Analysis method of diversity and difference of intestinal and oral flora

Abstract

The invention provides a method for analyzing the diversity and the differences of intestinal and oral florae based on a new generation of high-throughput sequencing technology. The method comprises the following steps of (1) collecting a to-be-detected sample and carrying out microbial genome DNA extraction to obtain a genome DNA extracting solution; (2) detecting the genome DNA extracting solution to determine the concentration and the purity of a microbial genome DNA in the sample; (3) carrying out PCR amplification on the genome DNA extracting solution by using 16SrDNA-specific V3-V4 region primers and carrying out library construction; (4) sequencing by using an IlluminaMiSeq sequencing system; and (5) carrying out bioinformatics analysis by using FLASH, QIIME and usearch61. Compared with a traditional flora detection method, by using the new generation of high-throughput technology, compositions and functions of the florae can be analyzed more deeply in more detail.

Description

technical field [0001] The invention relates to a technology and method for detecting the diversity and difference of intestinal and oral microorganisms of disease patients based on a new generation of high-throughput sequencing technology. Background technique [0002] Most of the physiological functions of the human body are the result of the symbiotic life formed by the co-evolution of the human body and the microbiome. There are a variety of normal microbial communities in a healthy human body. They have a symbiotic relationship with the host and jointly maintain the physiological balance of the host. . Once the homeostasis of symbiosis between the host and microorganisms is broken, a variety of human diseases will be induced. It has been recognized that the gut and oral microbes are closely related to human health and diseases. Therefore, it is not enough to study human diseases only from the perspective of human beings. The role of gut microbes that live in symbiosis ...

AI Technical Summary

Problems solved by technology

[0003] The traditional method of studying microorganisms mainly relies on the isolation and culture technology, but the bacteria that can be cultivated in the human body only account for a small part of the total number of bacteria, and most of the microorganisms are difficult to culture, so it is difficult for us to use the current isolation and culture technology to gain a deep understanding of the human body. The overall structure of the flora and the interrelationships between their hosts

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