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Combined diagnostic kit based on CCL8, CCL26 and CXCL6 genes and applied to glioblastoma and use method of combined diagnostic kit

A glioblastoma, CCL26 technology, applied in biochemical equipment and methods, microbial assay/test, etc., can solve the problem of unreported expression

Inactive Publication Date: 2017-12-08
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CCL8, CCL26 and CXCL6 are important members of the chemokine

Method used

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  • Combined diagnostic kit based on CCL8, CCL26 and CXCL6 genes and applied to glioblastoma and use method of combined diagnostic kit
  • Combined diagnostic kit based on CCL8, CCL26 and CXCL6 genes and applied to glioblastoma and use method of combined diagnostic kit
  • Combined diagnostic kit based on CCL8, CCL26 and CXCL6 genes and applied to glioblastoma and use method of combined diagnostic kit

Examples

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specific Embodiment

[0037] The experimental methods in the following examples are conventional methods unless otherwise specified, and the experimental reagents and materials involved are conventional biochemical reagents and materials unless otherwise specified.

[0038] 1. Differential expression of CCL8, CCL26 and CXCL6 in LGG and GBM compared with TCGA database

[0039] The Cancer Genome Atlas (TCGA) project team was originally composed of the National Cancer Institute (NCI) and the National Human Genome Research Institute (NHGRI), and developed into A very large data research platform. Low-grade glioma (LGG) and glioblastoma (GBM), as one of the earliest tumors studied, have a large number of comprehensive data results. The LGG and GBM data in TCGA come from (https: / / genome-cancer.ucsc.edu), in which LGG included 530 tumor samples, and GBM included 167 samples. The results of CCL8 gene transcription level are from the RNA-seq results of the Illumina HiSeq sequencing platform. figure 1 It ...

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Abstract

The invention particularly relates to a combined diagnostic kit based on CCL8, CCL26 and CXCL6 genes and applied to glioblastoma and a use method of the combined diagnostic kit. The kit comprises a PCR primer pair I for amplifying the CCL8, CCL26 and CXCL6 genes and a PCR primer pair II for amplifying the housekeeping gene GAPDH. The fact that the transcriptional expression levels of the CCL8, CCL26 and CXCL6 genes in LGG and GBM are obviously different is discovered for the first time, combined diagnosis is performed by detecting expression conditions of transcription levels of the CCL8, CCL26 and CXCL6 genes, and the malignant degree of a patient with glioblastoma can be judged.

Description

technical field [0001] The invention belongs to the technical field of detection kits, and in particular relates to a combined diagnosis kit for glioblastoma based on CCL8, CCL26 and CXCL6 genes and a use method thereof. Background technique [0002] Glioblastoma is the most malignant and most common primary brain tumor, accounting for about 50% of central nervous system tumors. Although a comprehensive program based on surgery combined with radiation and chemotherapy has become a treatment guideline, the prognosis of glioblastoma patients has not been significantly improved, and the average survival time is only 12 to 15 months. Patients with low-grade glioma (LGG) will develop into high-grade glioma (GBM) after a long or short period of time. At present, the commonly used methods to distinguish low-grade gliomas from high-grade gliomas mainly rely on histopathological diagnosis, but the results of pathological diagnosis are somewhat subjective and unreliable. Therefore, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/158
Inventor 张毅张超奇张震宋梦佳沈志博任飞飞何谦益赵启泰李红杨波樊锐太
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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