Preparation and applications of soil improvement agent trichoderma atroviride DQ1 composite powder TLB

A soil improver, Trichoderma dark green technology, applied in the soil improver Trichoderma dark green DQ1 composite powder TLB and its application field, can solve the problems of human and animal toxicity, environmental pollution, residue, etc., to achieve the promotion of crop growth, improvement Soil, fast occupying effect

Inactive Publication Date: 2017-09-15
刘铜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] An object of the present invention is to provide soil improver Trichoderma dark green DQ1 composite powder TLB, this soil improver Trichoderma dark green DQ1 composite powder TLB solves the chemical pesticide currently used when preventing and treating crop ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] This soil improver Trichoderma dark green DQ1 composite powder TLB is prepared by the following preparation method:

[0018] Step 1: Inoculate the Trichoderma dark green DQ1 strain on the PDA slant medium, culture at 28°C for 72 hours, wash the conidia with sterilized distilled water to make a spore liquid, and the number of spores in the spore liquid is 10 6 1 / mL, then put it into the sterilized PD culture medium at 1% (volume ratio), put it in 28℃, 120rpm and cultivate for 36h;

[0019] Step 2: Mix bran, diatomaceous earth, rice husk, and water in a ratio of 2:1:1:4 (mass ratio), stir evenly, and put it into a 200mL plastic bottle, the amount of which is 1% of the height of the plastic bottle. / 2, and then sterilized under high pressure at 121°C for 20 minutes. After cooling, put it into a plastic bottle according to the inoculation amount of 1%, shake it up and down evenly, and culture it at a temperature of 28°C and a humidity of 80% for 6 days; the fermented bacter...

Embodiment 2

[0024] This soil improver Trichoderma dark green DQ1 composite powder TLB is prepared by the following preparation method:

[0025] Step 1: Inoculate the Trichoderma dark green DQ1 strain on the PDA slant medium, culture at 28°C for 96 h, wash the conidia with sterilized distilled water, and make a spore liquid, the number of spores in the spore liquid is 10 6 1 / mL, then put it into the sterilized PD culture medium at 1% (volume ratio), put it in 28°C, 120rpm for 40h;

[0026] Step 2: Mix bran, diatomaceous earth, rice husk, and water in a ratio of 2:1:1:4 (mass ratio), stir evenly, and put it into a 200mL plastic bottle, the amount of which is 1% of the height of the plastic bottle. / 2, and then sterilized under high pressure at 121°C for 20 min. After cooling, put it into a plastic bottle according to the inoculum amount of 1%, shake it up and down evenly, and culture it at a temperature of 28°C and a humidity of 80% for 7 days; the fermented bacteria in the plastic bottle ...

Embodiment 3

[0031] This soil improver Trichoderma dark green DQ1 composite powder TLB is prepared by the following preparation method:

[0032] Step 1: inoculate the Trichoderma dark green DQ1 strain on the PDA slant medium, culture at 28°C for 84 h, wash the conidia with sterilized distilled water, and make a spore liquid, the number of spores in the spore liquid is 10 61 / mL, then put it into the sterilized PD culture medium at 1% (volume ratio), put it in 28°C, 120rpm for 48h;

[0033] Step 2: Mix bran, diatomaceous earth, rice husk, and water in a ratio of 2:1:1:4 (mass ratio), stir evenly, and put it into a 200mL plastic bottle, the amount of which is 1% of the height of the plastic bottle. / 2, and then sterilized under high pressure at 121°C for 20 min. After cooling, put it into a plastic bottle according to the inoculum amount of 1%, shake it up and down evenly, and culture it at a temperature of 28°C and a humidity of 80% for 7 days; the fermented bacteria in the plastic bottle S...

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PUM

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Abstract

The invention relates to a soil improvement agent trichoderma atroviride DQ1 composite powder TLB, and applications thereof. The soil improvement agent trichoderma atroviride DQ1 composite powder TLB is prepared via following steps: 1, PDA slant culture-medium is inoculated with trichoderma atroviride DQ1 so as to obtain a spore liquid, and sterilized PD culture medium is inoculated with the spore liquid for culturing; 2, a pore suspension liquid is prepared from wheat bran, diatomite, rice husk, and water, and is taken as a solid shallow tray fermentation seed liquid; 3, a solid shallow tray fermentation medium is prepared from wheat bran, diatomite, streptomycin, and sterile water at a mass ratio of 50:25:1:50, is stirred, and is sterilized, the sterilized solid shallow tray fermentation medium is mixed with the solid shallow tray fermentation seed liquid, culturing is carried out, an obtained shallow tray fermentation product is subjected to air drying and smashing so as to obtain trichoderma atroviride DQ1 spore powder; and 4, the soil improvement agent trichoderma atroviride DQ1 composite powder TLB is prepared. The soil improvement agent trichoderma atroviride DQ1 composite powder TLB is capable of preventing and treating vegetable soil borne diseases, and is harmless for human and animals, and no environment pollution is caused.

Description

1. Technical field [0001] The invention relates to a biological bacterial agent for preventing and controlling crop diseases, in particular to a soil improver Trichoderma dark green DQ1 composite powder TLB and an application thereof. 2. Background technology [0002] With the growth of crop planting years, the planting pattern is relatively fixed, resulting in the accumulation of a large number of harmful organisms in the soil, and the occurrence of diseases is becoming more and more serious. In addition, the continuous cultivation of the soil has caused obvious changes in the physical properties of the soil, and a large amount of compaction of the soil. Therefore, soil-borne diseases and soil fertility decline have become important limiting factors affecting crop production. However, the current prevention and control of crop diseases mainly relies on chemical means. As we all know, chemical pesticides have residues in crops, air, water and soil, causing excessive pesticid...

Claims

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Application Information

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IPC IPC(8): C09K17/40A01N63/04A01N61/00A01P5/00A01P3/00C12N1/14C12R1/885C09K101/00
CPCA01N61/00A01N63/30C09K17/40C09K2101/00C12N1/14A01N2300/00
Inventor 刘铜侯巨梅
Owner 刘铜
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