Pipeline for culturing cells in vitro

Abstract

The invention discloses a pipeline which comprises a first pipe, a second pipe, a third pipe and a fourth pipe. The first end of the first pipe is provided with a plurality of first input branches. Each first input branch is provided with a pipe pressure valve; the first end of the second pipe is connected with a peristaltic pump, the peristaltic pump is connected to a separation culture tank through the third pipe; the first end of the fourth pipe is provided with a plurality of second input branches, and each second input branch is provided with a pipe pressure valve; the second end of the first pipe, the second end of the second pipe and the second end of the fourth pipe are communicated. When the pipeline is applied, each first input branch and each second input branch are connected to a containing bag of a material to be input into the separation culture tank, and then the peristaltic pump is opened and the corresponding pipe pressure valve are turned on as needed to add the needed material into the separation culture tank. The pipeline can convey the material for being added into the separation culture tank, the material makes no contact with the external environment in the input process, and the phenomenon that cells inside the separation culture tank is polluted in the adding process can be avoided.

Description

technical field [0001] The invention relates to the technical field of machinery industry, and more specifically, relates to a pipeline for cell culture in vitro. Background technique [0002] MSC (mesenchymal stem cells, mesenchymal stem cells) cells, NK (natural killer cell, natural killer cells), CAR-T (Chimeric Antigen Receptor T-Cell, chimeric antigen receptor T-cell) cells, etc. need to use separation culture tanks However, the cells need to be separated and added to the culture tank many times during the culture tank process, which increases the risk of contamination of the cells during the culture process. [0003] Therefore, how to make the cells polluted by adding liquid etc. into the separation culture tank when cultured in vitro by using the separation culture tank is a technical problem to be solved urgently by those skilled in the art. Contents of the invention [0004] In view of this, the present invention provides a pipeline with a peristaltic pump, which...

AI Technical Summary

Problems solved by technology

[0002] MSC (mesenchymal stem cells, mesenchymal stem cells) cells, NK (natural killer cell, natural killer cells), CAR-T (Chimeric Antigen Receptor T-Cell, chimeric antigen receptor T-cell) cells, etc. need to use separation culture tanks For culturing, however, the cells need to be separated several times during the culture tank to add liquid to the culture tank, which increases the risk of contamination of the cells during the culture process

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