Preparation method of multivalent egg yolk antibody for waterfowl

A yolk antibody, waterfowl technology, applied in antiviral agents, antiviral immunoglobulin, immunoglobulin from eggs, etc., can solve the problem that yolk antibody is difficult to prevent and treat reovirus at the same time, and achieve good clinical protection effect, The effect of reducing breeding costs and high antibody levels

Active Publication Date: 2018-01-16
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The present invention aims at the technical defects of the prior art, and provides a method for preparing a multi-linked yolk antibody for waterfowl, so as to solve the problem that the yolk antibody of the prior art is difficult to simultaneously prevent and treat reovirus, parvovirus, gosling plague virus, Technical issues for various diseases such as duck hepatitis virus

Method used

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  • Preparation method of multivalent egg yolk antibody for waterfowl
  • Preparation method of multivalent egg yolk antibody for waterfowl
  • Preparation method of multivalent egg yolk antibody for waterfowl

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Example 1: Preparation of multiple refined egg yolk antibodies

[0043] (1) Preparation of oil adjuvant inactivated vaccine

[0044] a. Preparation of inactivated reovirus suspension

[0045] Inoculate DEF cells prepared from 9-10-day-old SPF meat duck embryos with frozen-preserved new Muscovy duck-derived duck reovirus and new meat duck-derived duck reovirus after thawing and dilution, harvest diseased DEF cells after 36-72 hours , repeated freezing and thawing, centrifugation, 100KD hollow fiber ultrafiltration concentration, inactivation, and then stored in a refrigerator at low temperature for later use;

[0046] b. Preparation of inactivated parvovirus suspension

[0047] Inoculate DEF cells prepared from 9-10-day-old SPF Muscovy duck embryos with frozen-preserved Muscovy duck-derived parvovirus and gosling plague virus, after thawing and diluting, harvest diseased DEF cells for 36-72 hours, freeze-thaw repeatedly, centrifuge, Concentrate, inactivate, and then s...

Embodiment 2

[0063] Example 2: Therapeutic effect of multiple refined egg yolk antibodies

[0064] 2.1 Test method

[0065] 40 1-day-old muscovy ducks and 40 Cherry Valley meat ducks were randomly divided into 4 groups, the first group was the treatment group, the second group was the common antibody treatment group, the third group was the challenge group without treatment, and the fourth group was the no treatment group. Do not attack the poisonous group. The specific grouping conditions are shown in Table 1. The 1-day-old experimental animals were intramuscularly injected with multiple refined egg yolk antibodies, 0.5ml each, and 6-8 hours after inoculation, intramuscularly injected with the newly isolated new Muscovy duck-derived duck reovirus and the new Duck reovirus (including 10 2.0～2.5 EID 50 ), observed for 14 days, and recorded the incidence. The dead ducks were autopsied to observe the lesions of the liver and spleen, and the organ morphology was as follows Figure 1-8 sho...

Embodiment 3

[0075] A method for preparing a multi-linked yolk antibody for waterfowl, comprising the following steps:

[0076] 1) Prepare inactivated new Muscovy duck-derived duck reovirus, new meat duck-derived duck reovirus, Muscovy duck-derived parvovirus, goose plague virus, type Ⅰ duck hepatitis virus disease, and type Ⅲ duck hepatitis virus For venom, six equal volumes are mixed to obtain a mixed antigen solution;

[0077] 2) Take the white oil produced by the American Esso company, the model is MARCOL52, and boil it, and add 4 parts of the white oil quality Siban and 2 parts of the white oil quality hardener during the boiling process. Aluminum fatty acid to obtain a vaccine adjuvant;

[0078] 3) Mix the mixed antigen solution obtained in step 1) with the vaccine adjuvant obtained in step 2) in a ratio of 1:2 (v / v) to obtain a conjoint vaccine;

[0079] 4) Use the multi-vaccination vaccine obtained in step 3) to immunize laying hens once a month before laying eggs, and then immun...

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Abstract

The invention provides a preparation method of a multivalent egg yolk antibody for waterfowl. According to the technical scheme, the preparation method is characterized in that six virus antigens, covering various virus antigens such as avian reoviruses, parvovirus, gosling plague virus and duck hepatitis virus, are screened from some common and small-onset-day diseases of the waterfowl; on that basis, targeted design is carried out based on vaccine preparation, immunizing method and other perspectives, thus the valence level of each antibody in efficient immune eggs is guaranteed effectively,and the foundation is provided for clinical protection effect of the egg yolk antibody; meanwhile, the following purifying process is improved; a hollow fiber column with special molecular cut off isused for filtering and concentrating, so that most of macromolecular impurities can be removed while the antibody purity is increased, and as a result, the adverse reaction occurrence rate of the product can be decreased.

Description

technical field [0001] The invention relates to the technical field of poultry biological products, further relates to the preparation technology of egg yolk antibody, in particular to a method for preparing a multi-linked egg yolk antibody for waterfowl. Background technique [0002] Due to the rapid development of the human catering industry and the continuous improvement of people's consumption levels, the amount of waterfowl breeding is expanding day by day. However, the waterfowl breeding methods are relatively scattered and the breeding mode is relatively open, resulting in an increasing probability of many poultry diseases occurring in waterfowl. [0003] Reovirus is one of the main pathogens of chicken viral arthritis and tenosynovitis, belonging to Reoviridae, a kind of Reovirus genus. Since the strain was first isolated from the respiratory tract of chickens suffering from chronic respiratory diseases in 1954, a variety of avian reovirus isolates have been reported...

Claims

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Application Information

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IPC IPC(8): C07K16/02C07K16/10C07K16/08A61P31/14A61P31/20
Inventor 康亚男陈冰唐荣宏张立霞付旭彬郁宏伟梁武李守军
Owner TIANJIN RINGPU BIO TECH
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