Malus halliana Koehne flower polysaccharides, extracting method thereof and application of Malus halliana Koehne flower polysaccharides to preparation of coagulation-promoting medicine

A technology of hanging silk crabapple and extraction method, which is applied in the direction of drug combination, blood disease, medical formula, etc.

Active Publication Date: 2017-08-11
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Malus halliana Koehne flower polysaccharides, extracting method thereof and application of Malus halliana Koehne flower polysaccharides to preparation of coagulation-promoting medicine
  • Malus halliana Koehne flower polysaccharides, extracting method thereof and application of Malus halliana Koehne flower polysaccharides to preparation of coagulation-promoting medicine

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Embodiment 1

[0021] Example 1 Extraction of polysaccharides from the flowers of Begonia chinensis

[0022] A method for extracting polysaccharides from the flower of crabapple, which uses the flower of crabapple as a raw material, specifically comprising the following steps:

[0023] 1) Preparation of crude polysaccharides: crush 500 g of Crabapple flowers, and extract them with petroleum ether and 70% ethanol sequentially at room temperature (the amount of petroleum ether and 70% ethanol should be added to immerse the flowers of Crabapple, petroleum ether , 70% ethanol were leached 3 times each, 3 days each time), filtered after the leaching was completed, and the obtained residue was dried. Then hot water extraction was carried out with distilled water at 90°C (the ratio of solid to liquid during extraction was 1 g: 20 g, each extraction was 3 h, and a total of 3 extractions were performed). After the extraction was completed, suction filtration was carried out with a Buchner funnel whi...

Embodiment 2

[0028] Example 2 Anticoagulant test of polysaccharides from Begonia chinensis flower.

[0029] METHODS: Four tests of plasma coagulation in rabbits in vitro.

[0030] Instruments and materials: TGL-16gR high-speed desktop refrigerated centrifuge (Shanghai Anting Scientific Instrument Factory); HF6000-4 semi-automatic coagulation analyzer (Southern Hanfang Medical Instrument Co., Ltd.); LRH-150 biochemical incubator (Shanghai Yiheng Technology Co., Ltd. company); sodium chloride injection; 0.109 mol / L sodium citrate solution, breviscapine for injection (Hunan Hengsheng Pharmaceutical Co., Ltd., 20110202); activated partial thrombin time (APTT) assay kit (1121911), coagulation Enzyme time (PT) assay kit (105295), thrombin time (TT) assay kit (121168), fibrinogen (FIB) content assay kit (132107) were all produced by Shanghai Sun Biotechnology Co., Ltd.

[0031] Experimental animals: male rabbits, weighing 1.8-2.4 kg, purchased from the Henan Provincial Animal Center (Zhengzhou, ...

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Abstract

The invention relates to an extracting method of Malus halliana Koehne flower polysaccharides. The extracting method includes the steps of firstly, crushing Malus halliana Koehne flowers, sequentially using petroleum ether and 70+/-5% ethanol to extract the crushed Malus halliana Koehne flowers under normal temperature, filtering, drying residues, extracting with distilled water, performing vacuum concentration on filtrate, performing alcohol precipitation, drying precipitate, dissolving with distilled water, using an Sevag method to remove protein, performing vacuum concentration on obtained supernate, performing alcohol precipitation again, drying precipitate, dissolving with distilled water, performing dialysis, and performing freeze drying to obtain crude polysaccharides; secondly, dissolving the crude polysaccharides with distilled water, feeding to a DEAE-52 chromatographic column, performing gradient elution with 0.0 mol/L NaCl, 0.1 mol/L NaCl and 0.2 mol/L NaCl, collecting and combining solutions with the same component, performing vacuum concentration, performing dialysis, and performing freeze drying; dissolving with distilled water again, feeding to a Sephadex G-100 chromatographic column, eluting with distilled water, combining solutions with the same component, performing vacuum concentration, and performing freeze drying to obtain the Malus halliana Koehne flower polysaccharides. Coagulation tests show that the Malus halliana Koehne flower polysaccharides have certain in-vitro coagulation-promoting activity and can be used as potential hemostatic medicine.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a polysaccharide from Begonia polysaccharide, an extraction and purification method and its new use in promoting blood coagulation. Background technique [0002] Hanging Begonia ( Malus Halliana Koehne) is a plant of the genus Malus in the Rosaceae family. Hanging silk crabapple flower, slight gas, slightly bitter, astringent taste, calm in nature, enters the liver meridian. Cure mainly blood metrorrhagia, regulate menstruation and blood. Literature research found that: Chuishi Begonia has liver protection, anti-oxidation, and in vitro inhibition α -Glucosidase activity, and there are certain active ingredients in the flowers, leaves and fruits of the crabapple, which have high medicinal value. The research on Begonia chinensis mainly focuses on landscaping, cultivation and breeding, health drinks, biological activity, etc.; the research on its chemical c...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P7/04
CPCA61K31/715C08B37/00C08B37/0003A61K2300/00
Inventor 康文艺余琦辛昕呼谧允王鹏禹
Owner HENAN UNIVERSITY
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