Tobacco variety drought tolerance rapid identification method

Abstract

The invention relates to a tobacco variety drought tolerance rapid identification method. The tobacco variety drought tolerance rapid identification method includes: 1) adding sterilized river sand in a culture dish, wherein the height of the river sand is not greater than 1/3 of the height of the culture dish; and adding 15% PEG solution, and using distilled water as a contrast; 2) laying a layer of filter paper on a surface of the river sand, putting sterilized tobacco seeds on the filter paper, covering the culture dish with a culture dish cover, performing sealing, and putting the culture dish in a culture box to perform routine culture; 3) surveying the germination rate every day, calculating a germination index after surveying is finished in the 14-th day, measuring the lengths of roots, the heights of stems, and the fresh weight, calculating a relative germination index A, a relative root length B, a relative stem height C and a relative fresh weight D, using an average value of the A, B, C, and D as a drought stress index of the tobacco variety, wherein the larger the drought stress index, the stronger the drought tolerance of the tobacco variety. The method is convenient and fast in test, is not limited by testers and places, and is suitable for drought tolerance identification of tobacco germplasm resources.

Description

technical field [0001] The invention belongs to the technical field of tobacco identification and processing, and in particular relates to a rapid identification method for drought tolerance of tobacco varieties. Background technique [0002] Tobacco is one of the important economic crops, especially flue-cured tobacco. The area and output of flue-cured tobacco in my country rank first in the world. At present, my country's tobacco production areas are mainly concentrated in hilly areas with poor irrigation conditions. The shortage and imbalance of water resources is an important factor restricting the development of modern tobacco agriculture. Different flue-cured tobacco varieties have different drought tolerance and are affected by drought in different degrees. Therefore, it is particularly important to select drought-tolerant varieties. [0003] Drought tolerance identification technology has been studied more in other crops such as wheat and corn. Due to different cro...

AI Technical Summary

Problems solved by technology

The main performances are as follows: 1) Potted water control tests are mostly used for the identification of drought tolerance of existing tobacco varieties. Although the results are reliable, the cycle is long, time-consuming and laborious, and it is only suitable for the identification of drought tolerance of individual varieties, not suitable for large-scale tests

2) Wang Rengang et al. disclosed a "method for identifying drought resistance of tobacco at the seedling stage (CN201110106454.3)". The identification of large fields is relatively fast and labor-saving, but it needs to be carried out in a greenhouse and has certain limitations: ① Special facilities are required ② It is necessary to observe the drought situation of tobacco seedlings manually, and each person has a different judgment on the wilting situation. Therefore, it is difficult to grasp the rehydration time; ③ It needs repeated water control and rehydration, which requires a lot of manpower and material resources, and is not suitable for large-scale identification of tobacco. Variety Drought Tolerance

3 There are many drought tolerance identification methods and indicators, and there is no unified identification standard

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