24 results about "Cell studies" patented technology

The invention discloses an animal single cell data cell type annotation method, which is characterized by comprising the following steps: collecting Bulk RNA-seq data of purified cell types in various tissues and organs of a specific animal, and integrating the Bulk RNA-seq data into a reference data set after preprocessing; the method comprises the following steps: acquiring single-cell RNA-seq original data, screening the single-cell RNA-seq original data to obtain high-quality cells, screening high-variation genes from the high-quality cells, processing the high-variation genes, extracting principal components, performing dimensionality reduction on the principal components, and performing cell clustering based on a dimensionality reduction result to obtain cell clusters; and calculating expression similarity between the cell clusters and the reference data set based on the high-variation genes, determining initial cell types of the cell clusters according to a similarity result, and carrying out iterative tuning on the initial cell types with similar scores to obtain a final cell type annotation result. The method provides efficient and accurate technical support for animal single cell research.

The application discloses a 3D printing micro-nano temperature measuring robot and a preparation method and application thereof, and belongs to the technical field of micro-nano robots. The 3D printing micro-nano temperature measuring robot comprises a magnetic propeller and a temperature probe, and the temperature probe is distributed on the magnetic propeller. Compared with the traditional method, the application has the following significant technical advantages: 1) high efficiency: after the application is adopted, the resolution of the measurement of the internal temperature of cells is improved, and the temperature distribution can reach 0.1 µm in a single space monitoring process; 2) multifunctionality: the application can not only measure the internal temperature of cells, but also realize the distribution of the magnetic field in the cells, thereby providing more functional support for cell research and medical diagnosis.

The invention belongs to the technical field of biological medicines, and particularly relates to a human CDH3-targeted chimeric antigen receptor T cell and application thereof. Aiming at the problem that the curative effect of an existing human CDH3-targeted chimeric antigen receptor T cell on treating CDH3 positive tumors needs to be improved, the method comprises the following steps: firstly, obtaining two specific antibodies capable of specifically recognizing and combining cell surface CDH3 antigens by utilizing a hybridoma screening technology, and preparing CAR-T cells by utilizing the antibodies; researches show that the CAR-T cell has good killing activity on CDH3 positive tumors and can be applied to immunotherapy of the CDH3 positive tumors. The invention develops a new CDH3 antibody, provides a new choice for CDH3 positive tumor immunotherapy, and has important significance.

The utility model discloses a rotatable freezing tube rack for adipose-derived stem cells, which belongs to the technical field of stem cell research and comprises a base, a supporting rod is fixedly connected above the base, and a plurality of placing mechanisms are rotatably connected to the periphery of the supporting rod. After the placing mechanism is connected to the periphery of the supporting rod in a sleeving mode, the two L-shaped rods are moved oppositely, the L-shaped rods move to drive the limiting rods to move, the limiting rods move to extrude the reset springs, then the inserting rods are inserted into the inserting cavities, then the L-shaped rods are loosened, the reset springs lose the external force to drive the limiting rods to move and be inserted into the limiting grooves, and then the placing mechanism is placed. The inserting rod and the supporting rod can be inserted and fixed through the limiting rod, the fixed plug is limited and fixed to the end of the supporting rod under the connecting action of the ejector block, the fixed plug can be conveniently disassembled and assembled, and then workers can conveniently disassemble and assemble the placing mechanisms and the supporting rods of different numbers.

The invention relates to a plant tissue single cell nucleus preparation method, which comprises: (1) adding a cell nucleus extraction buffer solution to a plant tissue, and continuously crushing the plant tissue for 2-5 min to obtain a crushed sample; (2) collecting supernate from the sample, and collecting cell nucleuses from the supernate; (3) resuspending the cell nucleus by using a cell nucleus extraction buffer solution to prepare a cell nucleus suspension; (4) carrying out cell flow sorting on the cell nucleus suspension to obtain sorted cell nucleuses; wherein the cell nucleus extraction buffer solution comprises the following components with working concentrations: 1 * of NIB, 1 * of a protease inhibitor Cocktail, 0.4 U / [mu] L of an RNase inhibitor and 1 mM of DTT.

The invention provides a method for constructing a DF-1 cell line with miR-155 gene deletion based on a CRISPR / Cas9 technology, and belongs to the technical field of gene editing. The invention provides a method for knocking out a miR-155 gene in a DF-1 cell line by using a CRISPR-Cas9 system, which is characterized in that gRNA (guide ribonucleic acid) of a targeted miR-155 gene is designed and synthesized according to a miR-155 gene sequence, then a CRISPR-Cas9 recombinant plasmid containing the gRNA is constructed, and the CRISPR-Cas9 recombinant plasmid is transferred into a DF-1 cell to obtain the DF-1 with the miR-155 gene deleted. The homozygous miR-155 gene deletion cell strain obtained by the method provides an effective cell research model for further functional research of a disease-resistant candidate gene miR-155.

1. The name of the design product: cell force electric coupling analyzer (second generation machine). 2. The use of the design product: the design product is used for experimental instruments for high-throughput, multi-channel electrophysiological signal acquisition, and cell research in the field of biomedicine. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention discloses an AI-based human germ cell development and culture system evaluation method, which comprises the following steps: step 1, constructing a standardized reference map, step 2, establishing a germ cell development stage prediction model which comprises a transfer learning-based single cell annotation variation inference prediction module, a united prediction module combining single cell annotation variation inference with k nearest neighbor and a cell type classification module for feature selection optimization realize collaborative decision through a three-level confidence system; and step 3, in-vitro culture system evaluation, including construction of double indexes for quantitative evaluation and analysis of evaluation results. According to the method, a whole-process solution from single cell reference map construction, development stage prediction to culture system evaluation is realized, and the problems of reference data fragmentation, inaccurate development stage annotation and lack of quantitative standards of culture system data in the current germ cell research field are solved.

The application discloses a solution for separating fat cell nucleus and cytoplasm, and the solution comprises solution 1 and solution 2; wherein the solution 1 comprises 8-12 mM hydrochloride buffer, 0.03-0.15% Tween-20, 8-12 mM sodium chloride, 2-4 mM magnesium chloride, 0.8-2% bovine serum albumin and 0.4-0.6% ethyl phenyl polyethylene glycol; the solution 2 comprises 8-12 mM hydrochloride buffer, 8-25 mM sodium chloride, 1-15 mM magnesium chloride and 0.8-2.5% bovine serum albumin; the percentage is volume percentage. The application can realize the research on all cell types of fat tissues, greatly reduces the required amount of fat tissues and improves the possibility of carrying out single cell research. The application can be used for both transcriptome research and epigenetic research.

The invention relates to the technical field of microinjection, and discloses a self-adaptive dynamic adjustment automatic microinjection method, which comprises the step of transferring cells with mark point arrays formed on the surfaces in a culture dish containing marking substances. The temperature, humidity and vibration parameters of the environment where the cells are located are collected in real time through multiple sensors. The cells are irradiated with light sources of different wavelengths, and images are captured. Acquiring position information of cell surface mark points, and establishing and optimizing a cell three-dimensional dynamic model. And determining an injection target, planning a path and generating a control instruction. The flexible injection needle is controlled to move and inject substances, the cell state and environmental parameters are monitored in real time during injection, the upper computer adjusts related parameters according to a self-adaptive control algorithm, and the needle is withdrawn to the initial position after injection. The micro-injection precision and stability can be effectively improved, cell damage is reduced, the experiment success rate is increased, and powerful support is provided for cell research and biotechnology application.

The invention discloses poria cocos homogeneous polysaccharide as well as a preparation method and application thereof. The poria cocos homogeneous polysaccharide is separated and purified from poria cocos by combining an anion exchange column chromatography technology and a molecular sieve technology, and in-vitro cell research shows that the poria cocos homogeneous polysaccharide has a relatively strong immune activation effect on macrophages, can enhance the phagocytic effect of the macrophages, and can be used for preparing the immunopotentiator. And a basis is provided for research and development of pachymaran health functional products.

The invention relates to the technical field of plant biology, and particularly discloses a preparation and instantaneous conversion method of mulberry protoplast. The preparation method comprises the following steps: taking young leaves of mulberry tissue culture seedlings as materials, carrying out light-proof enzymolysis by adopting an optimized enzymatic hydrolysate combination, and then filtering, centrifuging and washing to obtain the high-activity protoplast. According to the instantaneous transformation method, exogenous plasmids are introduced into protoplasts by using a specific PEG / Ca < 2 + > transformation solution. According to the method, the interference of mulberry leaf polysaccharide polyphenol is effectively overcome by optimizing an enzymolysis system, and the yield and survival rate of the protoplast are remarkably improved; the instantaneous transformation efficiency is remarkably improved by improving a transformation system, and an efficient and stable single cell research platform is provided for gene function research and molecular breeding of mulberry trees.

The invention relates to a cell surface friction force measuring device manufactured by three-dimensional printing. The cell surface friction force measuring device comprises a runner layer and an electrode layer, the flow channel layer is provided with a focusing flow channel, a detection flow channel, a friction flow channel and a waste liquid flow channel; four detection electrodes are arranged on the electrode layer, each two detection electrodes form a pair, and the detection electrodes respectively comprise an excitation electrode and an induction electrode and are respectively vertical to the detection flow channel and the friction flow channel. The focusing flow channel is in an asymmetric sine flow channel shape, and cells can be focused at a specific position of the flow channel, so that the cells pass through the detection flow channel at the same position. The friction flow channel is directly connected with the waste liquid flow channel, and the detection flow channel is obliquely connected with the friction flow channel and the waste liquid flow channel. According to the cell surface friction force measuring device manufactured through 3D printing, the indicating friction force values of different types of cells can be quantitatively obtained, and the device is designed and manufactured based on the flow impedance technology and has extremely high detection flux. Therefore, the device has an extremely high application prospect in biochemical fields such as single cell research and the like.

This invention discloses an AI-based method for evaluating human germ cell development and culture systems, comprising: Step 1, constructing a standardized reference atlas; Step 2, establishing a germ cell development stage prediction model, including a single-cell annotation variational inference prediction module based on transfer learning, a joint prediction module combining single-cell annotation variational inference with k-nearest neighbors, and a cell type classification module optimized by feature selection. These three modules achieve collaborative decision-making through a three-level confidence system; Step 3, evaluating the in vitro culture system, including constructing dual indicators for quantitative evaluation and analyzing the evaluation results. This invention provides a complete solution from single-cell reference atlas construction and development stage prediction to culture system evaluation, solving three key technical bottlenecks in current germ cell research: fragmented reference data, inaccurate developmental stage annotation, and lack of quantitative standards for culture system data.

The invention discloses a mouse NK cell in-vitro culture method and application, and belongs to the technical field of cell culture. The method comprises the following two culture steps: firstly, culturing for 1.5-2.5 days by using an activated culture medium containing an MEMS alpha culture medium, 15-25v / v% of FBS, beta-ME and mIL-2 (optional mIL-15 and mIL-12); and culturing for 16.5-17.5 days by using an amplification culture medium containing an MEMS alpha culture medium, FBS, beta-ME and mIL-2, wherein the cell density is controlled to be less than or equal to 2E < 6 > / mL in the whole culture process. According to the culture method provided by the invention, the amplification efficiency of the mNK cells can be remarkably improved, the initial proliferation speed of a multi-factor activation group is higher, the cells are maintained in a normal form, the transduction efficiency is improved, and sufficient high-activity cells are provided for research of the mNK cells.

Cell-binding agents linked to beads are used to attach the beads to cells that are subsequently partitions. Methods, reaction mixtures, and kits as well as other aspects are provided.

This invention is an advanced bioimaging device allowing for the observation and analysis of living cells and intracellular reactions in real-time without the need for staining. It uses a combination of nanophotonic microscopy, nanobiological sensors, artificial intelligence processing, and ultra-sensitive spectroscopy technology to record and analyze intracellular changes without destroying the biological structure. The key feature of this technology is non-invasive and live imaging of cells and tissues without the need to add dyes or external chemicals that can affect the normal behavior of the cell. It offers higher resolution, no chemical interference, and the ability to automatically analyze data compared to traditional imaging methods such as fluorescence and phase contrast. It also includes cell research, drug development, personalized medicine, biotechnology, nanotechnology, and medical diagnostics. This invention is designed to study molecular, cellular, and biological processes in living cells and can simultaneously perform imaging and data analysis.

The invention discloses a method for observing local death of cyanobacteria cells by using a UV device, and relates to the technical field of cell research, and the method comprises the following steps: culturing cyanobacteria cells to a logarithmic phase to obtain a cyanobacteria sample; preparing an observation device; dropwise adding a cyanobacteria sample to the surface of the solidified culture medium, and air-drying and fixing; locally irradiating a sample area below the opening through a UV light source; sealing the sample through a cover glass and a second silicone rubber film sheet, and transferring the marking line to the cover glass; and observing the death process of cyanobacteria cells. Local precise UV stress of cyanobacteria protonema is realized by customizing a shading plate with a local opening, in-situ long-time stable observation is guaranteed by matching agar fixation and a sealed chamber design, and a continuous fluorescence microscopic imaging technology is combined; and the accurate visual tracking of the death time sequence, spatial distribution and death signal transmission process of different cells on the single protonema is realized.

The application discloses an influenza B HLA-B*1501 specific polypeptide and application thereof, and belongs to the field of immune detection. The application provides a specific polypeptide for influenza B, and the amino acid sequence of the specific polypeptide is ALIGASICF. A polypeptide-MHC tetramer is prepared by using the corresponding polypeptide. The polypeptide-MHC tetramer is used for detecting T cells of influenza B infection convalescents, and the positive rates are 9.94% and 8.03% respectively, which are obviously higher than 0.05% and 0.12% of the negative control group. The polypeptide-MHC tetramer effectively increases the affinity of the polypeptide-MHC and the specific T cell surface TCR, can be used as an effective tool for T cell evaluation, can be used for specific T cell separation and cloning, and can be combined with single cell sequencing technology to separate specific TCR and serve as a T cell activation reagent. The application has high application value in the T cell research of human groups after influenza B infection or vaccination.

The utility model relates to the technical field of biological experiments, and discloses a cell culture plate which comprises a heat preservation shell, a heat preservation cover is rotatably connected to the rear end of the top of the heat preservation shell, a plurality of separation grooves are formed in the front side of the heat preservation shell, and a plurality of support wheels are fixedly connected to the left side and the right side of each separation groove at equal intervals. A plurality of partition grooves are formed in the left side and the right side of the bottom of each support wheel, a plurality of square culture plates are arranged in the partition grooves, clamping communication assemblies are arranged on the rear sides of the square culture plates, sliding grooves are formed in the left side and the right side of the bottom of each square culture plate, and the sliding grooves are slidably connected to the tops of the support wheels. According to the cell culture device, the square culture plates can be flexibly taken, placed and combined through the separation grooves, the support wheels and the sliding grooves, co-culture of different cells is facilitated, the culture dish containing grooves are matched with various culture dishes, the flexibility, stability and operation convenience of cell culture experiments are integrally improved, and good conditions are provided for cell research.

This invention discloses a method for synthesizing 4',7-O-dimethylquercetin and its applications, belonging to the field of pharmaceutical synthesis and application. 4',7-O-dimethylquercetin is chemically synthesized using a partially substituted rutin intermediate as a raw material. Under 365 nm ultraviolet light irradiation, 4',7-O-dimethylquercetin can generate excessive amounts of reactive oxygen species (ROS). In vitro cell studies show that it inhibits cancer cell proliferation under ultraviolet light irradiation, indicating that this compound has good application prospects as a photosensitizer in cancer chemotherapy.

The invention relates to the technical field of biomedicine, in particular to a multiple myeloma isatuximab drug-resistant cell strain as well as a preparation method and application thereof. According to the present invention, the H929 cell is adopted as the research object, and the human multiple myeloma isatuximab drug-resistant cell strain H929 / IRH homo spoiens is constructed by using the repeated ADCC test; the cell model shows that the proliferative activity is obviously inhibited, but the clone formation efficiency and the cell migration ability are obviously improved. Due to the characteristic, the istuximab is an important tool for constructing a multiple myeloma drug resistance model in vitro, and an ideal experimental platform is provided for analyzing an istuximab drug resistance mechanism, screening candidate drugs for reversing drug resistance and developing a novel treatment strategy. The unique phenotype not only contributes to revealing the molecular basis of drug resistance, but also can provide a key cell research model for research and development of multiple myeloma drug resistance prevention and treatment products.

1. The name of the design product: cell in-situ culture and complex impedance detector. 2. The use of the design product: the design product is used for experimental instruments for high-throughput, multi-channel electrophysiological signal acquisition, and cell research in the field of biomedicine. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.