Ultra-rapid detection of Helicobacter pylori in gastric mucosal biopsies

A technology of Helicobacter pylori and gastric mucosa, applied in the direction of microorganisms, testing pH value, and methods based on microorganisms, can solve the problems of reducing test sensitivity and achieve high stability

Active Publication Date: 2021-03-12
马可卡诺尼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0034] It should also be considered that, in recent years, widespread use of generic antibiotic therapy has increased the detection of low H. pylori loads, which further reduces the sensitivity of the test

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0093] Example 2 - Clinical study by using a diagnostic composition containing 10% urea in deionized water at pH 6.8; 1% bromothymol blue and 2% phenol red.

[0094] By using a diagnostic composition as described in patent application ITRM20030399, which contains 10% urea in deionized water at pH 6.8; 1% bromothymol blue and 2% phenol red, the histological data were compared. An overall sensitivity of 88% was obtained, and histological data were accepted as the standard reference for diagnosing the presence of bacteria. In this case, the study enrolled 86 patients who were not treated with antisecretory protective drugs; of these 86 patients, 29 turned out to be H. pylori free and 57 turned out to be infected. Of these 57 infected biopsies, 7 had a high bacterial load, 27 had a moderate load, and 23 had a low level of infection. Of the 23 biopsies with extremely low bacterial loads, 7 failed to react in a diagnostic test using a composition containing 10% urea in deionized at...

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PUM

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Abstract

Composition for rapid detection of the presence of Helicobacter pylori in gastric mucosal biopsy specimens during gastroscopic analysis and related kits comprising 5% urea and 0.5‑10% bromothymol in saline solution at pH equal to 4.8 blue.

Description

technical field [0001] The present invention relates to a diagnostic composition in the form of an aqueous solution for the extremely rapid detection of the presence of Helicobacter Pylori in biopsy samples taken during endoscopic analysis and for the evaluation of their bacterial load. Background technique [0002] Some gastrointestinal diseases (such as gastric ulcer or duodenal ulcer, especially active chronic gastritis) are closely related to partial colonization or diffuse colonization of gastric mucosa by Helicobacter pylori. This bacterium, also known as Campylobacter Pylori, degrades urea via urease. The presence of Helicobacter pylori in biopsy fragments of human gastric mucosa has been known for decades (Freedberg A.S. and Barron L.E. "The presence of spirochetes in human gastric mucosa", 1940, Am. J.Dig.Dis., 7:443-5; Marshall B.J. "Unidentified Curved bacilli on Gastric epithelium in active chronic gastritis" (Unidentified Curved bacilli on Gastric epithelium in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12Q1/58C12R1/01
CPCC12Q1/04C12Q1/58C12Q1/06G01N31/221
Inventor 马可·卡诺尼V·伊奥文
Owner 马可卡诺尼
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