DNA methylation index for early diagnosis and risk assessment of cancer and its application

A methylation and application technology, applied to DNA methylation indicators for early diagnosis and risk assessment of cancer and their application fields, can solve problems such as inability to detect

Active Publication Date: 2021-04-06
TIANJIN MEDICAL UNIV CANCER INST & HOSPITAL
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Tumors at the primary site release limited cfDNA into the blood, so mutation detection to detect early-stage cancer is challenging
Additionally, driver events in early cancers may be epigenetic in nature and may not be detectable based on genetic mutations

Method used

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  • DNA methylation index for early diagnosis and risk assessment of cancer and its application
  • DNA methylation index for early diagnosis and risk assessment of cancer and its application
  • DNA methylation index for early diagnosis and risk assessment of cancer and its application

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Embodiment

[0068] Hereinafter, the present invention will be described in detail with reference to Examples, which are only intended to illustrate the present invention and are not intended to limit the scope of the present invention. The scope of the invention is specifically defined by the appended claims.

[0069] Materials and methods

[0070] research population

[0071] Ovarian cancer cases were women with primary epithelial ovarian cancer aged 25-85 years old who were diagnosed by histological diagnosis in Tianjin Medical University Cancer Hospital from 2007 to 2013 (all of them were Han Chinese). Cases with a history of cancer treatment or blood transfusion 6 months before diagnosis were excluded. The blood samples of the case group were collected in the operating room, and blood counts and coagulation factor values ​​were obtained by blood routine and coagulation tests, and these were all performed within one week before the operation. The control group consisted of tumor-fre...

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Abstract

The present invention relates to a method for cancer diagnosis and / or risk assessment of cancer in a subject, said method comprising the following steps: a) determining the The DNA methylation status at at least one DNA methylation site in; b) and compare the DNA methylation status at the DNA methylation site in the sample from the subject with that from the healthy control group The DNA methylation status at the corresponding DNA methylation site in the sample is compared, if there is a significant difference in the methylation status between the above two, it indicates that the subject has cancer or is suffering from at risk of cancer. The present invention also provides kits for the above methods.

Description

technical field [0001] The present invention relates to a method for cancer diagnosis and / or cancer risk assessment in a subject and a kit thereof. Background technique [0002] Ovarian cancer is the deadliest gynecological tumor worldwide (1) ((1) is the reference number, the same below). There are two main reasons for the high fatality rate: lack of early detection means and chemotherapy resistance (2). Although initial chemotherapy is effective in ovarian cancer patients, approximately 25% of patients develop chemotherapy resistance within six months (3). In-depth studies of tumor tissues have revealed diverse genetic and epigenetic changes in the development and progression of ovarian cancer. Discovering effective markers for early detection, especially in blood, is urgently needed to improve the prognosis of ovarian cancer. [0003] Lysis of tumor cells is known to release DNA fragments (cfDNA, cell-free DNA) and other molecules such as miRNAs and cytokines into the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/154
Inventor 陈可欣李莲郑红黄育北
Owner TIANJIN MEDICAL UNIV CANCER INST & HOSPITAL
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