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Composite nanofiber membrane containing polysialic acid and preparation method and application

A technology of composite nanofibers and polysialic acid, which is applied in the field of tissue regeneration in biomedical engineering, can solve the problems of increased surgical risk, patient pain, and incomplete self-degradation, and achieves the improvement of kinematic function, high similarity, and promotion of The effect of nerve fiber repair

Active Publication Date: 2019-11-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional nerve repair scaffolds are mostly synthetic polymer materials. Although they have good biocompatibility, their self-degradation is not complete, and they need to be removed from the body through a second operation after the spinal cord trauma has healed, which increases the risk of surgery and the pain of patients.

Method used

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  • Composite nanofiber membrane containing polysialic acid and preparation method and application
  • Composite nanofiber membrane containing polysialic acid and preparation method and application
  • Composite nanofiber membrane containing polysialic acid and preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Weigh 1g of tetrabutylammonium bromide and dissolve it in 20ml of deionized water, add 1g of cation exchange resin (Dowex50WX2), stir at room temperature for 2h, collect the cation exchange resin by centrifugation, and wash 3 times with deionized water; then, weigh 200mg The polysialic acid (weight-average molecular weight is 5000Da) is dissolved in 20ml of deionized water, add the cation exchange resin collected above, and stir at room temperature for 2h, remove the cation exchange resin by centrifugation, take the supernatant, and wash the resin with deionized water for 3 The second time, the washing solution was combined into the supernatant, and freeze-dried to obtain tetrabutylammonium bromide-modified polysialic acid. The weight-average molecular weight was 8560 as measured by gel permeation chromatography.

Embodiment 2

[0024] Weigh 1g of tetrabutylammonium bromide and dissolve it in 20ml of deionized water, add 1g of cation exchange resin (Dowex50WX2), stir at room temperature for 2h, collect the cation exchange resin by centrifugation, and wash 3 times with deionized water; then, weigh 600mg The polysialic acid (weight-average molecular weight is 12000Da) is dissolved in 20ml of deionized water, add the cation exchange resin collected above, and stir at room temperature for 2h, remove the cation exchange resin by centrifugation, take the supernatant, and wash the resin with deionized water for 3 The second time, the washing solution was combined into the supernatant, and freeze-dried to obtain tetrabutylammonium bromide-modified polysialic acid. The weight-average molecular weight measured by gel permeation chromatography was 22400Da.

Embodiment 3

[0026] Weigh 1g of tetrabutylammonium bromide and dissolve it in 20ml of deionized water, add 1g of cation exchange resin (Dowex50WX2), stir at room temperature for 2h, collect the cation exchange resin by centrifugation, and wash 3 times with deionized water; then, weigh 950mg The polysialic acid (weight average molecular weight is 100000Da) is dissolved in the deionized water of 20ml, adds above-mentioned collected cation exchange resin, and stirs at room temperature 2h, removes cation exchange resin by centrifugation, takes supernatant, and washes resin 3 with deionized water The second time, the washing solution was combined into the supernatant, and freeze-dried to obtain tetrabutylammonium bromide-modified polysialic acid. The weight-average molecular weight was 185,000 Da as measured by gel permeation chromatography.

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Abstract

The invention provides a polysialic-acid-containing composite nanometer fibrous membrane. Polysialic acid and tetrabutylammonium bromide are subjected to cation exchange to obtain tetrabutylammonium bromide modified polysialic acid; then, a mixed solution containing polycaprolactone, methylprednisolone and tetrabutylammonium bromide modified polysialic acid is treated by a static spinning technology for preparing the polysialic-acid-containing composite nanometer fibrous membrane. The preparation method is convenient and fast; the repeatability is high; the obtained composite three-dimensional nanometer fibrous membrane can be prepared into a three-dimensional support frame to be applied to spinal cord injury treatment; the great secondary spinal cord injury of a spinal cord transaction model can be effectively inhibited; the nerve fiber regeneration and the kinematics function recovery are promoted.

Description

technical field [0001] The invention belongs to the field of tissue regeneration of biomedical engineering, and specifically relates to a polysialic acid-containing composite nanofiber membrane and a preparation method thereof, and the application of the polysialic acid-containing composite nanofiber membrane as a three-dimensional scaffold in the treatment of spinal cord injuries. Background technique [0002] Spinal cord injury refers to the transverse damage of the spinal cord due to mechanical trauma, tumor, inflammation and other reasons, which leads to obvious loss of movement and sensation below the injured surface of the limbs. In the United States, there are approximately 11,000 new cases of spinal cord injury each year, for a total of approximately 250,000 spinal cord injury patients. The incidence of spinal cord injury is increasing year by year, and the paraplegic and quadriplegic patients caused by spinal cord injury also bring a heavy burden to the family and s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18A61L27/20A61L27/50A61L27/54A61L27/56
CPCA61L27/18A61L27/20A61L27/50A61L27/54A61L27/56A61L2300/222A61L2300/232A61L2300/41A61L2300/412A61L2430/32C08L5/00C08L67/04
Inventor 杜永忠张硕王小娟应晓英
Owner ZHEJIANG UNIV
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