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Method for performing large-scale and stable cultivation on phellinus linteus

A technology of Phellinus linteus and strains is applied in the field of large-scale and stable cultivation of Phellinus linteus strains, which can solve the problems of unstable quality and low survival rate, and achieve the effects of simple and easy operation, high survival rate and easy control.

Active Publication Date: 2017-09-08
金华市挺美科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for large-scale and stable cultivation of Phellinus fungi, which can solve the shortcomings of traditional methods such as low survival rate and unstable quality. The most important thing is to creatively apply the complex of zeolite powder and diatomaceous earth Pre-inoculation of bacteria provides a guarantee for the uniform distribution of bacteria in the medium and the uniform absorption of nutrients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for large-scale and stable cultivation of Phellinus igniarius strains, the specific process of cultivating Phellinus igniarius strains is as follows:

[0029] Mix 15kg of zeolite powder and 10kg of diatomaceous earth to prepare a composite, sterilize it at high temperature at 210°C, and then cool it with liquid nitrogen. After the temperature drops to 25°C, transfer to a sterile room to remove the Phellinus Connect to the complex; then take 5kg of mulberry sawdust, 3kg of glucose, 5kg of peptone, 2kg of potassium dihydrogen phosphate, 0.5kg of magnesium sulfate and 3kg of agar powder, mix with 81.5kg of water and stir evenly, then at a temperature of 121℃, Under the pressure of 0.1MPa, steam sterilize for 2 hours to obtain a sterile medium; then mix 25kg of the complex inoculated with Phellinus igniarius and the prepared 100kg sterile medium, then put it into a grid plate and encapsulate it with plastic , Put it into the culture room, keep it in the dark under the...

Embodiment 2

[0032] A method for large-scale and stable cultivation of Phellinus igniarius strains, the specific process of cultivating Phellinus igniarius strains is as follows:

[0033] Mix 12kg of zeolite powder and 8kg of diatomaceous earth to prepare a complex, sterilize at 200°C, and then cool with liquid nitrogen. After the temperature is reduced to 20°C, transfer to a sterile room to remove Phellinus igniarius Connect to the complex; then take 8kg of mulberry sawdust, 2kg of maltose, 4kg of soybean cake powder, 1kg of dipotassium hydrogen phosphate, 1kg of magnesium sulfate and 2kg of agar powder, mix with 10kg of water and stir evenly, then at a temperature of 121 ℃, pressure Sterilize it with steam for 2 hours under 0.1MPa to obtain a sterile culture medium; then mix 20kg of the complex inoculated with Phellinus linteus and the prepared 100kg sterile culture medium, put it into a grid plate, and encapsulate it with plastic. Put it into the culture room, keep it in the dark under the...

Embodiment 3

[0036] A method for large-scale and stable cultivation of Phellinus linteus strains, and the specific process of cultivating Phellinus linteus strains is as follows:

[0037] Mix 20kg of zeolite powder and 10kg of diatomaceous earth to prepare a complex, sterilize at 220°C, and then cool with liquid nitrogen. After the temperature is reduced to 25°C, transfer to a sterile room to remove Phellinus igniarius strains Connect to the complex; then take 7kg of mulberry sawdust, 3kg of sucrose, 5kg of bran, 1kg of potassium dihydrogen phosphate, 0.5kg of magnesium sulfate and 3kg of agar powder, mix with 8.5kg of water and stir evenly, then at a temperature of 121℃ , The pressure is 0.1MPa, steam sterilization for 2h to obtain a sterile medium; then 30kg of the complex inoculated with Phellinus igniarius is mixed with the prepared 100kg sterile medium, and then the grid plate is filled with plastic Encapsulate, put it in a culture room, and keep it in a dark state under the conditions o...

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PUM

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Abstract

The invention belongs to the technical field of phellinus linteus cultivation, and provides a method for performing large-scale and stable cultivation on phellinus linteus. The method comprises the following steps: compounding zeolite powder and diatomite, performing high-temperature sterilization, rapidly cooling to a certain temperature by utilizing liquid nitrogen, and inoculating the phellinus linteus into a complexing body of the zeolite powder and the diatomite in a sterile room; prefabricating a culture medium by using water, mulberry sawdust, a carbon source, a nitrogen source, a phosphorus fertilizer, a potassium fertilizer, magnesium sulfate and agar powder, and performing sterilization by using high-pressure steam; mixing the complexing body inoculated with the phellinus linteus and a nutritional medium according to a proportion, putting the mixture into a grid plate, packaging by using plastic, putting into a culture room, controlling the temperature and the relative humidity of the culture room, maintaining a dark state, and cultivating for a certain time to obtain uniform mycelium. When the method is used for cultivating the phellinus linteus, the survival rate is high, the quality is stable and large-scale cultivation can be realized.

Description

Technical field [0001] The invention relates to the technical field of Phellinus igniarius strain cultivation, in particular to a method for large-scale and stable cultivation of Phellinus igniarius strain. Background technique [0002] Phellinus igniarius is a precious medicinal fungus, known as "forest gold". Its fruit body is used as medicine to treat gynecological diseases such as bleeding, hemorrhage and amenorrhea. Its anti-cancer, anti-bacterial, anti-oxidant and anti-fibrosis effects are also very significant, so it has become a research hotspot in recent years. Japan and South Korea have conducted more and more in-depth studies on Phellinus sibiricum. Since wild Phellinus igniarius has extremely strict requirements on the growth environment and requires a growth cycle of 20 to 30 years, it is extremely rare, in short supply, and very expensive. Therefore, artificial cultivation of Phellinus linteus, which can be used as a raw material for medicine, is of great necessit...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 陈庆王镭迪曾军堂
Owner 金华市挺美科技有限公司
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