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Ferroferric oxide immunonanomagnetic bead, and preparation method and application thereof

A technology of ferric tetroxide and nano-magnetic beads, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of low saturation magnetization of nano-magnetic beads, achieve high saturation magnetization, increase preparation methods, and improve magnetic response. sexual effect

Inactive Publication Date: 2017-09-05
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the preparation process can only be carried out under the protection of nitrogen gas, which has certain limitations, and the prepared nano-magnetic beads have low saturation magnetization.

Method used

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  • Ferroferric oxide immunonanomagnetic bead, and preparation method and application thereof
  • Ferroferric oxide immunonanomagnetic bead, and preparation method and application thereof
  • Ferroferric oxide immunonanomagnetic bead, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] A kind of iron ferric oxide immune nano-magnetic beads and its preparation method and application, comprising the following steps:

[0069] (1) Preparation of nano magnetic beads:

[0070] ①Weigh 2.78g of FeSO 4 ·7H 2 O and 2.703 g FeCl 3 ·6H 2 O, be dissolved in the pure distilled water of 50ml, obtain iron salt solution; (FeSO 4 ·7H 2 The amount of O species is 2.78÷278.01≈0.01mol, Fe 2+ The molar concentration of FeCl is 0.2mol / L, FeCl 3 ·6H 2 The amount of O species is 2.703÷270.2962≈0.01mol, Fe 3+ The molar concentration is 0.2mol / L)

[0071] ② At a temperature of 65°C, first ultrasonically sonicate the iron salt solution for 6 minutes, then put the rotor on a magnetic stirrer and stir at high speed for 25 minutes, and at the same time add 160ml of 0.5mol / L sodium hydroxide solution dropwise, with the addition of NaOH , crystallization in the reaction liquid appeared dark red precipitate, and then stood for crystallization for 2 hours to obtain Fe 3 o 4...

Embodiment 2

[0094] A kind of iron ferric oxide immune nano-magnetic beads and its preparation method and application, comprising the following steps:

[0095] (1) Preparation of nano magnetic beads:

[0096] ①Weigh 2.78g of FeSO 4 ·7H 2 O and 5.406 g FeCl 3 ·6H 2 O, be dissolved in the pure distilled water of 50ml, obtain iron salt solution; (FeSO 4 ·7H 2 The amount of O species is 2.78÷278.01≈0.01mol, Fe 2+ The molar concentration of FeCl is 0.2mol / L, FeCl 3 ·6H 2 The amount of O species is 5.406÷270.2962≈0.02mol, Fe 3+ The molar concentration is 0.4mol / L)

[0097] ②At a temperature of 75°C, first ultrasonically sonicate the iron salt solution for 4 minutes, then put it into the S21-I constant temperature magnetic stirrer and stir it at high speed for 35 minutes, and at the same time add 180ml of 0.75mol / L sodium hydroxide solution dropwise, with the addition of NaOH Adding, the crystallization in the reaction liquid appears the dark red precipitate of black value, and then sta...

Embodiment 3

[0121] A kind of iron ferric oxide immune nano-magnetic beads and its preparation method and application, comprising the following steps:

[0122] (1) Preparation of nano magnetic beads:

[0123] ①Weigh 4.17g of FeSO 4 ·7H 2 O and 5.406 g FeCl 3 ·6H 2 O, be dissolved in the pure distilled water of 50ml, obtain iron salt solution; (FeSO 4 ·7H 2 The amount of O species is 4.17÷278.01≈0.015mol, Fe 2+ The molar concentration is 0.3mol / L, FeCl 3 ·6H 2 The amount of O species is 5.406÷270.2962≈0.02mol, Fe 3+ The molar concentration is 0.4mol / L)

[0124] ②At a temperature of 70°C, first ultrasonically sonicate the iron salt solution for 5 minutes, then put it into the S21-I constant temperature magnetic stirrer and stir at high speed for 30 minutes, and at the same time add 150ml of 1mol / L sodium hydroxide solution dropwise, with the addition of NaOH , crystallization in the reaction liquid appeared dark red precipitate, and then stood for crystallization for 2.5 hours to o...

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Abstract

The invention is a ferric iron tetroxide immune nanometer magnetic bead and its preparation method and application. A method for preparing ferric iron tetroxide immune nano magnetic beads, comprising the following steps: (1) preparing nano magnetic beads; (2) surface modification of nano magnetic beads; (3) preparation of immune magnetic beads. The invention also discloses the application of the ferric iron tetroxide immune nano magnetic beads. A ferroferric oxide immune nano-magnetic bead and its preparation method and application according to the present invention, the preparation method can not be limited by nitrogen, and the magnetic properties of Fe3O4 are improved; by improving the surface of Fe3O4 nano-magnetic beads It can be made into magnetic polymer microspheres, combined with nut allergic serum antibodies to make immune magnetic beads, increasing the types and preparation methods of immune nano magnetic beads; combining immune magnetic beads with ELISA without biosensor method , mass spectrometer and other expensive equipment with low cost can effectively detect nut allergenic proteins and improve the accuracy of prediction and evaluation of cross-allergic reactions.

Description

technical field [0001] The invention belongs to the technical field of immune nano magnetic beads, in particular to a Fe 3 o 4 Immunological nano magnetic beads and its preparation method and application. Background technique [0002] With the rapid development of the food industry, increasing environmental pollution, more and more allergic foods, and increasing incidence and prevalence of food allergy, food allergic diseases have become an emerging, public, and global health problem. Among them, allergies to nut products account for a large proportion. Because nut foods have extremely high nutritional value and unique flavor, they are deeply loved by consumers as therapeutic foods and snack foods. However, allergies to nut foods involve food 90% of allergies. Therefore, marking the existence of allergens on food labels is the most effective way to prevent allergic patients from ingesting potential allergens, and the detection of nut allergens is necessary. The existing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 张爱琳钟凯樊秀花张登科
Owner TIANJIN AGRICULTURE COLLEGE
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