38 results about "Cell studies" patented technology

A bioreactor system comprising a multi-well platform including an array of bioreactor units. The bioreactor system comprises a perfusion unit and a fluid source unit fluidly interconnected by a pumping unit. The perfusion unit comprises a multi-well plate including a plurality of main chambers configured to house or contain a cell culture and in each bioreactor unit an independent cell study or experiment may be performed.

The present invention pertains to an apparatus for imaging an object, comprising a probe via which an assay component may be delivered; a sensor to detect ion current; and means for controlling the position of the probe relative to the object in response to the ion current. Such apparatus can be used to image live cells, without affecting them, in solution, e.g., using light, wherein the distance between probe and cell is less than the wavelength of light.

The invention discloses a layered co-culture method for intramuscular fat cells and skeletal muscle cells of a mammal. The layered co-culture method comprises the steps: after washing and cutting a longissimus tissue on the back of a butchered animal into pieces, digesting the longissimus tissue by using type-I collagenase, and sieving a digested mixed solution; collecting filtrate, centrifuging, respectively collecting mature fat cell sap on the upper layer and muscle cells on the lower layer, respectively adding a proper amount of serum-free medium to dilute and wash, and centrifuging; adding the centrifugate on the upper layer and the muscle cell sap on the lower layer into a culture flask with the capacity of 25cm<2>, and culturing in a culture box with the temperature of 37 DEG C and the CO2 content of 5%. The mature fat cells can float and adhere to a ceiling surface on the upper layer because of small buoyancy, while muscle cells sink to the bottom of the culture flask to grow. The cell culture model can be used for layering and co-culturing the intramuscular fat cells and the muscle cells of the same muscle tissue of an animal in the same culture solution, so that a novel in-vitro cell research model is provided for researching the influence of mature fat cell secreta to muscle cell differentiation or the interaction between the muscle cells and the fat cells.

The invention relates to the technical field of biological cells, and discloses cell research centrifugal equipment for fixing a test tube through negative pressure. The cell research centrifugal equipment comprises a motor, the top of the motor is rotatably connected with a rotating shaft penetrating through a fixed table, the top of the rotating shaft is fixedly connected with a storage plate, storage cylinders arranged at equal intervals are inserted into the tops of the storage plate, storage grooves are formed in the tops of the storage cylinders, air cavities are formed in the lower portions of the interiors of the storage cylinders, threaded rods are rotatably connected to the inner top walls of the air cavities, pinions are fixedly connected to the bottoms of the threaded rods, andthe threaded rods is sleeved with pistons and ratchet wheels. When the pistons reach the bottom wall of the air cavity, the pistons stop moving downwards, the threaded rods drive the storage cylinders to rotate on the storage plate under the action of the pinions, so that the effect that the centrifugal test tube rotates while revolving around the center of the storage plate is achieved, the movement of the centrifugal test tube is more variable and complex, and the centrifugal effect of biological cells is better.

The invention relates to a preparation method and application of a nanowire biosensor, and belongs to the technical field of biological testing. According to the preparation method of the nanowire biosensor, the corresponding single-stranded nucleic acid probe is designed according to a target to be detected, and the target nucleic acid can be detected. Meanwhile, the continuous dynamic detectionof the target nucleic acid can be realized in situ through the nucleic acid melting and in-situ release technology of electric heating so that the concentration change condition of the target nucleicacid molecule is mastered. The nanowire biosensor prepared by the method can be used for conveniently and quickly preparing the required nanowire biosensor and is used for simply and quickly realizingunlinking and in-situ release of a biomarker and can be used for early diagnosis and prognosis of a detected person according to the concentration change of the specific biomarker aiming at the biomarker of a certain disease and can be widely applied to the fields of tumor early screening and prognosis monitoring, single cell research, embryo culture and development in assisted reproduction and the like.

The invention belongs to the field of biopharmacology, and relates to a purpose of hydrogen gas to preparation of renal fibrosis treatment medicine, in particular to a purpose of hydrogen gas to preparation of medicine for treating renal fibrosis caused by chronic nephrosis. A built animal model is subjected to biochemical index detection, isolated cell study, western blotting experiments, immunohistochemistry and data analysis after hydrogen gas intervening; the result shows that the hydrogen gas can realize the fibrosis inhibition on the pathological environment induction through an sirt1 path; the harm to the renal function unit in the fibrosis process is reduced through improving the sirt1; and the hydrogen gas can be further used for preparing a medicine preparation for treating renalfibrosis, particularly the renal fibrosis caused by chronic nephrosis.

The invention relates to a method for extracting anchorage-dependent cells. The method comprises the following steps of preprocessing polyacrylamide hydrogel, culturing anchorage-dependent cells, integrally excavating the cells and the adhered gel, removing surface gel and the like. According to the method, an anchorage-dependent cell extracting device suitable for the extracting method is also arranged. The extracting method disclosed by the invention is convenient and easy to operate, and the extracting of the anchorage-dependent cells can be completed without tedious steps, so that the possibility of mistaking is reduced; besides, the adopted extracting device is simple in structure and low in cost; and in addition, the extracting method is small in damage to the anchorage-dependent cells, and has important significance to cell research.

Small molecule ghrelin O-acyltransferase inhibitors found using an assay to detect ghrelin O-acyltransferase activity using an acrylodan-labeled peptide mimic of ghrelin that provides for high-throughput screening for ghrelin O-acyltransferase inhibitors and detection via high performance liquid chromatography. The newly discovered class of synthetic triterpenoids efficiently inhibits ghrelin acylation by GOAT and function as covalent reversible inhibitors of GOAT. In cell studies, the most potent members of this family of compounds efficiently block ghrelin acylation at submicromolar concentrations and offer a foundation for continued development and evaluation of novel hGOAT inhibitors as therapeutics targeting disorders such obesity, type II diabetes, gastroparesis, and Prader-Willi syndrome.

The invention relates to a related technology of medical molecular biology, and in particular relates to a cell research model constructed by adopting a siRNA jamming technology. A G6PD over-expressed ACHN stable cell strain disclosed by the invention is a G6PD over-expressed ACHN stable cell strain. The cell strain is obtained by pressurizing and screening a pBABE-puro-G6PD transfected human renal cell adenocarcinoma cell strain ACHN through puromycin, verifying Real-time PCR and Western blot, carrying out repeated passage and cryopreservation resuscitation. The G6PD expressed quantity of the obtained cell strain is increased by above 40 times. A construction method disclosed by the invention comprises the following steps of: 1, cloning a G6PD gene; 2, constructing a pBABE-puro-G6PD recombinant expressed vector; 3, carrying out transfection on an ACHN cell strain; and 4, screening and identifying an ACHN-G6PD cell strain. Construction of the stable cell strain provides a cell model for researching the relevance between G6PD and renal cell carcinoma and relevance mechanism and also can lay a foundation for generation and development mechanism of renal cell carcinoma, and discovery of a novel drug action target.

The invention discloses a test tube rack for biological cell research and application thereof, belonging to the technical field of biological cells. The test tube rack comprise a coil, wherein an outer mounting plate is movably connected to the interior of the coil, and a test tube ring is fixedly connected to the inner side of the outer mounting plate. According to the test tube rack for biological cell research and the application of the test tube rack, through the advantage of a self-stop function under the an overspeed condition, the centrifugal process of a test tube can be automaticallystopped through the structure of the test tube rack when the rotating speed of centrifugal equipment exceeds a rated value, so the problem that cell tissue samples are damaged due to overlarge centrifugal force is solved, cell tissue is prevented from being damaged, and cell tissue inactivation caused by stall is avoided. Compared with the prior art, the method has the advantages that the effectiveness of a centrifuged cell tissue sample is improved, the authenticity of experimental data is guaranteed, the credibility of an experimental result is improved, the problem of experimental process delay caused by cell tissue inactivation can be avoided, time and cell tissue resources are saved to a certain extent, and the requirements of biological cell research are better met.

The invention provides a preparation method of a novel cell additive and a novel cell additive product, and relates to the field of in vitro cell research for dyslipidemia relevant diseases. The method comprises the steps of adding a stearic acid component solution obtained by high-temperature dissolving to a BSA component solution obtained by room-temperature dissolving, performing shaking for uniform mixing, and after sufficient shaking combination, performing filtering to remove bacteria to obtain the novel cell additive product. A preparation method provided by the invention can solve thetechnical problems that in the prior art, in the preparation process of stearic acid or sodium stearate, solvent toxicity, difficult dissolving, easy separation and the like exist, and has the advantages that the concentration of the stearic acid or the sodium stearate is precise and controllable, products are good in stability, easy to store and the like, and the produced novel cell additive belongs to a ready-to-use product.

The invention relates to a daphnoretin micelle and a preparation method, content detection and application thereof. The daphnoretin nano-micelle is prepared by selecting a safe and degradable material PEG-PLA, and daphnoretin is prepared into a polymer micelle so as to achieve the purposes of increasing solubility and prolonging in-vivo action time. Meanwhile, the glycyrrhetinic acid coupled PEG-PLA is used as a carrier, and the nano preparation which is loaded with daphnoretin and has the liver cancer targeting property is prepared. Through in-vitro cell research, in-vivo targeting evaluation and preliminary pharmacodynamic research on the two drug-loaded micelles, the liver targeting drug delivery performance and the anti-tumor effect of the daphnoretin nano-micelle drug system are proved, and a theoretical basis is laid for in-vivo liver cancer targeting research of daphnoretin in the future; and a new thought is provided for the development of a new daphnoretin dosage form and a novel liver cancer targeting preparation.

Aiming at the technical defects of low target cell screening flux, large damage, easy loss and pollution of recovered cells and the like in the existing cell research technology, the invention provides a high-flux cell sorting and enriching device and a using method thereof. The device has the following advantages: 1) cell enrichment, recovery, capture and culture are integrated in a closed mode,so the problems of inter-link cell loss and pollution are solved; 2) on a cell separation module, by combining an inclined cell filter membrane and a fluid channel, the target cell flux is kept on thebasis of keeping the cell activity; and 3) a single cell (/cell cluster) capture and culture observation module solves the problem of cell tracking and positioning detection, and effectively culturesproliferative cells. The device is simple and convenient to operate, and provides equipment technical support for preparation and amplification of rare cell samples and single cell accurate researchin biomedicine and clinical diagnosis research.

The invention discloses a method for constructing an Alzheimer's disease (AD) cell model, and application of the Alzheimer's disease (AD) cell model. The method comprises the following steps of: constructing a recombinant vector containing a coding gene of APP protein, and integrating the coding gene of the APP protein into a genome of a host cell by using the recombinant vector, so as to construct and form the cell model. According to the invention, CHO (Chinese Hamster Ovary) and HFF (Human Foreskin Fibroblast) cell lines overexpressing APP genes are constructed through a gene technology to serve as the AD cell model; a lentivirus transfection mode is adopted, so that it can be guaranteed that the AD cell model has the durability and stability of AD pathological characteristics; moreover, the AD cell model disclosed by the invention can stably express and secrete A beta, can be used for a long time, has stable and continuous A beta deposition and tau protein phosphorylation characteristics, and can well simulate pathological characteristics of the Alzheimer's disease; and an in-vitro cell research platform possibility is provided for research, development and screening of Alzheimer's disease related drugs and research of mechanisms.

The invention relates to a monomolecular mechanics method for measuring the accurate length of human telomere. The method comprises the following steps of: firstly, extracting genome DNA of a target cell; digesting the genome by using combined enzyme digestion, and reserving telomere DNA; carrying out affinity labeling on the two ends of telomere DNA by adopting digoxin and biotin respectively soas to obtain a telomere DNA structure capable of being used for Monomolecular mechanical measurement; secondly, fixing the telomere structure between streptavidin-coated magnetic beads and the glass surface paved with the anti-digoxin antibody, and carrying out a DNA mechanical tensile experiment on a monomolecular instrument; and finally, using a worm model to perform length conversion between nanometer and basic group pairs on the actually measured telomere length, so as to realize accurate measurement of the telomere length. The invention belongs to the field of precise instrument measurement and analysis and cell molecular biology, and develops a new method for directly measuring the accurate length of telomere for cell research, health screening and clinical drug therapeutic effect evaluation.

The invention discloses a human orbit preadipocyte cell line, a construction method and application. The cell line is obtained by immortalizing human orbit preadipocytes. The construction method of the cell line comprises the following steps of step 1, separating and culturing human orbit preadipocytes from human orbit adipose tissues; step 2, transfecting human orbit preadipocytes with the vector connected with hTERT gene, so that the cells overexpress hTERT; and step 3, screening out human orbit preadipocytes with negative CD90 expression. The cell line can be used as a cell research model for drug screening.

A method for detecting an anti-human epidermal growth factor receptor 2 monoclonal antibody-MMAE conjugate neutralizing antibody in human serum includes steps of: 1) with SK-BR-3 cell line being a target cell, researching bioactivity of a medicine; 2) immunizing a machin with the medicine as an antigen and enhancing the immunization with a medicine F(ab)'2 fragment to prepare a positive polyclonalantibody; 3) pretreating a serum sample by using SPE extraction to eliminate serum interference, thus increasing stability of the method and drug tolerance capability; 4) developing a neutralizing antibody detection method on the basis of the medicine activity method, and applying the detection method to detection on the neutralizing antibody in the pretreated serum sample. The method is sourcedfrom cell level-based bioactivity method and can directly reflect the molecular mechanism of action (MoA) of the medicine. By using the SK-BR-3 cell that is more sensitive to medicine action, the method is improved in sensitivity. The positive control antibody is prepared by immunizing a non-human primate animal, so that comparability of the method with an actual sample is better. The serum sampleis detected after the pretreatment, so that the detection results are more stable and reliable.

The invention discloses an application of a Raw264.7 cell line capable of stably expressing different human ApoE genotypes in researching the influence of the ApoE genotypes on inflammation immunity, and a preparation method of the stably transfected cell line. The preparation method comprises the following steps: transfecting different human ApoE genotype plasmids ApoE2, ApoE3 and ApoE4 with EGFP labels and a control plasmid N1 into a Raw264.7 cell by using a transfection reagent; after a positive screening agent G418 is added for selective culture, a stably transfected cell strain is obtained through multiple times of sorting in a flow cytometry single cell sorting mode, and ApoE genotype and expression identification is carried out; the stably transfected cell is used for researching the influence of the ApoE genotype on NLRP3 inflammasome activation, endogenous melatonin synthesis, mitochondrial energy metabolism, pathogen removal and the like. The cell strain provides a good tool for further research on biological characteristics of different ApoE genotypes in the field of inflammation immunity and research and development of drugs.