substance, which has no toxic and side effects on the human body. It belongs to acid protease, and its activity and catalytic efficiency are related to its content and the physical and chemical factors of the environment. The activity of superoxide dismutase has poor stability at room temperature and its biological half-life is short, and it will grad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/44A61K47/26A61K47/18A61K47/02A61K47/36A61K47/10A61K47/12A61P17/02A61P31/04
CPCA61K38/446A61K47/02A61K47/10A61K47/12A61K47/183A61K47/26A61K47/36C12Y115/01001
Inventor 项雷文施源德陈文韬罗冬梅严金红
Owner FUQING BRANCH OF FUJIAN NORMAL UNIV
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