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Method for identifying Chinese medicinal based on cytology dynamic information integration

A technology of dynamic information and cytology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of authentic medicinal materials, good prescriptions of traditional Chinese medicine, credit crisis of Chinese medicinal materials market, etc.

Inactive Publication Date: 2008-05-21
柯传奎
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Authentic medicinal materials are unscrupulous, even on the verge of extinction, and the imagination of good Chinese medicine prescriptions but poor curative effects often occurs, leading to a credit crisis in the domestic and foreign Chinese medicinal materials markets.
The problem is that there is no practical and effective method to identify authentic medicinal materials

Method used

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  • Method for identifying Chinese medicinal based on cytology dynamic information integration
  • Method for identifying Chinese medicinal based on cytology dynamic information integration
  • Method for identifying Chinese medicinal based on cytology dynamic information integration

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0101] Example 1: Cell dynamic information of authentic medicinal material (Cordyceps sinensis) captured from different cell lines

[0102] 1. Take a 96-well detection plate, add 50ul A549, RBL, COS7, COS1, 3T3, C2C12, HELA, HepG2, MCF7, MCF7 / Adr cell line culture medium to each well, measure the baseline, insert A549, RBL, COS7, COS1, 3T3, C2C12, HELA, HepG2, MCF7, MCF7 / Adr cells, so that the cell concentration per well is 1000-10000 / 100ul, placed on the test bench and put into the carbon dioxide incubator to start detection. After the cells grow for 18-24 hours, the cells are placed in the serum-free medium for 2 hours, and then the samples to be tested are added.

[0103] 2. Extraction of Chinese herbal medicines

[0104] Take the Cordyceps sinensis medicinal material, dry it, powder it, and weigh 0.09g. Add 1mL of pure water, soak for 30 minutes, sonicate for 10 minutes, heat in boiling water for 20 minutes, centrifuge at 3500 rpm for 15 minutes, and take the supernatant...

example 2 9

[0107] Examples 2-9: The authentic medicinal materials "Zhe Bawei" (Hangbaiju, Zhe Fritillaria, Wenyujin, Yuanhu, Hangbaizhu, Scrophulariaceae, Hangbai Shao, Zhejiang Ophiopogon japonicus) captured from A549 cell lines are the same as the corresponding Cell dynamic information of Chinese herbal medicines with different origins

[0108] 1. Take a 96-well assay plate, add 50ul of A549 cell line culture medium to each well, test the baseline, insert A549 cells, make the cell concentration of each well 1000-10000 / 100ul, put it on the test bench and put it into the carbon dioxide incubator to start detection. After the cells grow for 18-24 hours, the cells are placed in the serum-free medium for 2 hours and then the samples to be tested are added.

[0109] 2. Extraction of Chinese herbal medicines

[0110] Take Chinese medicinal materials (chrysanthemum, fritillaria, turmeric, Yuanhu, Atractylodes macrocephala, Scrophulariae, Radix Paeoniae Alba, Radix Ophiopogon japonicus) from ...

example 10

[0113] Example 10: 41 categories of 54 kinds of Chinese herbal medicines captured from A549 cell line, including the cell dynamic information of Chinese herbal medicines with the same name and different origins

[0114] 1. Take a 96-well assay plate, add 50ul of A549 cell line culture medium to each well, test the baseline, insert A549 cells, make the cell concentration of each well 1000-10000 / 100ul, put it on the test bench and put it into the carbon dioxide incubator to start detection. After the cells grow for 18-24 hours, the cells are placed in the serum-free medium for 2 hours and then the samples to be tested are added.

[0115] 2. Extraction of Chinese herbal medicines

[0116] Take 54 kinds of Chinese medicinal materials (Ephedra, Yu Baizhi, Guan Dali, Anemarrhena, Chuanhuanglian, Honeysuckle, Banlangen, Artemisia annua, Rhubarb, Hemp Seed, Duhuo, Gentiana officinalis, Wujiapi, Patchouli, Atractylodes, Poria, Carnation Qianzi, capillary, aconite, clove, hawthorn, be...

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Abstract

A high flux technical method for selecting model in a fast minimum way in vitro which is based on the modern molecular biology and cytology; the dynamic information of various cytology is captured by detectors which are sold on market from one or a plurality of tissue and organ cells which are separated from human body, animals, plants or microbes and the information is converted into bar code expression consisting of mathematic matrix or number or letter through the analysis method of the statistics as the symbols or identification marks of various biological functions or drug effect of one or a Chinese herbal medicine and the metabolic products or the compound products; the bar code is used for identifying authentic Chinese herbal medicine and the patent traditional Chinese medicine of the component recipe and differentiating the Chinese traditional medicines of the same species in different production areas and Chinese traditional medicines of different species in different production areas; the bar code is used for instructing the control indexes of seed selection, area selection, growth or culture or plantation (cloning), collection and processing of the Chinese traditional medicines; and the bar code is used as the identification item of the quality standard of a Chinese traditional medicine and the metabolic product and as the indexes or model for separating the effective components and ingredients of the Chinese traditional medicine or the patent traditional Chinese medicine.

Description

(1) Technical field [0001] A high-throughput technical method based on modern molecular biology and cytology, using one or more cells isolated from various tissues and organs of the human body, animals, plants, or microorganisms, and captured by commercially available detection instruments The dynamic information integration of various cytology can be converted into a mathematical matrix representation by various statistical analysis methods, and can also be converted into a barcode representation composed of numbers or letters, endowing one or a type of traditional Chinese medicine and its metabolites or their compound products Symbolic markers of biological function. It is used to identify authentic Chinese medicinal materials and decoction pieces and Chinese patent medicines composed of them, to distinguish Chinese medicinal materials of the same species with different origins and different species with different origins, and to guide the selection, location, cultivation or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 柯越海傅惠英杨国良寿旗扬柯传奎
Owner 柯传奎
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