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A fusion lyase for lysing Escherichia coli

A technology of Escherichia coli and colicin, which is applied in the field of fusion lysing enzymes, can solve the problem that Escherichia coli cannot be lysed from the outside

Inactive Publication Date: 2019-07-26
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The present invention discloses a fusion lyase that can lyse Escherichia coli from outside the body. The binding region and recognition region (BR) of Colicin A are fused with the lyase. The fusion protein exhibits a bactericidal effect and can lyse large intestine from outside the bacteria. Bacillus, which solves the problem that current phage lyases cannot lyse E. coli in vitro

Method used

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  • A fusion lyase for lysing Escherichia coli
  • A fusion lyase for lysing Escherichia coli
  • A fusion lyase for lysing Escherichia coli

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Embodiment 1

[0022] 1. Construction and purification of fusion lyase

[0023] The gene of the colicin transit fragment (SEQ NO.1) was cloned by PCR, and the recombinant plasmid pUC-colicin was constructed on the serialized T-vector, and the gene of the phage lyase (SEQ NO.1) was cloned by PCR. The gene of SEQ NO.2), the serial pUC-colicin, and the downstream of the colicin transit fragment were constructed into recombinant plasmid pUC-lysis. The fused colibactin transit fragment and the gene of phage lyase (SEQ NO.3) were excised with endonuclease and recovered. The recovered product was connected to the prokaryotic expression vector pET-28a to construct the recombinant prokaryotic expression vector pET-lysis. Transform pET-lysis into BL21(DE3) condon Plus RIPL. Bacteria were grown to logarithmic growth phase (OD=0.45) at 37°C. After being induced by adding IPTG, the bacterial culture containing the colicin-lysis gene carrier would suddenly become clear at a certain time after adding IPT...

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Abstract

The invention discloses fusion lyase for killing Escherichia coli from outside of bacteria, and also provides a construction method thereof at the same time. Biological technology is used for fusion of a part of a transporting area of Colicin and bacteriophage lysine, that is a combination area of Colicin A, an identification area (BR), and lyase are combined, in order to construct the fusion lyase for directly killing Escherichia coli from outside of bacteria. Fusion protein expresses bactericidal effects, and can carry out lysis of Escherichia coli from outside of bacteria, and the problem that current bacteriophage lyase cannot carry out lysis of Escherichia coli from outside of bacteria is solved.

Description

technical field [0001] The invention discloses a fusion lyase capable of killing Escherichia coli from outside the bacteria, and also provides a construction method thereof, which overcomes the defect that the phage lyase cannot kill Escherichia coli from outside the bacteria. The invention belongs to the field of biopharmaceuticals. Background technique [0002] Escherichia coli is one of the main pathogens that cause digestive tract diseases in humans and animals. Due to the large number and wide non-standard application of antibiotics, a large number of pathogenic Escherichia coli have produced multiple drug resistance. "Super E. coli" has been widespread in pastures and nature. The drug resistance of pathogenic Escherichia coli seriously endangers food safety and public health safety. [0003] Phage and its lytic enzyme preparations are considered to be effective preparations for solving bacterial multidrug resistance. A variety of phage lytic enzyme preparations have...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/70
CPCC07K2319/036C12N9/88C12N15/62C12N15/70
Inventor 雷连成闫广谋王爽吕蒙朱日宁马强顾敬敏孙长江冯新韩文瑜
Owner JILIN UNIV
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