A method for establishing a three-dimensional skin model for anti-inflammatory and anti-allergic efficacy evaluation

Abstract

A method for establishing a three-dimensional skin model of the present invention comprises the following steps: 1) preparing an extracellular matrix polymer hydrogel solution; 2) mixing fibroblasts with the extracellular matrix polymer hydrogel solution, and putting them into a culture chamber Cultivate; 3) Inoculate keratinocytes and culture through air-liquid interface to obtain a three-dimensional skin model including dermis, epidermis and stratum corneum. The method of the invention can effectively establish a three-dimensional skin model, and the preparation method is simple and convenient, can be used for screening skin anti-inflammatory and anti-sensitivity drugs, and has excellent application prospects.

Description

technical field [0001] The present invention relates to a method for establishing a skin model, in particular to a method for establishing a novel three-dimensional skin model for evaluating anti-inflammatory and anti-allergic effects. Background technique [0002] As an important tissue and organ that covers and protects the body surface, the skin is the first barrier to block the interference of external substances. It is easily damaged by factors such as trauma, burns, inflammation, ulcers, postoperative tumors, and congenital diseases. At the same time, it will pass some special reactions to defend. The incidence of allergic skin diseases such as dermatitis and eczema in the whole world exceeds 10%. At present, it is very inconvenient and costly to screen anti-inflammatory and anti-allergic drugs or cosmetic raw materials for skin, ear swelling of mice induced by xylene, swelling of paws of rats induced by egg white, chronic inflammation model of cotton ball granuloma i...

AI Technical Summary

Problems solved by technology

At present, it is very inconvenient and costly to screen anti-inflammatory and anti-allergic drugs or cosmetic raw materials for skin, ear swelling of mice induced by xylene, swelling of paws of rats induced by egg white, chronic inflammation model of cotton ball granuloma in rats, small Rat local lymph node sensitization experiment is a commonly used animal experimental model

However, with European cosmetics regulations promulgating the ban on animal experiments in cosmetic products, the screening and development of anti-inflammatory and anti-allergic chemicals and cosmetic raw materials is more difficult to achieve

At the same time, due to the complexity of skin sensitization and inflammation mechanisms, it is difficult to fully reflect the entire sensitization and inflammation process in the same in vitro test system. Efficacy provides prediction and assessment

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