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Luteolin-7-diglucuronide and application of composition of luteolin-7-diglucuronide and doxepin in preparation of drug for treating depression

A technology of glucuronide and luteolin is applied in the directions of drug combinations, medical preparations containing active ingredients, pharmaceutical formulations, etc. The effect of improving cell survival rate, improving curative effect and good application prospect

Inactive Publication Date: 2018-01-09
刘锐
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] According to existing reports, known traditional Chinese medicine monomer compounds icariin (Icariin, ICA), genistein (Genistein, Gen), puerarin (Puerarin, Pue), berberine (Berberine, Ber), resveratrol Alcohol (Resveratrol, Res), curcumin (Curcumin, Cur), luteolin (luteolin, Lut) all have certain antidepressant activity, but luteolin-7-diglucuronide has not been seen yet in antidepressant aspect. report

Method used

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  • Luteolin-7-diglucuronide and application of composition of luteolin-7-diglucuronide and doxepin in preparation of drug for treating depression
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  • Luteolin-7-diglucuronide and application of composition of luteolin-7-diglucuronide and doxepin in preparation of drug for treating depression

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Experimental program
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Effect test

Embodiment 1

[0017] 1 Experimental materials

[0018] Drug: Luteolin-7-diglucuronide, donated by Shanghai University of Traditional Chinese Medicine Resources and Quality Evaluation Laboratory of Chinese Medicine, with a purity of more than 98%; luteolin, purchased from Chengdu Desite Biotechnology Co., Ltd.; Ping, purchased from Shandong Renhetang Pharmaceutical Co., Ltd.

[0019] Cell line: SH-SY5Y human neuroblastoma cells, purchased from Wuhan Punuosai Life Science and Technology Co., Ltd.

[0020] 2 Experimental methods

[0021] SH-SY5Y cells in logarithmic growth phase and in good growth state were taken, washed with PBS, digested with 0.25% trypsin, and then rinsed with DMEM culture medium containing 15% fetal bovine serum by blowing and blowing the cells to make SH-SY5Y single cell suspension. Take 1×10 5 Inoculated in a 96-well culture plate at a concentration of 1 / ml, 100 μL per well, and placed the culture plate in CO 2 The culture was continued in the incubator, and after 2...

Embodiment 2

[0029] 1 Experimental materials

[0030] Drug: Luteolin-7-diglucuronide, donated by Shanghai University of Traditional Chinese Medicine Resources and Quality Evaluation Laboratory of Chinese Medicine, with a purity of more than 98%; luteolin, purchased from Chengdu Desite Biotechnology Co., Ltd.; Ping, purchased from Shandong Renhetang Pharmaceutical Co., Ltd.

[0031] Experimental animals: male SPF grade ICR mice, weighing 16-18 g, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., and were free to forage and drink, and were raised under natural light conditions at room temperature.

[0032] 2 Experimental methods

[0033] 2.1 Tail suspension experiment

[0034] The experimental mice were randomly divided into 6 groups, namely: low dose group of test drug, medium dose group of test drug, high dose group of test drug, luteolin group, doxepin group, test drug + doxepin group, negative control Group, 8 in each group. Luteolin-7-diglucuronide ...

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Abstract

The invention relates to a luteolin-7-diglucuronide and application of a composition of the luteolin-7-diglucuronide and doxepin in the preparation of a drug for treating depression. By means of a cell experiment, a mouse tail suspension experiment and a forced swimming experiment, the invention proves that the luteolin-7-diglucuronide has a protective effect on SH-SY5Y cells, can remarkably shorten the immobility time in mouse tail suspension and the immobility time in forced swimming, and has potential anti-depression activity, the effect is better than the effects of luteolin and the positive western medicine doxepin, moreover, because the luteolin-7-diglucuronide and the doxepin are administrated together, the effect is more obvious. The invention provides a novel drug for the treatment of clinical depression.

Description

technical field [0001] The invention relates to a medicine for treating depression, in particular to the application of luteolin-7-diglucuronide and its composition with doxepin in the preparation of medicine for treating depression. Background technique [0002] Luteolin-7-diglucuronide is a derivative of luteolin, its full name is luteolin-7-O-[β-glucuronosy (1→2)β-glucuronic acid], chemical Structural formula see figure 1 , the traditional Chinese medicine monomer compound exists in plants such as mignonette, honeysuckle, nepeta and the like. [0003] Depression, also known as depressive disorder, is the main type of mood disorder with significant and persistent low mood as the main clinical feature. It can be seen clinically that the mood is not commensurate with the situation. The depression can range from depressed to distraught, low self-esteem and depression, even pessimistic world-weariness, there may be suicide attempts or behaviors, and even stupor; some cases h...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61K31/335A61P25/24
Inventor 刘锐马燕戚先芳韩梅刘君
Owner 刘锐
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