Biocontrol actinomycetes strain for controlling pepper phytophthora blight, and application of strain

A technology of pepper blight and actinomycetes, applied in the direction of application, bacteria, and biocides, can solve problems such as unsatisfactory chemical control effects and environmental pollution, achieve good development and application prospects, reduce farmland pollution, and effectively prevent and control pepper blight Effect

Active Publication Date: 2017-09-08
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a biocontrol actinomycete and its application method for the effective prevention and treatment of pepper blight in view of the serious

Method used

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  • Biocontrol actinomycetes strain for controlling pepper phytophthora blight, and application of strain
  • Biocontrol actinomycetes strain for controlling pepper phytophthora blight, and application of strain
  • Biocontrol actinomycetes strain for controlling pepper phytophthora blight, and application of strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation of Antagonistic Actinomycetes

[0027] The specific process is as follows:

[0028] 1. Collection of soil samples

[0029] The rhizosphere soil at a depth of 5-10 cm from different hot pepper plots was collected from Kunkou Village, Dongfeng Town, Jian'ou City, Fujian Province, packed and marked, brought back to the laboratory, and separated after natural air-drying.

[0030] 2. Isolation of actinomycetes

[0031] Separation was performed using the plate dilution method. Weigh 10 g of the soil sample, pour it into a conical flask filled with small glass beads and 90 ml of sterile water, shake it for 30 min, then let it stand for 5 minutes, dilute it by 10 times successively, and prepare 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Suspension of different concentrations, each 0.1 ml of the suspension was drawn and added to Gao's No. 1 medium (add K with a final concentration of 50 mg / L 2 Cr 2 o 7 ) plate, spread evenly and culture at 28°C for observati...

Embodiment 2

[0032] Identification of embodiment two bacterial strain St19

[0033] 1. Observation of morphological characteristics

[0034] The strain St19 was streak-inoculated on Gao's No. 1 medium, inserted into a sterile cover glass at 45 °C obliquely, and cultured at 28 °C for 10 days, then the cover glass was taken out to observe the morphological characteristics of the hyphae under an optical microscope and an electron microscope ( figure 1 with figure 2 ).

[0035] 2. Observation of culture characteristics

[0036] Using the 8 kinds of media recommended in the Streptomyces Identification Manual, culture the strain St19 at 28 °C for 7-10 days, observe the growth of the bacteria, and record the color of the aerial hyphae and basal hyphae and whether soluble pigments are produced. Strain St19 grew well on Gao's No. 1 medium, Chabek medium, starch ammonium medium, PDA medium, glucose yeast extract medium and oat medium, and the hyphae and aerial hyphae in the substrate developed ...

Embodiment 3

[0041] The optimization of embodiment three St19 bacterial strain fermentation technology

[0042]The above strains were inoculated in Gaoshi No. 1 liquid medium, cultured at 28 °C and 200 r / min for 2 days, then made into seed liquid, and inoculated in the fermentation medium. Taking Phytophthora capsici as the target bacteria, through the screening of carbon source and nitrogen source, single factor test and orthogonal test of nutritional conditions, the optimal fermentation formula of this strain is obtained: soluble starch 2%wt, glucose 1.5%wt, Soybean flour 2%wt, MgSO 4 ·7H 2 O 0.05%wt, K 2 HPO 4 0.1%wt, NaCl 0.1%wt, CaCO 3 0.2%wt, the rest is distilled water. The optimal fermentation conditions were: inoculum volume 6% vol, bottle volume 70 mL / 250 mL, initial pH value of fermentation medium 7.0, 28°C, 200 r / min, fermentation time 5 days, and a concentration of 10 8 CFU / ml of St19 strain fermentation broth. After the fermentation broth was centrifuged at 4000 r / mi...

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Abstract

The invention discloses a biocontrol actinomycetes strain for controlling pepper phytophthora blight, and application method of the strain, and belongs to the technical field of crop disease control. The biocontrol actinomycetes strain is streptomyceseuropaeiscabiei St19, and is registered and preserved in China General Microbiological Culture Collection Center (CGMCC) on March 24, 2014; and the preservation number is CGMCCNO.8962. The strain generates an active substance to inhibit growth of phytophthora capsici, and represents a better control effect on the pepper phytophthora blight. The strain can be applied to root irrigation in a seedling stage or an adult-plant stage of pepper. An inoculant can also be mixed with an organic fertilizer to prepare a microbial fertilizer. The strain St19 is obtained from pepper rhizosphere soil, is harmonious and compatible with soil ecology, and is non-toxic and non-virulent, so that the strain has a good application prospect in biological control of the pepper phytophthora blight.

Description

technical field [0001] The invention relates to a biocontrol actinomycete strain for preventing and treating pepper blight and an application thereof, belonging to the technical field of crop disease prevention and control. Background technique [0002] Phytophthora capsici (Phytophthora capsici) The pepper blight caused by it is a devastating soil-borne disease in pepper production, which currently occurs in all pepper producing areas in the world. The disease can not only spread in the soil through rain or irrigation water, causing a large area of ​​dead seedlings, but also spread through airflow, causing damage to pepper stems, leaves, flowers, and fruits. It can cause the failure of host crops, seriously affect the development of my country's pepper industry, and cause major economic losses. [0003] At present, the prevention and control of pepper blight mainly depends on the cultivation of disease-resistant varieties and the use of chemical agents. Among them, the cul...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C05G3/00C12R1/465
CPCA01N63/10C05G3/00C12N1/20C12N1/205C12R2001/465
Inventor 李本金陈庆河刘裴清王荣波翁启勇
Owner INST OF PLANT PROTECTION FAAS
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