Method for identifying Escherichia coli serotype and detecting Escherichia coli virulence factor of piglets

Abstract

The invention discloses a method for identifying Escherichia coli serotype and detecting an Escherichia coli virulence factor of piglets and belongs to the technical field of piglet breeding and disease resistance. The method comprises the following steps: performing bacteria separation and purification, performing biochemical identification on a to-be-detected strain, identifying Escherichia coli O serotype, identifying Escherichia coli O antigen typing serotype and detecting the Escherichia coli virulence factor. The 64 separated piglet pathogenic Escherichia coli strains are subjected to serotype identification and virulence factor gene PCR (Polymerase Chain Reaction) detection, and the results show that the piglet Escherichia coli strains have complicated serotypes, regional distribution is wide, the difference is great, and data reference is provided for screening predominant serotypes in the region and preparing polyvalent vaccines from strains with good immunogenicity.

Description

Technical field [0001] The invention belongs to the field of pig breeding and disease -prevention technology, which involves a kind of child's colonbaccinobacterium serum identification and its toxic force factor detection method. Background technique [0002] Pig diarrhea is one of the most common diseases in pig breeding. It often leads to slow growth, stagnation and even death of piglets, causing great economic losses to the pig industry. The causes of Chinese piglet diarrhea are complicated, mainly infectious diarrhea. Among them, the original infectious gastroenteritis virus, epidemic diarrhea virus, and rotary virus of viral diseases; bacterial diseases mainly include E. coli, Salmonella, and Weishalotus Wait. [0003] Piglandic E. coli is a type of infectious disease caused by pathogenic E. coli, causing piglets to yellow, white diarrhea, and edema. The piglets of the piglets are the acute and mortality infectious diseases of the newborn piglets. They mainly occur during t...

AI Technical Summary

Problems solved by technology

The diversity of serotypes of Escherichia coli antigens has caused great difficulties in the prevention and treatment of Escherichia coli disease. Although some vaccines are currently used to prevent them, the effect is not ideal

[0004] At present, the routine detection method of porcine E. coli toxin is time-consuming, expensive and complicated, and is not suitable for the detection of large quantities of clinical isolates

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