A fusion lyase for lysing Escherichia coli

A technology of Escherichia coli and colicin, which is applied in the field of fusion lysing enzymes, can solve the problem that Escherichia coli cannot be lysed from the outside
CN107033246BInactive Publication Date: 2019-07-26JILIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JILIN UNIV
Publication Date
2019-07-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses fusion lyase for killing Escherichia coli from outside of bacteria, and also provides a construction method thereof at the same time. Biological technology is used for fusion of a part of a transporting area of Colicin and bacteriophage lysine, that is a combination area of Colicin A, an identification area (BR), and lyase are combined, in order to construct the fusion lyase for directly killing Escherichia coli from outside of bacteria. Fusion protein expresses bactericidal effects, and can carry out lysis of Escherichia coli from outside of bacteria, and the problem that current bacteriophage lyase cannot carry out lysis of Escherichia coli from outside of bacteria is solved.
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Description

technical field

[0001] The invention discloses a fusion lyase capable of killing Escherichia coli from outside the bacteria, and also provides a construction method thereof, which overcomes the defect that the phage lyase cannot kill Escherichia coli from outside the bacteria. The invention belongs to the field of biopharmaceuticals. Background technique

[0002] Escherichia coli is one of the main pathogens that cause digestive tract diseases in humans and animals. Due to the large number and wide non-standard application of antibiotics, a large number of pathogenic Escherichia coli have produced multiple drug resistance. "Super E. coli" has been widespread in pastures and nature. The drug resistance of pathogenic Escherichia coli seriously endangers food safety and public health safety.

[0003] Phage and its lytic enzyme preparations are considered to be effective preparations for solving bacterial multidrug resistance. A variety of phage lytic enzyme preparations have...

Claims

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