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Bacteriocin-producing lactobacillus plantarum and compound application of lactobacillus plantarum in silage

A technology of Lactobacillus plantarum and silage, which is applied in the field of bacteriocin-producing Lactobacillus plantarum and its compound application in silage, and can solve problems such as inapplicability

Active Publication Date: 2022-02-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, previous studies mostly focused on the inhibition of Gram-positive bacteria in silage, and were not applicable to the inhibition of Gram-negative bacteria such as Escherichia coli and other harmful bacteria in the silage process.

Method used

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  • Bacteriocin-producing lactobacillus plantarum and compound application of lactobacillus plantarum in silage
  • Bacteriocin-producing lactobacillus plantarum and compound application of lactobacillus plantarum in silage
  • Bacteriocin-producing lactobacillus plantarum and compound application of lactobacillus plantarum in silage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1. Isolation and identification of Lactobacillus plantarum CAU-PL28 and CAU-S7

[0075] 1. Isolation of Lactobacillus plantarum

[0076] Separation of Lactobacillus plantarum by dilution plate method: Weigh 20 g of mulberry silage under aseptic conditions, place it in a conical flask filled with 180 mL of sterile distilled water, shake well, and obtain a 10-fold diluted sample suspension, then press The same 10-fold dilution method was directly serially diluted to 10 -6 . Select an appropriate dilution, take 0.1 mL and evenly spread it on the MRS solid medium plate, incubate in a 30°C incubator for 48 hours, and count the plates. According to the characteristics of colony size, shape and color, typical colonies were picked for Gram staining microscopy and catalase test. All strains that are Gram-positive and catalase-negative can be initially identified as lactic acid bacteria. They are streaked and purified twice on MRS medium, inoculated into MRS solid slan...

Embodiment 2

[0126] Example 2, the application effect of Lactobacillus plantarum CAU-PL28 and Lactobacillus plantarum CAU-S7 added to silage alone or in combination

[0127] 1. Preparation of silage (taking woody feed tree as an example)

[0128] After the first crop of paper mulberry is cut, the undried whole mulberry tree is shortened to about 2 cm, and mixed evenly to obtain silage raw materials.

[0129] 2. Preparation of Lactobacillus plantarum CAU-PL28 and Lactobacillus plantarum CAU-S7 bacterial agents

[0130] Lactobacillus plantarum CAU-PL28 and Lactobacillus plantarum CAU-S7 were respectively inoculated (inoculum number of live bacteria 1:1) in liquid MRS medium (pH value 6.5), 30°C, 150rpm shaking culture for 24h, respectively to obtain plants The bacterial liquid of Lactobacillus CAU-PL28 and the bacterial liquid of Lactobacillus plantarum CAU-S7. The cells of the two bacterial solutions were collected and freeze-dried respectively to prepare two bacterial powders. Two bacte...

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Abstract

The invention discloses a bacteriocin-producing lactobacillus plantarum and a compound application of the bacteriocin-producing lactobacillus plantarum in silage. The active ingredients of the composite microbial agent provided by the invention consist of: lactobacillus plantarum (Lactobacillus plantarum) CAU-PL28 and lactobacillus plantarum (Lactobacillus plantarum) CAU-S7. The registration number of the CAU-PL28 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.20503. The registration number of the CAU-S7 in the China General Microbiological Culture Collection Center is CGMCC No.20502. Experiments prove that the complex microbial inoculant has a good effect of fermenting broussonetia papyrifera silage, can rapidly inhibit escherichia coli proliferation, reduce the pH value of silage, accelerate the fermentation process, significantly reduce protein degradation and preserve broussonetia papyrifera nutrition, and can be widely used.

Description

technical field [0001] The invention belongs to the technical field of silage processing, and in particular relates to a bacteriocin-producing lactobacillus plantarum and its compound application in silage. Background technique [0002] With the increase of population and continuous improvement of living standards, people's demand for meat, eggs and milk has increased significantly. The rapid development of animal husbandry has increased the demand for high-quality forage grass year by year. Silage is a common method of forage preservation. However, raw materials such as legumes or woody feed have high moisture and protein content, and harmful bacteria are prone to multiply in large numbers during the silage process. Traditionally, it is believed that as long as lactic acid bacteria ferment to produce organic acids such as lactic acid during the silage process, so as to maintain a low pH value and anaerobic conditions, the silage can be guaranteed to be free of pathogenic ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K30/18C12R1/25
CPCC12N1/20A23K30/18A23V2400/169Y02P60/87
Inventor 杨富裕郭琳娜倪奎奎李小梅陈菲
Owner CHINA AGRI UNIV
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