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A kind of Enterobacteriaceae and its application in water purification

A technology for purifying water and Enterobacteriaceae, applied in chemical instruments and methods, biochemical equipment and methods, bacteria, etc., can solve the problem of weak water purification ability in winter, and achieve effective and stable treatment, good microbial activity, and strong adaptability Effect

Active Publication Date: 2020-08-07
重庆华悦生态环境工程研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem of weak water purification ability in winter in the existing artificial wetland technology, the purpose of the present invention is to provide an Enterobacter and its application in the joint purification of water with artificial wetland at low temperature

Method used

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  • A kind of Enterobacteriaceae and its application in water purification
  • A kind of Enterobacteriaceae and its application in water purification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Screening and Identification of Example 1 Bacterial Strains

[0038] 1. Screening

[0039] 1) Add 0.3 g of yeast powder to 100 mL of the enterobacteria-containing mud sample, shake gently at 10°C at 100 rpm for enrichment and culture for 1 week, and use a microscope to detect the growth of the bacteria; the mud sample is taken from Lashan River, Jinan. Bacteria grow to measure the absorbance value of the bacterial culture solution at 600nm, if the obtained OD600 value is between 0.6-0.8.

[0040] 2) Filter the bacteria in the above culture solution by membrane filtration, and wash the bacteria with 20 mL of sterile water. Inoculate multiple 100mL enrichment media with 5% inoculum amount, culture at 10°C, and set no inoculation control (CK). The growth of bacteria was observed after two weeks of culture on a shaker at 100rpm.

[0041] 3) Gradually dilute the enriched culture medium by 1, 2, 3, 4, 5 (corresponding to 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 ), respec...

Embodiment 2

[0047] Purification of water body at low temperature in embodiment 2

[0048] 1. Lead the typical black and odorous water body (the black and odorous water body of Lashan River in Jinan) into the temperature-controlled artificial wetland (temperature is 10°C), and the surface of the wetland is filled with commercially available silicon-based porous filler with a thickness ≥ 10cm.

[0049] The wastewater quality is as follows: pH 7.0; chemical oxygen demand (COD), 55 mg / L; total nitrogen (TN), 20 mg / L, and total phosphorus (P), 2 mg / L.

[0050] 2. Inoculate the Enterobacteriaceae YWH-5 identified in Example 1 in the enrichment medium and cultivate it on a shaker at 100 rpm at 10°C until the bacterial concentration is 10 8 CFU / mL; then inoculate the bacterial solution (also known as the bacterial agent, the same as the bacterial agent described in the summary of the invention) into the silicon-based filler, the inoculum amount is 3L / m 3 . The hydraulic retention time is 3 hour...

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Abstract

The invention relates to an enterobacterium and its application in purifying water. The enterobacter number is YWH-5, and it is preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures. The preservation date is January 16, 2017, and the preservation number is CGMCC No.13631. Enterobacter YWH-5 of the present invention is used to grow on the surface of the silicon-based porous filler, and after being placed in the artificial wetland, it can efficiently absorb and degrade organic matter in black and odorous water at low temperature, purify the water quality, and the process flow does not need additional provision Electric energy, low operating costs, effective and stable processing.

Description

technical field [0001] The invention relates to microorganisms for purifying water bodies, in particular to an enterobacterium and its application in purifying water bodies. Background technique [0002] With the rapid development of economy and the acceleration of urbanization, the problems of water quality pollution and ecological degradation of rivers in many cities in my country are very prominent, and even seasonal and perennial black and odorous water bodies have appeared. The existing black and odorous water treatment methods mainly adopt plant purification and aeration treatment, the purification load is low, the treatment process requires additional power input, and the operating cost is high. In recent years, the constructed wetland technology, which can achieve better purification treatment without additional electric energy input, has been widely used in the treatment of black and odorous water bodies, and has achieved better treatment results. However, in some ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/32C12R1/01C02F101/30
CPCC02F3/32C02F3/34C02F2101/30C12N1/20C12N1/205C12R2001/01Y02W10/10
Inventor 叶文华刘兴宇嵩单于建华
Owner 重庆华悦生态环境工程研究院有限公司
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