Method for rapidly detecting lycoramine content of lycoris plant

Abstract

The invention relates to a method for rapidly detecting lycoramine content of a lycoris plant. The method comprises (1) preparation of a sample solution to be detected, (2) detection and (3) content calculation. The method can quickly extract and qualitatively and quantitatively analyze alkaloids such as lycoramine in the lycoris plant, greatly shorten the analysis time, can complete the detectionprocess within 6 minutes, has lycoramine retention time within 3.6 minutes, can get the detection result quickly, can greatly improve the speed and accuracy of analysis and can provide an effective and accurate analysis method for researching the accumulation rule and analysis and metabolism regulation and control of lycoramine in the lycoris plant.

Description

technical field [0001] The invention belongs to the field of detection of alkaloids, and in particular relates to a method for rapidly detecting the content of Lycosamine in plants of the genus Lycoris. Background technique [0002] Lycoris spp. is a bulbous herb of Amaryllidaceae (Amarylllidaceae), many of which are unique ornamental and medicinal flowers in China, rich in a variety of unique alkaloid components of Lycoris spp. So far, more than 500 alkaloid compounds with different structures have been isolated from Lycoris plants. Phytochemical studies have shown that these compounds have various pharmacological functions and have high medicinal value. Among them, ricolamin plays an important role in the treatment of Alzheimer's disease, post-poliomyelitis paralysis, and sciatic neuritis (Cortes et al., 2015). Up to now, wild Lycoris is still the most important source of medicinal Lycosylamine. Therefore, accurate qualitative and quantitative analysis of Lycosylamine con...

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Problems solved by technology

[0004] Li Mingkai (Establishment of Lycoris alkaloid analysis method and research on its biosynthesis, Anhui Agricultural University, 2012: 1-69) used thin-layer chromatography separation technology to realize the preliminary separation of alkaloids such as Lycorisamine, and Two-dimensional thin-layer chromatography was found to be more suitable than one-dimensional chromatography for the separation of many different alkaloids, mainly by migration with known alkaloid standards (galantamine, ricolamine, lycorine) To achieve preliminary qualitative analysis of the alkaloid species in the sample, but not for accurate structural and quantitative analysis

[0005] Yuan Juhong et al. (Yuan Juhong et al., Study on the difference of alkaloid content among different species of Amaryllis, Journal of Jiangxi Agricultural University, 32(3): 0560-0565, 2010) used high-performance liquid chromatography detection technology to pass samples and known biological The comparison of the retention time and peak area of ​​alkali standard substance under the same conditions carried out quantitative research on galantamine, ricolamine and lycorine in different plants of the genus Lycoris. The single extraction of Lycoris bulb material requires fresh 60g sample (water content about 90%), equivalent to 6g dry sample of Lycoris bulbs, needs to consume 75ml of ethyl acetate, 10ml of sodium hydroxide, 8ml of methanol, which consumes a lot of plant samples and reagents. At the same time, it only uses the method of HPLC , the content detection is carried out according to the retention time of the substance, and the substance to be tested cannot be accurately qualitative, so the quantitative result of the substance is unreliable, and the structure, qualitative and precise quantitative analysis cannot be carried out

[0006] Chun et al. (Chun et al., Isolation and identification of alkaloids and anthocyanins from flower and bulb of Lycoris radiata using HPLC and LC-ESI-MS, Journal of Agricultural Chemistry and Environment, 2 (1): 22-26, 2013) using HPLC - The ESI-MS method is used for quantitative analysis of lycosylamine in Lycoris. The elution time of lycosylamine is 46.9 minutes, and the HPLC analysis time is relatively long, which takes 60 minutes. According to the retention time and parent ion Compared with the method of Yuan Juhong et al., the accuracy of this method has been improved, but the obvious defect is that there may be many kinds of material components with the same elution time and precursor ion size, so there are still qualitative and quantitative accuracy. defect

[0007] Moreover, in the prior art, there are also the problems of long extraction time and cumbersome operations. For example, in the methods of Yuan Juhong, etc., the main extraction steps are drying, pulverization, sieving, wetting, extraction with ethyl acetate, reflux in a water bath, The steps of filtering out the extract, reflux in an ethyl acetate water bath, filtering out the extract, reflux in an ethyl acetate water bath, filtering out the extract, merging the reflux extract, rotating to dryness, cooling, washing with methanol to dissolve, and filtering, are The rapid quantitative analysis of licoramine is inconvenient

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