44 results about "High throughput technology" patented technology

The invention relates to a method for screening drug target genes based on a CRISPR/Cas9 high-throughput technology. The method comprises the steps of firstly establishing a sgRNA library; secondly packaging the sgRNA library by using slow viruses, and collecting the viruses; thirdly screening the sgRNA library in a cancer cell line; fourthly extracting cells obtained by screening and genome DNA of the cells before screening; and finally enriching sgRNA in genome DNA. Compared with the prior art, the method has the advantages that a CRISPR/Cas cell screening process is improved, the virus infection efficiency is determined with a simple and convenient method by utilizing puromycin resistance of infected cells, and MOI values of the viruses are determined; more importantly, a virus packaging method is greatly optimized, so that the virus packaging efficiency is improved to be more than 5 times that of a conventional method, and large-scale drug target screening cost can be greatly reduced; and the method is used for promoting industrialization of cancer drug target screening.

The invention provides a method for analyzing the diversity and the differences of intestinal and oral florae based on a new generation of high-throughput sequencing technology. The method comprises the following steps of (1) collecting a to-be-detected sample and carrying out microbial genome DNA extraction to obtain a genome DNA extracting solution; (2) detecting the genome DNA extracting solution to determine the concentration and the purity of a microbial genome DNA in the sample; (3) carrying out PCR amplification on the genome DNA extracting solution by using 16SrDNA-specific V3-V4 region primers and carrying out library construction; (4) sequencing by using an IlluminaMiSeq sequencing system; and (5) carrying out bioinformatics analysis by using FLASH, QIIME and usearch61. Compared with a traditional flora detection method, by using the new generation of high-throughput technology, compositions and functions of the florae can be analyzed more deeply in more detail.

The present invention provides a method and a system for processing a plurality of ILs, wherein the processing comprises one or more selected from the group of synthesis, separation, detection, purification, determination and so on, and the methods and systems for processing applies high throughput technique. By using the high throughput technique, the present invention has advantages like being capable of processing a plurality of ILs to attain large amount of experimental data in a short time, which provides great help for IL in large scale industrial and other aspects applications.

The invention discloses a gradient Nb-Si based alloy film preparation method adopting a high flux technology. The preparation method comprises following steps: (1) preparing a Nb-16Si-6Cr-24Ti-6Al matrix; (2) preparing a Cr target material; (3) preparing a xNb-ySi-zTi-kAl target material, x=55-65, y=14-19, z=18-25, and k=1-4, and the numbers are all atomic percentages; (4) installing the Nb-16Si-6Cr-24Ti-6Al matrix prepared in the step (1) on the clamp of a magnetron sputtering facility, placing the Cr target material prepared in the step (2) on a common DC target, putting the xNb-ySi-zTi-kAltarget material prepared in the step (3) on a radio frequency target, and preparing a gradient Nb-Si based alloy film by a magnetron sputtering technology. The method can prepare a great number of Nb-Si based alloys with different ratios, the alloys can be applied to performance tests so as to pick out an optimal ratio, and the work efficiency is improved.

The invention belongs to the field of molecular biology, and particularly discloses a method for detecting a CTC (circulating tumor cell) surface marker molecule GPC3 (glypican-3). The method comprises the following steps: performing erythrocyte splitting on blood, spreading and enriching all of left karyotes (mainly comprising CTCs and lymphocytes) on a nano-substrate for fixation by virtue of a nanotechnology, marking all the cells by virtue of a nucleus fluorescent dye DAPI, performing positive selection by virtue of a tumor immune fluorescent marker, i.e. a cytokeratin antibody anti-CK, and since a CTC surface has a specific expression of the GPC3, incubating all the cells by virtue of a GPC3 primary antibody, performing incubation by virtue of a secondary antibody marked with an FITC fluorescence group, and finally performing scanning by virtue of a high-throughput technology. Compared with the prior art, the detection method has the advantages of convenience, rapidness, economy, high sensitivity and specificity and capability of overcoming the defects of large sample amount requirement and incomplete reaction of GPC3 detection in the prior art.

The invention discloses a high-throughput method based on DNA (desoxyribonucleic acid) and RNA (ribonucleic acid) gene mutation detection. The method comprises the steps that (1) desoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are extracted from a biological sample collected from a subject through utilizing a reagent, and a DNA library is constructed based on desoxyribonucleic acid; ribonucleic acid is reversely transcribed into double-stranded cDNA firstly, and then is constructed into an RNA library; (2) a first probe set is mixed with the DNA library, and meanwhile, a second probe set is mixed with the RNA library; (3) a complex of probes and nucleic acid is separated and obtained from mixtures obtained in step (2) by utilizing markers in the first probe set and second probe set; (4) sequencing is performed on target nucleic acid in the complex by utilizing a high-throughput technique. The method is based on NGS methodology and optimizes the NGS methodology, so that the specificity and sensitivity of detection are further improved. In addition, a DNA detection result can also be verified by detecting RNA.

The invention relates to a molecular structure of a directed evolution soluble pyrroloquiniline quinine-dependent glucose dehydrogenase (s-GDH) and a method for screening the thermostable mutant by adopting the high-throughput technology. The method is characterized by comprising the following steps: a high-capacity mutation library is constructed through an error-prone polymerase chain reaction (PCR) of the s-GDH, the filter paper soaked with a colored substrate is used to perform in-situ screening on a colony plate, and the colony direct conversion method is combined to obtain the mutant ofthe s-GDH, of which thermostability is increased. Compared with the wild-type s-GDH, the thermostability of the obtained s-GDH mutant is obviously increased. The obtained soluble pyrroloquiniline quinine-dependent glucose dehydrogenase with high thermostability lays the foundation for the further expansion of the application range of glucose dehydrogenase.

The invention provides an analysis method for the diversity of oral cavity flora based on a second-generation high throughput sequencing technology and a disease-associated flora marker. The method comprises the following steps of (1) extracting a DNA of a to-be-detected saliva sample; (2) preparing a PCR reaction system, and carrying out PCR amplified reaction of V1-V2 regions of bacteria 16S rDNA, wherein the PCR reaction system comprises a PCR amplification template, a universal primer pair and the like; (3) carrying out detection of a target segment of a PCR product and purification of the PCR product; (4) carrying out second-generation high throughput sequencing on the purified PCR product and detecting the species of microorganisms in the saliva sample; (5) sequencing by using an Ion Torrent PGM sequencing system; and (6) carrying out bioinformatics analysis by using a FLASH, QIIME, usearch61. The diversity and the difference of the oral cavity flora of a patient are detected through the second-generation high throughput sequencing technology, so that the analysis method has the advantages that the composition and the functions of the flora can be more deeply analyzed in more detail by using the new-generation high throughput technology in comparison with a traditional flora detection method.

The invention discloses a calculation method for ideal strength of a two-dimensional material under an electrochemical polarization condition, and belongs to the technical field of two-dimensional materials. By analyzing a rotation axis of the two-dimensional material, the calculation method rotates a system along the rotation axis, a structure after rotating each angle is stored, the structure isstretched uniaxially and biaxially respectively, and ideal strength under each angle is calculated. An ideal strength database of two-dimensional materials under the electrochemical polarization condition is established by high-flux calculation, and the two-dimensional material with the highest ideal strength under the electrochemical polarization condition is selected. The method comprises constructing a two-dimensional material system under the electrochemical polarization condition, realizes calculation of the ideal strength in any direction, and calculates the ideal strength of the two-dimensional material under the electrochemical polarization condition by using a high-flux technology. The method can more precisely screen the two-dimensional material with excellent mechanical properties, and provides favorable assistance for design of new materials.

The invention discloses an electrochromic device for screening solid inorganic electrochromic materials at high throughput and a manufacturing method of the electrochromic device. The problem that optimal electrochromic materials cannot be accurately screened out is solved. The electrochromic device comprises a substrate, a lower transparent conducting layer and multiple electrochromic device units on the lower transparent conducting layer. The units are divided into at least two groups, and each unit is provided with a cathode electrochromic layer, a solid electrolyte layer, an anode electrochromic layer and an upper transparent conducting layer from inside to outside. A thickness gradually-changing layer is formed on at least one functional layer of the cathode electrochromic layers, the solid electrolyte layers and the anode electrochromic layers. When the functional layers are made of one material, the thicknesses of the same functional layers in the units in different groups are different, and the thickness gradually-changing layers are formed. When the functional layers are made of two or more materials, the thickness ratios of the same functional layers in the units in different groups are different, and composite thickness gradually-changing layers are formed. The electrochromic device is used for the high throughput technology and can accurately screen out the optimal electrochromic materials.

The present invention relates to biomarkers useful for detection of types, grades and stages of human breast cancer. The present invention particularly relates to the development of these identified biomarkers as a miRNA chip for the early and accurate diagnosis of human breast cancer. This patent application highlights the novelty in the utility of these miRNAs, that they could be used as a diagnostic kit (miRNA chip) for early and accurate detection of breast cancer grades, stages and subtypes. Few to hundreds of samples can be checked within a span of 2 to 3 hrs and hence this becomes an easy, fast, robust and high throughput technology for screening program for early detection of breast cancer.

The invention provides a method for analyzing children autism intestinal flora diversity and a specific primer pair. The method comprises the following steps: 1) extracting DNA of a to-be-detected stool sample; 2) configuring a PCR reaction system and performing PCR amplified reaction in bacteria 16S rDNA V1-V2 areas, wherein the PCR reaction system comprises a PCR amplification template, a universal primer pair and the like; 3) detecting a target fragment of a PCR product and purifying the PCR product; 4) performing second-generation high-throughput sequencing on the purified PCR product and detecting the types of microorganisms in the stool sample; 5) sequencing by utilizing an I11umina MiSeq sequencing system; 6) performing bio-information analysis by utilizing FLASH, QIIME and usearch61. The method provided by the invention has the benefits that through a second-generation high-throughput sequencing technology, the intestinal flora diversity of a diseased patient and the difference are detected; compared with a traditional flora detection method, the compositions and the functions of floras can be more detailedly and deeply analyzed by applying the new-generation high-throughput technology.

The invention relates to the field of medicines and particularly discloses a screening method of main-effect medicine components in a traditional Chinese medicine compound. The screening method includes the following steps: (1) grouping experimental animals and delivering medicines to the experimental animals; (2) finding a target point gene; (3) calculating influence value; (4) calculating the ratio value of medicine effects and determining a main effect component; and (5) determining the main-effect medicine components in the traditional Chinese medicine compound according to the ratio value of the medicine effect of single medicine. The method not only is closely combined with a modern high-throughput technology being mature and reliable, but also conforms to the theories in traditional Chinese medicine and modern medicine and pharmacology, so that researching on the effective substances in the traditional Chinese medicine compound is more scientific and modern, thereby providing an important basis for further researching a combined medicine.

The invention relates to the technical field of gene engineering, in particular to an SSR (simple sequence repeat) molecular marker of akebia trifoliate as well as an application and a preparation method of the SSR molecular marker. Primers of the SSR molecular marker comprise one or more pairs of primers shown in sequences SEQ ID NO:1-98. The developed transcriptome SSR molecular marker of the akebia trifoliate can be used for analyzing genetic diversity of akebia trifoliate germplasm resources and lays a good theoretical foundation for molecular biology and genetic research of the akebia trifoliate. The SSR marker developed with a transcriptome high-throughput technology is high in speed, low in cost and high in efficiency.

The invention discloses a micro-fluidic chip system for improving a radiation flux of a single ion beam. The micro-fluidic chip system comprises a culture medium storage tank and a micro channel, wherein the micro channel only allows a single cell to pass through in a queue manner, a real-time detection cell electrode is respectively installed at two sides of the micro channel, a radiation point is arranged in front of the real-time detection cell electrodes, and the thickness of the radiation point is less than or equal to 5 micrometers; the real-time detection cell electrode pair generate a differential signal, the micro-fluidic impedance variation in the channel is detected by virtue of the signal processing technology so as to rapidly identify whether the cell passes through or not, a cell identification signal accesses a beam control system of a single ion beam device, so that when the cell passes through the radiation point, the beam control system can control ions to accurately and timely radiate the cell. The micro-fluidics are used for the single ion beam radiation device for the first time, the radiation flux is improved by several tens of times, the requirement on the large dosage of cells for the biology experiment can be met, a novel high-flux technical platform is provided for the radiation biology, and the commercial application prospect is vast.

The invention discloses a high-throughput device for detecting peri-implantitis flora. The device comprises a high-throughput box, a sliding door and a controller, wherein the sliding door and the controller are placed on the left side and the right side of the high-throughput box respectively, the sliding door is connected with the high-throughput box through sliding rails in a sliding mode, thecontroller is detachably connected with the high-throughput box through bolts, the high-throughput box is internally provided with a workbench, a thermometer and a hygrometer, the workbench is horizontally placed on the bottom of the high-throughput box, a photometer, a centrifugal machine and a DNA extraction device are arranged on the upper part of the workbench, the photometer, the centrifugalmachine and the DNA extraction device are horizontally placed on the surface of the workbench, and the workbench is internally provided with a liquid treatment device and a sterile storage chamber. According to the high-throughput device for detecting the peri-implantitis flora, by adopting a biochip high-throughput technology, change of protein and genes of body fluid of saliva or gingival crevicular fluid or the like of a tooth implantation patient can be detected, and the susceptibility of the patient to peri-implantitis is identified.

The present invention provides a method of determining bacterial metastructure by integrating multiple genome-scale information yielded by high-throughput technologies. The metastructure constructs a universal metabolic engineering platform enabling a rational design of bacterial strains through optimization of gene and protein expression.

The invention provides a primer group and kit for detecting drug resistance genes of streptococcus suis based on multiple PCR-DHPLC and an application of the primer group and the kit, and belongs to the technical field of veterinary clinical detection. The primer group for detecting the drug resistance gene of streptococcus suis based on multiple PCR-DHPLC comprises a primer pair for amplifying anermA gene, an ermB gene, a mefA gene and an msrD gene. The primer group provided by the invention has relatively strong specificity and can be used for PCR amplification and DHPLC analysis. The application of the primer group or the kit in streptococcus suis drug resistance gene detection aims at detection of four pairs of drug resistance genes of streptococcus suis macrolide-resistant drugs, candetect four target genes at the same time, meets the requirements of a rapid high-throughput technology, and is good in detection specificity, high in sensitivity and high in sensitivity. The kit canbe used for clinically detecting drug-resistant genes of four macrolide-resistant drugs, namely ermA, ermB, mefA and msrD, of streptococcus suis.

The present invention provides methods and systems for analyzing mammalian transcriptomes, particularly, for low abundant transcripts, and with the use of high throughput technologies. Heptamer primers and sequence tags generated by the iterative randomized algorithm, as well as the sequencing-library generation system for amplifying and synthesis-based sequencing low abundant transcripts using the heptamer primers are also provided. The present invention further provides the use of the invention system and method for identifying key embryological lineage specific transcripts that anticipate differentiation of specific cell types.

The invention belongs to the technical field of biology, and particularly relates to an antibody combination established aiming at a tumor marker and an ELISA method thereof. Compared with an antibody, an antibody analogue has the advantages of having good solubleness, good tissue penetration, good heat stability, good enzyme stability, low production cost and the like. Therefore, the invention discloses the antibody combination established aiming at the tumor marker, wherein the combination comprises stable and specific a pairing antibody analogue, and is expected to replace conventional antibody and be applied in a high-throughout technology (antibody chip) in screening antibody analogue libraries of various kinds to conduct development of antibody analogue bonded reagents.

The invention discloses a clamper and relates to the field of the high throughput technology. The clamper solves the technical problem that the existing clamper cannot integrate a charging/discharging system and a clamping system. The clamper comprises an upper base which is provided with a first through hole, a lower base which is provided with a second through hole, a clamping jaw which is arranged on the lower surface of the lower base, a driving shaft which is connected to the clamping jaw and is used for driving the clamping jaw to clamp a work piece, and a burdening assembly which is used for burdening of the work piece through the first and second through holes. The charging/discharging system and the clamping system are integrated so that a running and positioning device of the experimental system is avoided, an ineffective running process of the experimental device is avoided and experimental effects are improved.