Lactobacillus plantarum and its application

A technology of Lactobacillus plantarum and strains, applied in the direction of Lactobacillus, application, bacteria, etc., can solve the problems of slow growth of lactic acid bacteria and low quality of alfalfa silage, and achieve the effects of accelerating the fermentation process, increasing in vitro digestibility, and improving the quality of silage

Inactive Publication Date: 2017-08-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the problem of low alfalfa silage quality due to slow growth of lactic acid bacteria and low acid production in the silage preparation pro

Method used

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  • Lactobacillus plantarum and its application
  • Lactobacillus plantarum and its application
  • Lactobacillus plantarum and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1: Isolation and strain identification of Lactobacillus plantarum CAU-F1

[0037] 1. Isolation of strains

[0038] Separation of Lactobacillus plantarum by dilution plate method: Weigh 0.5g of feces samples from Holstein middle-period dairy cows, place them in a test tube filled with 4.5mL sterilized physiological saline, shake them well to make them evenly mixed, and the dilution of this test tube is 10 -1 , and then pipette 0.5mL to the next test tube, and dilute the samples to 10 -6 , then draw 0.1mL of the diluted sample and spread it on the MRS medium plate, place it in an anaerobic incubator at 30°C for 48 hours, pick colonies with different shapes and colors on a plate with a suitable colony density, and continue to streak Cultivate until the colony is monotonous, pick a typical colony, and carry out catalase activity determination, Gram staining and microscopic examination. Any strains that are Gram-positive and catalase-negative can be preliminarily i...

Embodiment 2

[0069] Embodiment 2: the preparation of alfalfa silage

[0070] The preparation of alfalfa silage comprises the following steps:

[0071] (1) Dry the alfalfa to a moisture content of about 60%, cut it to 1cm-2cm, and mix well;

[0072] (2) To step (1) alfalfa according to 6log cfu g -1 Lactobacillus plantarum (Lactobacillus plantarum) CAU-F1 was inoculated, and no addition (Control), GFG (commercial bacterial agent), FG1 (commercial bacterial agent) were used as controls, and each treatment was repeated 3 times;

[0073] (3) Put the alfalfa from step (2) into 30cm×20cm polyethylene silage bags, 100g in each bag, pump air with a vacuum sealing machine, seal it, and store it at room temperature for 60 days.

[0074] Nutritional quality, fermentation quality, microbial analysis and in vitro digestion experiments were carried out after alfalfa silage.

[0075] 1. Determination of nutritional quality and fermentation quality of silage:

[0076] After silage, weigh 10g of a repr...

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Abstract

The invention discloses Lactobacillus plantarum and its application, and belongs to the field of silage preparation and processing. The Lactobacillus plantarum CAU-F1, with collection number of CGMCC No. 13610, is used for preparing silage, in particular to lucerne silage. The Lactobacillus plantarum CAU-F1 can rapidly decrease a pH value of silage, accelerate the fermenting process and improve the ensiling rate; besides, the Lactobacillus plantarum can inhibit reproduction of undesirable microorganisms, improve the lucerne ensiling quality, well store the lucerne nutrition, overcome the problem of difficulty in lucerne fermentation, and increase the in-vitro digestibility coefficient of the silage. The ensiling effect of the additive prepared by the Lactobacillus plantarum CAU-F1 is better than that of the marketed lactobacillus additive; moreover, the Lactobacillus plantarum is low in cost, safe and easy to use.

Description

technical field [0001] The invention belongs to the field of silage preparation and processing, in particular to plant lactobacillus and application thereof. Background technique [0002] Silage refers to cutting fresh green fodder into sealed silage facilities (cellars, moats, towers, bags, etc.), and through microbial fermentation, it is made into a juicy feed with a special aroma and rich nutrition. Lactic acid bacteria are the dominant flora of high-quality silage. They reproduce in large numbers under anaerobic conditions, produce a large amount of lactic acid, and inhibit the growth of harmful bacteria, thereby making excellent silage. In the early stage of silage fermentation, Leuconostoc and Enterococcus multiplied rapidly, producing a certain amount of lactic acid, and the pH dropped, creating conditions for other lactic acid bacteria and Peptococcus; in the middle and late stages of fermentation, Lactobacillus plantarum dominated, and some other lactic acid bacteri...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/12A23K30/18C12R1/25
CPCC12R2001/25C12N1/205A23V2400/169
Inventor 杨富裕姚丹丹李东霞张颖超玉柱
Owner CHINA AGRI UNIV
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