Purpose of hydrogen gas to preparation of renal fibrosis treatment preparation
A technology for renal fibrosis and fibrosis, which is applied in the field of preparation of preparations for treating renal fibrosis, and can solve the problems of unsatisfactory effect and the like
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[0045] Materials and methods: methanol (Sinopharm Chemical Reagent Co., Ltd.), paraformaldehyde (Sinopharm Chemical Reagent Co., Ltd.), ethanol (Sinopharm Chemical Reagent Co., Ltd.), cell culture medium: RPMI 1640 (Gibco), fetal bovine serum ( Hyclone), PBS phosphate buffered saline, trypsin (Shanghai Boguang Biotechnology Co., Ltd.), TGF-beta1 (R&D), DMSO (Sigma), PageRulerTM Prestmned Protein Ladder (Fermentas) 11.Mousemonoclonal to alpha muscle Actin (Abcam), E-cadhefin rabbit monoclonal antibody(Epitomics), PAl-1rabbit monoclonal antibody(Epitomics), HRP-labeled goat anti-rabbit IgG(H+L), HRP-labeled goat anti-mouse IgG(H+L)(Mingrui Biology), beta -Actin antibody (Sigma), BSA (Sigma), hydrogen peroxide (Sinopharm Chemical Reagent Co., Ltd.), hematoxylin stain (Wuhan Google Biotechnology Co., Ltd.), hydrochloric acid, ammonia (Sinopharm Chemical Reagent Co., Ltd.), neutral gum ( Google Bio), . Histochemical Kit DAB Chromogenic Reagent (DAKO);
[0046] Main instruments and...
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