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Ultra-rapid detection of helicobacter pylori in gastric mucosal biopsies

A technology for Helicobacter pylori and gastric mucosa, which is applied in the directions of microorganisms, pH value testing, microorganism-based methods, etc., can solve the problems of non-guarantee, reduce test sensitivity, etc., and achieve the effect of high stability

Active Publication Date: 2017-10-17
马可卡诺尼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the test also has certain limitations: in addition to a high sensitivity (100%) within a 5-minute reaction time for high to moderate bacterial loads, only 55% of the samples tested for low bacterial loads Responds within 5 minutes and 90% changes color within 30 minutes
Also, the use of two different indicators does not always provide easy-to-read results for slight color changes at intermediate pH values ​​(7.0 to 7.9) close to the pKa of the reactants, sometimes in cases of mild H. pylori infection interpretations that cast doubt on the results
Furthermore, 10% urea does not guarantee a sufficiently acidic pH for long-term storage of diagnostic reagents
[0032] It should also be considered that, in recent years, widespread use of generic antibiotic therapy has increased the detection of low H. pylori loads, which further reduces the sensitivity of the test

Method used

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Examples

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Effect test

Embodiment 2

[0091] Example 2 - Clinical study by using a diagnostic composition containing 10% urea in deionized water at pH 6.8; 1% bromothymol blue and 2% phenol red.

[0092] By using a diagnostic composition as described in patent application ITRM20030399, which contains 10% urea in deionized water at pH 6.8; 1% bromothymol blue and 2% phenol red, the histological data were compared. An overall sensitivity of 88% was obtained, and histological data were accepted as the standard reference for diagnosing the presence of bacteria. In this case, the study enrolled 86 patients who were not treated with antisecretory protective drugs; of these 86 patients, 29 turned out to be H. pylori free and 57 turned out to be infected. Of these 57 infected biopsies, 7 had a high bacterial load, 27 had a moderate load, and 23 had a low level of infection. Of the 23 biopsies with extremely low bacterial loads, 7 failed to react in a diagnostic test using a composition containing 10% urea in deionized at...

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Abstract

Composition and relative kit to quickly detect the presence of Helicobacter Pylori in biopsy specimens of the gastric mucosa during the gastroscopy analysis comprising 5% urea and 0.5-10% of bromothymol blue in saline solution and having pH equal to 4.8.

Description

technical field [0001] The present invention relates to a diagnostic composition in the form of an aqueous solution for the extremely rapid detection of the presence of Helicobacter Pylori in biopsy samples taken during endoscopic analysis and for the evaluation of their bacterial load. Background technique [0002] Some gastrointestinal diseases (such as gastric ulcer or duodenal ulcer, especially active chronic gastritis) are closely related to partial colonization or diffuse colonization of gastric mucosa by Helicobacter pylori. This bacterium, also known as Campylobacter Pylori, degrades urea via urease. The presence of Helicobacter pylori in biopsy fragments of human gastric mucosa has been known for decades (Freedberg A.S. and Barron L.E. "The presence of spirochetes in human gastric mucosa", 1940, Am. J.Dig.Dis., 7:443-5; Marshall B.J. "Unidentified Curved bacilli on Gastric epithelium in active chronic gastritis" (Unidentified Curved bacilli on Gastric epithelium in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12Q1/58C12R1/01
CPCC12Q1/04C12Q1/58C12Q1/06G01N31/221
Inventor 马可·卡诺尼V·伊奥文
Owner 马可卡诺尼
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