The invention discloses a gastroscope mucilage compound preparation. The gastroscope mucilage compound preparation comprises the following raw materials according to weight ratio in grams: 0.005-0.01 part of chymotrypsin, 0.05-0.1 part of a sodium bicarbonate powder and 0.615-1.23 parts of a simethicone powder. A preparing method of the gastroscope mucilage compound preparation comprises the following steps: according to the weight ratio, filling the 0.005-0.01 part of chymotrypsin into a bag A, filling the 0.05-0.1 part of the sodium bicarbonate powder into a bag B, filling the 0.615-1.23 parts of the simethicone powder into a bag C, finally, respectively filling the bag A, the bag B and the bag C into a packaging box. The use method is as follows: firstly, one box of the product is fetched five to ten minutes before gastroscope examinations, the bag A, bag B and bag C preparations in the box are respectively poured into a small scale cup, and warm boiled water is added to 50ml; then, the solution is agitated until the chymotrypsin, the sodium bicarbonate and the simethicone powder are fully dissolved, and the solution can be taken. Therefore, the preparation achieves defoaming and lubricating functions, and can effectively decompose gastric juice, clarify the gastric juice and effectively improve the definition of a gastroscope.