The application of ellagic acid and its synergy with triptolide in the preparation of anti-lung cancer drugs

A technology of tripterine and anti-lung cancer drugs, which is applied in the application field of ellagic acid and synergy with tripterine in the preparation of anti-lung cancer drugs, can solve the problems of less research on lung cancer, the inhibitory effect of ellagic acid on lung cancer, The mechanism of lung cancer activity and therapeutic effect is not clear, etc., to achieve the effect of tumor inhibition, proliferation inhibition, synergistic effect, and reduced dosage

Active Publication Date: 2019-10-11
CHINA AGRI UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0011] Therefore, chemoprevention is currently considered to be one of the most promising ways to prevent cancer. Specifically, ellagic acid has shown its chemopreventive activity in some cancers and investigations. Some studies have shown that ellagic acid has the effect on prostate cancer. , colon cancer and other tumors have inhibitory effect, but there are relatively few studies on lung cancer, and although ellagic acid has pharmacological activity in vitro, the mechanism of ellagic acid's inhibitory effect on lung cancer, anti-lung cancer activity and therapeutic effect has not yet been clarified , and there is no research and application on the combined effect of ellagic acid and tripterine in the treatment of lung cancer

Method used

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  • The application of ellagic acid and its synergy with triptolide in the preparation of anti-lung cancer drugs
  • The application of ellagic acid and its synergy with triptolide in the preparation of anti-lung cancer drugs
  • The application of ellagic acid and its synergy with triptolide in the preparation of anti-lung cancer drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Inhibitory effect of ellagic acid on proliferation of lung cancer cells

[0043] MTT assay (also known as MTT colorimetric method) was used to establish an in vitro cultured human lung cancer cell model with the primary cultured human lung cancer cell line (American Type Culture Collection, Manassas, VA, USA) as the experimental object. After trypsinization of lung cancer counts, adjust the cell density to 5×10 4 Cells / mL, plated on a 96-well plate, added 100 μL of cell suspension to each well, and replaced with fresh medium containing the corresponding ellagic acid concentration gradient (10, 25, 50 μM) after the cells were completely adhered to the wall, and placed at 37°C. Cultivate in a 5% carbon dioxide incubator for 48 hours, add 10 μL of MTT reagent about 3 hours before the test, measure the absorbance at 570 nm wavelength with a microplate reader and calculate the inhibition rate and half inhibition rate, from five lung cancer cells A549, H460, LLC, H1975, and H...

Embodiment 2

[0045] Acute Toxicity Experiment and Antitumor Effect of Ellagic Acid on Tumor-bearing Mice

[0046] In this experiment, 5-week-old BALB / c female nude mice were used. HOP62 cells were harvested, washed three times with PBS, resuspended in PBS, and adjusted to a density of 1×10 7 / mL, 100 μL of cells were inoculated subcutaneously on the right side of each nude mouse. When the tumor volume reaches a palpable size (about 100mm 3 ), mice were randomly divided into 3 groups: blank solvent control group (5% hydrogenated castor oil, 5% ethanol, 90% PBS), a total of 6 mice, ellagic acid administration group (40, 80mg / kg), 6 in each group Only, intragastric administration was carried out, administered once every other day, and administered continuously for 22 days. Changes in tumor length, width, and body weight were measured with a vernier caliper every other day. Tumor volume calculation formula: V = 1 / 2 (length × width 2 ). After the experiment, the nude mice were killed by ne...

Embodiment 3

[0049] Inhibitory effect of ellagic acid combined with tripterine on proliferation of lung cancer cells

[0050] Choose three low concentrations of ellagic acid: 10, 25 and 50 μM, and choose three low concentrations of tripterine: 0.5, 0.75 and 1 μM. Through the MTT experiment, different concentrations of ellagic acid and different concentrations of tripterine and the joint administration of different concentrations of ellagic acid and tripterine to inhibit ellagic acid and tripterine to treat lung cancer cells (HOP62 and H1975 lung cancer cell lines described in Example 1) respectively, placed in 37°C, 5% carbon dioxide incubator for 48h, measure the absorbance at 570nm wavelength with a microplate reader and calculate the inhibition rate and half inhibition rate, and detect the synergistic effect between ellagic acid and tripterine, as shown in the attached Figure 4 As shown, the experimental results show that: compared with ellagic acid alone and tripterine alone, the comb...

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Abstract

The invention discloses application of ellagic acid and tripterine for synergically preparing an anti-lung cancer drug. The ellagic acid exerts a better function and is used as a natural polyphenol material; in an animal experiment, the ellagic acid almost has no side effect and the serum index and the weight are both normal; under the synergic effect of ellagic acid and tripterine, the dosage of the tripterine can be reduced and no obvious side effect exists.

Description

technical field [0001] The invention relates to the field of natural polyphenolic substances, in particular to an application of ellagic acid and its synergy with tripterine in the preparation of anti-lung cancer drugs. Background technique [0002] Lung cancer is one of the main causes of cancer death in the world. More than one million patients die of lung cancer every year. The current systemic treatment is not effective and the prognosis is poor. The 5-year overall survival rate of lung cancer is only 15%, which is the highest in the world Cancer with the highest morbidity and mortality rate in the world has become the "first killer" that seriously threatens human health. In 2008 alone, there were 1.6 million new cases of lung cancer (accounting for 13% of all cancers that year), and the death cases It also reached 1.4 million (accounting for 18% of all cancers), and by 2010, the number of deaths from lung cancer rose to 1.5 million (accounting for 19% of all cancers in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/366A61K31/56A61P35/00
Inventor 黄卫东段婧战吉宬
Owner CHINA AGRI UNIV
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