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Pharmaceutical composition for synergistically inhibiting drug-induced liver injury induced by tetrandrine

A drug-induced liver injury, Tetrandrine technology, applied in the field of medicine, to achieve the effect of inhibiting liver injury, inhibiting apoptosis, and good application prospects

Pending Publication Date: 2022-03-04
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the specific inhibition of tetrandrine-induced liver injury by the combination of ursolic acid and glycyrrhetinic acid

Method used

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  • Pharmaceutical composition for synergistically inhibiting drug-induced liver injury induced by tetrandrine
  • Pharmaceutical composition for synergistically inhibiting drug-induced liver injury induced by tetrandrine
  • Pharmaceutical composition for synergistically inhibiting drug-induced liver injury induced by tetrandrine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The cells used in the present invention are HepG2 cells. In order to detect the effect of tetrandrine on the cell apoptosis, HepG2 cells were seeded in 96-well cell culture plates and divided into blank control group and tetrandrine administration group (8, 9, 10, 20, 30, 40, 50, 60, 70, 80 μM). When the cells grew to 80%-90%, different concentrations of tetrandrine were administered. After 24 hours of culture, the cell viability was detected using the CCK-8 cell viability detection kit, and the half inhibitory concentration IC was calculated using GraphPad Prism 5.0. 50 , the result is as figure 1 shown. Depend on figure 1 It can be seen that tetrandrine can inhibit the cell viability of HepG2 cells, and its IC 50 The value is 33.10 μM. Therefore, 30 μM tetrandrine was selected as the dosage for the subsequent cell injury model.

Embodiment 2

[0039] Study on the protective effect of ursolic acid and oleanolic acid on HepG2 cell injury induced by tetrandrine: HepG2 cells were seeded in 96-well plates and cultured overnight. The experiment was divided into blank group and tetrandrine group (30μM ) modeling group, different concentrations of ursolic acid (10 -10 、10 -9 、10 -8 、10 -7 、10 -6 、10 -5 、10 -4 mol / L) administration group, different concentrations of oleanolic acid (10 -10 、10 -9 、10 -8 、10 -7 、10 -6 、10 -5 、10 -4 mol / L) administration group, after 24 hours of administration and treatment, the cell viability was detected by CCK-8 kit, and the inhibitory effect of ursolic acid and oleanolic acid on tetrandrine-induced apoptosis was investigated. The result is as figure 2 As shown, compared with the blank group, tetrandrine can reduce the cell viability of HepG2 cells, at 10 -8 to 10 -5 In the range of mol / L, ursolic acid can significantly inhibit the apoptosis induced by tetrandrine, and the op...

Embodiment 3

[0041] Protective effect of glycyrrhetinic acid on Tetrandrine-induced HepG2 cell damage: HepG2 cells were seeded in 96-well plates and cultured overnight. The experiment was divided into blank group, Tetrandrine (30 μM) Ursolic acid (10 -10 、10 -9 、10 -8 、10 -7 、10 -6 、10 -5 、10 -4 mol / L) administration group, after administration and treatment for 24 hours, the cell viability was detected by CCK-8 kit, and the inhibitory effect of glycyrrhetinic acid on tetrandrine-induced apoptosis was investigated. The result is as image 3 As shown, compared with the blank group, tetrandrine can reduce the cell viability of HepG2 cells, at 10 -10 to 10 -6 Within the concentration range of mol / L, glycyrrhetinic acid can significantly inhibit the apoptosis induced by tetrandrine, and the optimal concentration is 10 -8 mol / L, the optimal concentration range is 10 -10 -10 -8 mol / L, indicating that low concentrations of glycyrrhetinic acid have a better inhibitory effect on tetrandr...

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Abstract

The invention discloses a pharmaceutical composition for synergistically inhibiting drug-induced liver injury induced by tetrandrine, and belongs to the technical field of medicines. The pharmaceutical composition comprises ursolic acid and glycyrrhetinic acid, or comprises oleanolic acid and glycyrrhetinic acid. The pharmaceutical composition can remarkably relieve liver HepG2 cell apoptosis induced by tetrandrine and mouse liver injury induced by tetrandrine, the two drugs have a synergistic effect, the protection effect is obviously superior to that of a single drug, an effective method is provided for guaranteeing the medication safety of a pharmaceutical preparation containing tetrandrine, and the pharmaceutical composition has a good application prospect. Good application prospects are realized in the field of hepatotoxicity prevention and treatment of medicines.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a pharmaceutical composition for synergistically inhibiting drug-induced liver injury induced by tetrandrine. Background technique [0002] Drug-induced liver injury, also known as drug-induced liver injury or drug-induced liver disease, refers to various prescription or over-the-counter chemical drugs, biological agents, traditional Chinese medicine, natural medicine, health products, dietary Liver injury induced by supplements, their metabolites, and even excipients has become an important link in the evaluation of drug safety due to the lack of effective treatment methods due to the unclear pathogenesis. Among them, the safety of drug-induced liver injury caused by Chinese herbal medicine continues to attract widespread attention. [0003] The pathogenesis of drug-induced liver injury is complex, usually summarized as direct hepatotoxicity and idiosyncratic hepat...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61P1/16
CPCA61K31/56A61P1/16A61K2300/00Y02A50/30
Inventor 白钢褚思梦姜民侯媛媛
Owner NANKAI UNIV
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