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Application of lysosome positioning fluorescence probe in near-infrared ratio detection of hydrazine

A technology of lysosome positioning and fluorescent probes, which is applied in the field of fluorescent sensing in analytical chemistry, can solve the problem of fewer fluorescent probes for cell positioning, and achieve good detection results, obvious changes in fluorescence, and mild synthesis conditions

Inactive Publication Date: 2017-08-11
ZHENGZHOU UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few fluorescent probes for detecting hydrazine that can achieve near-infrared ratiometric fluorescence detection, especially fewer fluorescent probes that can achieve cell localization

Method used

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  • Application of lysosome positioning fluorescence probe in near-infrared ratio detection of hydrazine
  • Application of lysosome positioning fluorescence probe in near-infrared ratio detection of hydrazine
  • Application of lysosome positioning fluorescence probe in near-infrared ratio detection of hydrazine

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Experimental program
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Embodiment 1

[0036] The fluorescent probe in this example adopts the following synthetic route:

[0037]

[0038] 1) Synthesis of 3-pyrrolidinyl-1-phenol:

[0039] 3-Aminophenol (1.0913 g, 10.0 mmol), potassium carbonate (1.5203 ​​g, 11.0 mmol) and 1,4-dibromobutane (1340 μL, 11.0 mmol) were dissolved in 10 mL of DMF. Heated to 80°C, reacted for 2 hours, and cooled to room temperature. Purified by column (developing solvent: ethyl acetate:petroleum ether=1:10) to obtain 3-pyrrolidinylphenol, yield: 58.8%.

[0040] Characterized as follows: 1 H NMR (400MHz, DMSO-d 6 , TMS): δ H 7.10(t, 1H), 6.19(t, 2H), 6.09(s, 1H), 4.86(s, 1H), 3.28(t, 4H), 2.02(t, 4H). 13 C NMR (100MHz, DMSO-d 6 ):δ C 156.55, 149.47, 130.06, 104.81, 102.57, 98.69, 47.71, and 25.44.

[0041] 2) Synthesis of 2-nitroso-5-pyrrolidine-1-phenol

[0042] 3-Pyrrolidinylphenol (324.6 mg, 2 mmol) was dissolved in 12 ml of concentrated hydrochloric acid (37 wt%) and 4 mL of water mixed solvent, and 4 ml of aqueous soluti...

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Abstract

The invention belongs to an application of a lysosome positioning fluorescence probe in near-infrared ratio detection of hydrazine. A chemical structural formula of the probe is shown in the specification. The synthesis condition of the fluorescence probe is mild, the cost is lower, and the fluorescence probe has good selectivity and anti-interference performance for hydrazine, is good in time and temperature stability and has actual application value in the field of biological chemistry.

Description

technical field [0001] The invention belongs to the technical field of fluorescence sensing in analytical chemistry, and specifically relates to a lysosome-localized fluorescent probe, a preparation method and an application in near-infrared ratiometric detection of hydrazine. Background technique [0002] Hydrazine hydrate can be used as a high-energy propellant for rocket propulsion systems. At the same time, hydrazine hydrate has strong reducing and basic properties, which makes hydrazine hydrate also have good applications in industrial fields, such as in metal anticorrosion and in the synthesis of different types of polymers. As its common precursor, as an intermediate for textile dyes and pharmaceutical preparations, etc. At the same time, hydrazine hydrate is highly toxic, and will cause great harm to the human body when the human body inhales or touches hydrazine hydrate through the skin. Hydrazine hydrate is a neurotoxin and has a strong mutagenic effect, causing s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6417
Inventor 李占先班亚楠于明明杜玮玮李海霞魏柳荷
Owner ZHENGZHOU UNIV
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