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Beauveria bassiana BbL25 strain for preventing and treating solenopsis invicta and application thereof

A technology of Beauveria bassiana and RIFA, which is applied in the fields of application, fungi, and methods based on microorganisms, which can solve the unproven control effect of RIFA and the unsatisfactory effect of controlling RIFA by pathogenic insect-borne fungi and other problems, to achieve good field control effects and great application prospects

Active Publication Date: 2017-12-01
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the effect of the above-mentioned pathogenic fungi on preventing and controlling RIFA is not satisfactory, and the researches are only carried out under indoor conditions. Because the indoor measurement conditions are different from the actual application, environmental factors such as temperature and humidity have a great influence on the pathogenicity of white deadlock. (Liang Zongqi and Ye Yuchang, 1994; Pan Zhiping, 2008), so the control effect of these entomogenic fungi on RIFA in field environment has not been confirmed

Method used

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  • Beauveria bassiana BbL25 strain for preventing and treating solenopsis invicta and application thereof
  • Beauveria bassiana BbL25 strain for preventing and treating solenopsis invicta and application thereof
  • Beauveria bassiana BbL25 strain for preventing and treating solenopsis invicta and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation and identification of Beauveria bassiana BbL25 bacterial strain

[0037] 1. Strain isolation

[0038] The strain is isolated and purified from the soil of Xilamulun, Balin Right Banner, Chifeng City, Inner Mongolia.

[0039] 2. Morphological identification of strains

[0040] The obtained strain was cultured on potato glucose medium, and its morphological characteristics were as follows: the colony was white at the beginning, and the surface was fluffy; on the 12th day of the colony culture, the colony was round, the center and the edge were slightly raised, and most of the middle part was slightly depressed , the surface is white, fluffy, the back is light yellow, the diameter is (68.16±1.96) mm, and the middle part of the colony becomes powdery in the later stage (such as figure 1 shown). Under the optical microscope, the hyphae are septate, branched, transparent, and the width is (1.08±0.14) μm; Spores grow densely on conidiophores; conidia ar...

Embodiment 2

[0047] Example 2 Beauveria bassiana BbL25 bacterial strain is to the toxicity determination of red fire ants

[0048] 1. Preparation of spore suspension

[0049] (1) Conidia production

[0050] Take the slant strain of Beauveria bassiana BbL25 bacterial strain preserved in Example 1, scrape a small amount of conidia and inoculate them on a PDA plate, cultivate them in a 25° C. The spores are ready for use.

[0051] (2) Preparation of molecular spore suspension

[0052] Place the conidia scraped in step (1) into a centrifuge tube, add 0.02% Tween-80 solution, shake and shake well, measure the concentration of spores with a hemocytometer, and prepare conidia suspensions of different concentrations required liquid, spare.

[0053] Conidia suspensions of Beauveria bassiana BbL25 strain were prepared at the following concentrations:

[0054] (1) 1×10 6 a / mL;

[0055] (2)5×10 5 a / mL;

[0056] (3) 1×10 5 a / mL;

[0057] (4)5×10 4 a / mL;

[0058] Under laboratory conditions...

Embodiment 3

[0070] Example 3 Several kinds of entomogenous fungi are tested for the pathogenicity of red imported fire ants

[0071] 1. According to the method for producing conidia in step (1) of the above-mentioned embodiment 2, code-named MS-01, MS-02, MS-03, MS-05, MS-06, MS- 08, MS-13, MS-22, L18, BbL25 strains and Java strains isolated from Spodoptera litura worms in Guangzhou area, and Corynesium fumigatus (IfB01) isolated from Bemisia tabaci worms in Guangzhou area were prepared to 1.0 ×10 7 spores / mL. Under laboratory conditions, the RIFA was treated by immersion with spore suspension, and the mortality rate of RIFA was investigated for 14 consecutive days after treatment.

[0072] Mortality (%) = number of dead insects / number of insects before treatment × 100%

[0073] Corrected mortality rate (%)=(treatment mortality rate-control mortality rate) / (1-control mortality rate)×100%

[0074]2. The corrected mortality of different strains to Bemisia tabaci is shown in Table 3; the ...

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Abstract

The invention discloses a Beauveria bassiana BbL25 strain for preventing and treating solenopsis invicta. The strain is preserved at China Center for Type Culture Collection on February 16, 2017 and the preservation number is CCTCC NO: M2017045. The Beauveria bassiana BbL25 strain is highly pathogenic to the solenopsis invicta, and half lethal concentration (LC50) to the solenopsis invicta is 8.15x10<5> conidium / mL on the 14th day after inoculation, and the strain has a good field controlling effect on the solenopsis invicta; the controlling effect of 1x10<7> / mL condinal suspension on the solenopsis invicta reaches 82.52% on the 14th day after drug delivering, and the effect is same as chemical insecticide 0.25% fluoxane. The Beauveria bassiana BbL25 strain can be used as a living biological insecticide and has no pollution to the environment and is non-toxic to human beings and animals and is suitable for the production of organic agricultural products, and great application prospects are achieved.

Description

technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to a strain of Beauveria bassiana BbL25 for controlling red fire ants and its application. Background technique [0002] RIFA Solenopsis invicta Buren belongs to Hymenoptera Formicidae Leafcutter Ant Subfamily Myrmicinae Fire Ant Genus Solenopsis. As a recognized harmful invasive alien species, RIFA is a typical agricultural and forestry pest, causing serious damage to the invaded areas. harm. In my country, RIFA has invaded 11 provinces including Taiwan, Guangdong, Hong Kong, Macau, Fujian, Guangxi, Hunan, Hainan, Sichuan, Jiangxi and Yunnan. The red imported fire ants have strong destructive power and aggressiveness. They eat the seeds of plants, prey on invertebrates, release venom through their tail spines to bite humans and wild animals, and damage civil infrastructure by biting the insulation layer of electronic and electrical equipment. It poses ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N15/31C07K14/37A01N63/04A01P7/04C12R1/645
CPCA01N63/30C07K14/37C12N1/145C12R2001/645
Inventor 胡琼波谢伟文马竹炘周鹏飞
Owner SOUTH CHINA AGRI UNIV
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