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Streptococcus agalactiae plasmid for expressing fluorescent protein and construction method and application thereof

A technology of streptococcus lactis and fluorescent protein, which is applied in the field of streptococcus agalactiae plasmid and its construction, can solve the problem of not expressing fish-derived streptococcus agalactiae efficiently, and achieve the effect of saving experiment cycle, reducing experiment cost and simple method

Active Publication Date: 2017-11-10
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

However, at present, there is no relevant report on the red fluorescent protein-labeled strain of fish-derived Streptococcus agalactiae at home and abroad, and there is no reference method for high-efficiency expression of red fluorescent protein-labeled fish-derived Streptococcus agalactiae

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  • Streptococcus agalactiae plasmid for expressing fluorescent protein and construction method and application thereof
  • Streptococcus agalactiae plasmid for expressing fluorescent protein and construction method and application thereof
  • Streptococcus agalactiae plasmid for expressing fluorescent protein and construction method and application thereof

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Embodiment Construction

[0032] The following description is a preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications are also considered Be the protection scope of the present invention. In the following specific embodiments, if the experimental methods of specific conditions are not indicated, generally follow the conventional methods and conditions of molecular biology within the skill of the art, and such techniques and conditions are fully explained in the literature.

[0033] The technical solutions of the present invention will be described in detail below in conjunction with the accompanying drawings and specific implementation methods; obviously, the described embodiments are part of the present invention, not all of them. Both the tilapia-derived Streptococcus agalactiae virulent strai...

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Abstract

The invention belongs to the technical field of biological monitoring, and particularly relates to a streptococcus agalactiae plasmid for expressing fluorescent protein and a construction method and application thereof. The construction method comprises the steps of integrating a promoter of the fish-source streptococcus agalactiae recA gene into the upstream of the mcherry gene through the overlap PCR method, then connecting the fusion fragment to a PBCH carrier, and obtaining the pBCH-RM plasmid. By application of the pBCH-RM plasmid to strains of the fish-source streptococcus agalactiae, red fluorescent protein can be effectively and stably expressed, and the strong toxicity biological characteristics of parent strains are retained; the streptococcus agalactiae plasmid for expressing fluorescent protein can be applied to the basic researches of the route of transmission, fluorescence tracing, interaction between pathogen and host and the like in the streptococcus agalactiae.

Description

technical field [0001] The invention belongs to the technical field of biological monitoring, and in particular relates to a Streptococcus agalactiae plasmid for expressing fluorescent protein and its construction method and application. Background technique [0002] As one of the excellent freshwater cultured species, Tilapia has been artificially cultured on a large scale in southern my country such as Guangdong, Guangxi and Hainan. According to statistics from the Fisheries Bureau of the Ministry of Agriculture, the tilapia aquaculture output in my country was about 178 in 2015. million tons (2016 China Fishery Statistical Yearbook), the total output ranks first in the world. However, streptococcal disease has seriously restricted the healthy and sustainable development of tilapia industry in my country in recent years. Since 2009, streptococcus outbreaks have occurred in cultured tilapia in many regions of my country, with a cumulative mortality rate of 30% to 80%, and m...

Claims

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Application Information

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IPC IPC(8): C12N15/74C12N15/66C12N15/65C12N1/21C12R1/46
CPCC07K14/43595C12N15/746C12N2830/34
Inventor 张德锋可小丽卢迈新刘志刚曹建萌王淼衣萌萌刘春
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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