38 results about "Cell studies" patented technology

The invention discloses an animal single cell data cell type annotation method, which is characterized by comprising the following steps: collecting Bulk RNA-seq data of purified cell types in various tissues and organs of a specific animal, and integrating the Bulk RNA-seq data into a reference data set after preprocessing; the method comprises the following steps: acquiring single-cell RNA-seq original data, screening the single-cell RNA-seq original data to obtain high-quality cells, screening high-variation genes from the high-quality cells, processing the high-variation genes, extracting principal components, performing dimensionality reduction on the principal components, and performing cell clustering based on a dimensionality reduction result to obtain cell clusters; and calculating expression similarity between the cell clusters and the reference data set based on the high-variation genes, determining initial cell types of the cell clusters according to a similarity result, and carrying out iterative tuning on the initial cell types with similar scores to obtain a final cell type annotation result. The method provides efficient and accurate technical support for animal single cell research.

The invention provides a human bladder cancer vedicetumab drug-resistant cell strain as well as a construction method and application thereof. The cell strain is named as a human bladder cancer drug-resistant cell strain T24-RC48, and the preservation number of the cell strain is CCTCC (China Center For Type Culture Collection) NO: C2024180. During construction, a method of continuously inducing at low concentration and gradually increasing the drug concentration and the treatment time of the RC48 is adopted, so that the formation of the RC48 drug-resistant cells is effectively induced, and the construction method is simple to operate and can effectively make up for the defects of the existing method. The human bladder transitional cell carcinoma RC48 drug-resistant cell strain T24-RC48 constructed by the invention can be used for researching a bladder cancer drug-resistant mechanism, promoting the development of predictive biomarkers, evaluating the antitumor activity of drugs and preparing a tumor cell model or a tumor animal model; a cell research model is provided for screening, researching and developing effective drugs and methods for overcoming bladder cancer drug resistance, and good application prospects are achieved.

The invention relates to the field of cell analysis, in particular to a cell chamber real-time feedback analysis system based on multi-parameter fusion weighting. Comprising a multi-parameter acquisition module for acquiring cell growth related parameter information in a cell chamber; an artificial intelligence algorithm model is built in the weight calculation module, and the weights of the multiple pieces of cell growth related parameter information are dynamically adjusted through machine learning; the parameter fusion module is used for fusing the multiple pieces of cell growth related parameter information according to the weight calculated by the weight calculation module; the real-time feedback module is used for generating feedback information in real time according to the fusion information; the visual display module is used for carrying out visual display on the feedback information; and the adaptive learning module is used for collecting experimental result information in the cell culture process, so that the weight calculation module optimizes the artificial intelligence algorithm model. According to the method, related parameter information of growth of each cell is dynamically adjusted according to the actual condition of cell culture, and fusion is carried out according to the weight, so that the requirements of modern cell research and application are met.

The application discloses a 3D printing micro-nano temperature measuring robot and a preparation method and application thereof, and belongs to the technical field of micro-nano robots. The 3D printing micro-nano temperature measuring robot comprises a magnetic propeller and a temperature probe, and the temperature probe is distributed on the magnetic propeller. Compared with the traditional method, the application has the following significant technical advantages: 1) high efficiency: after the application is adopted, the resolution of the measurement of the internal temperature of cells is improved, and the temperature distribution can reach 0.1 µm in a single space monitoring process; 2) multifunctionality: the application can not only measure the internal temperature of cells, but also realize the distribution of the magnetic field in the cells, thereby providing more functional support for cell research and medical diagnosis.

The invention belongs to the technical field of biological medicines, and particularly relates to a human CDH3-targeted chimeric antigen receptor T cell and application thereof. Aiming at the problem that the curative effect of an existing human CDH3-targeted chimeric antigen receptor T cell on treating CDH3 positive tumors needs to be improved, the method comprises the following steps: firstly, obtaining two specific antibodies capable of specifically recognizing and combining cell surface CDH3 antigens by utilizing a hybridoma screening technology, and preparing CAR-T cells by utilizing the antibodies; researches show that the CAR-T cell has good killing activity on CDH3 positive tumors and can be applied to immunotherapy of the CDH3 positive tumors. The invention develops a new CDH3 antibody, provides a new choice for CDH3 positive tumor immunotherapy, and has important significance.

The utility model discloses a rotatable freezing tube rack for adipose-derived stem cells, which belongs to the technical field of stem cell research and comprises a base, a supporting rod is fixedly connected above the base, and a plurality of placing mechanisms are rotatably connected to the periphery of the supporting rod. After the placing mechanism is connected to the periphery of the supporting rod in a sleeving mode, the two L-shaped rods are moved oppositely, the L-shaped rods move to drive the limiting rods to move, the limiting rods move to extrude the reset springs, then the inserting rods are inserted into the inserting cavities, then the L-shaped rods are loosened, the reset springs lose the external force to drive the limiting rods to move and be inserted into the limiting grooves, and then the placing mechanism is placed. The inserting rod and the supporting rod can be inserted and fixed through the limiting rod, the fixed plug is limited and fixed to the end of the supporting rod under the connecting action of the ejector block, the fixed plug can be conveniently disassembled and assembled, and then workers can conveniently disassemble and assemble the placing mechanisms and the supporting rods of different numbers.

The invention relates to a plant tissue single cell nucleus preparation method, which comprises: (1) adding a cell nucleus extraction buffer solution to a plant tissue, and continuously crushing the plant tissue for 2-5 min to obtain a crushed sample; (2) collecting supernate from the sample, and collecting cell nucleuses from the supernate; (3) resuspending the cell nucleus by using a cell nucleus extraction buffer solution to prepare a cell nucleus suspension; (4) carrying out cell flow sorting on the cell nucleus suspension to obtain sorted cell nucleuses; wherein the cell nucleus extraction buffer solution comprises the following components with working concentrations: 1 * of NIB, 1 * of a protease inhibitor Cocktail, 0.4 U / [mu] L of an RNase inhibitor and 1 mM of DTT.

The invention provides a method for constructing a DF-1 cell line with miR-155 gene deletion based on a CRISPR / Cas9 technology, and belongs to the technical field of gene editing. The invention provides a method for knocking out a miR-155 gene in a DF-1 cell line by using a CRISPR-Cas9 system, which is characterized in that gRNA (guide ribonucleic acid) of a targeted miR-155 gene is designed and synthesized according to a miR-155 gene sequence, then a CRISPR-Cas9 recombinant plasmid containing the gRNA is constructed, and the CRISPR-Cas9 recombinant plasmid is transferred into a DF-1 cell to obtain the DF-1 with the miR-155 gene deleted. The homozygous miR-155 gene deletion cell strain obtained by the method provides an effective cell research model for further functional research of a disease-resistant candidate gene miR-155.

1. The name of the design product: cell force electric coupling analyzer (second generation machine). 2. The use of the design product: the design product is used for experimental instruments for high-throughput, multi-channel electrophysiological signal acquisition, and cell research in the field of biomedicine. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention relates to a high-porosity and high-connectivity porous medium material for intracellular delivery of therapeutic cells and a preparation method of the high-porosity and high-connectivity porous medium material. According to the microchannel, a porous polymer medium is prepared in a hard pipeline through a hard template method, and the specific preparation method comprises the following steps: stirring and mixing a thermosetting polymer and alcohol-soluble particles, performing high-temperature curing, removing the alcohol-soluble particles through absolute ethyl alcohol, forming a porous structure, and constructing the microchannel for intracellular delivery of animal cells. The channel with the porous medium material has excellent conductivity, and the connectivity between pore diameters can reach 98%. And the pore size distribution is wide and is between 5 microns and 50 microns. On the premise of effectively enhancing the permeability of cell membranes, good cell activity is ensured, efficient and safe intracellular delivery is realized, the highest delivery efficiency can reach 91.2%, the highest cell survival rate can reach 97.2%, and the net yield can reach 88.6%. Meanwhile, the method is simple in preparation process, convenient to operate and particularly suitable for the fields of gene therapy, drug development, cell research and the like.

The invention discloses an AI-based human germ cell development and culture system evaluation method, which comprises the following steps: step 1, constructing a standardized reference map, step 2, establishing a germ cell development stage prediction model which comprises a transfer learning-based single cell annotation variation inference prediction module, a united prediction module combining single cell annotation variation inference with k nearest neighbor and a cell type classification module for feature selection optimization realize collaborative decision through a three-level confidence system; and step 3, in-vitro culture system evaluation, including construction of double indexes for quantitative evaluation and analysis of evaluation results. According to the method, a whole-process solution from single cell reference map construction, development stage prediction to culture system evaluation is realized, and the problems of reference data fragmentation, inaccurate development stage annotation and lack of quantitative standards of culture system data in the current germ cell research field are solved.

The application discloses a solution for separating fat cell nucleus and cytoplasm, and the solution comprises solution 1 and solution 2; wherein the solution 1 comprises 8-12 mM hydrochloride buffer, 0.03-0.15% Tween-20, 8-12 mM sodium chloride, 2-4 mM magnesium chloride, 0.8-2% bovine serum albumin and 0.4-0.6% ethyl phenyl polyethylene glycol; the solution 2 comprises 8-12 mM hydrochloride buffer, 8-25 mM sodium chloride, 1-15 mM magnesium chloride and 0.8-2.5% bovine serum albumin; the percentage is volume percentage. The application can realize the research on all cell types of fat tissues, greatly reduces the required amount of fat tissues and improves the possibility of carrying out single cell research. The application can be used for both transcriptome research and epigenetic research.

The invention relates to the technical field of microinjection, and discloses a self-adaptive dynamic adjustment automatic microinjection method, which comprises the step of transferring cells with mark point arrays formed on the surfaces in a culture dish containing marking substances. The temperature, humidity and vibration parameters of the environment where the cells are located are collected in real time through multiple sensors. The cells are irradiated with light sources of different wavelengths, and images are captured. Acquiring position information of cell surface mark points, and establishing and optimizing a cell three-dimensional dynamic model. And determining an injection target, planning a path and generating a control instruction. The flexible injection needle is controlled to move and inject substances, the cell state and environmental parameters are monitored in real time during injection, the upper computer adjusts related parameters according to a self-adaptive control algorithm, and the needle is withdrawn to the initial position after injection. The micro-injection precision and stability can be effectively improved, cell damage is reduced, the experiment success rate is increased, and powerful support is provided for cell research and biotechnology application.

The present invention relates to a method of photoinduced micro-sculpting of a hydrogel, said method comprising the steps of: i) provision of a hydrogel having a plurality of reactive groups each selected from the group consisting of a -CH group, a -NH group, an -OH group and a combination of two or more thereof, said hydrogel having a surface to be micro-sculpted; ii) provision of a photo-activator; iii) contacting the surface of the hydrogel of step i) with the photo-activator of step ii); and iv) illumination of said photo-activator on said surface using a digital mask and UV light to photo-excite the photo-activator to induce micro-sculpting of said hydrogel. Moreover, the invention relates to a micro-sculpted hydrogel obtainable or directly obtained by the method according to the invention. In addition, the invention relates to the use of a micro-sculpted hydrogel as a construct for cell studies, materials and biomaterials applications, tissue engineering, and soft robotics.

The present invention discloses an application of MK2206 in regulating the transition of cell fate in early embryonic development, and belongs to the field of biotechnology. The present invention reprograms pluripotent stem cells into a totipotent cell by adding MK2206. The present invention regulates the metabolic pathway method through the action of the MK2206 small molecule, and can effectively regulate the transition of cell fate from pluripotency to totipotency without gene editing. Specifically: MK2206 inhibits the AKT signaling pathway, thereby increasing the expression of the 2C gene. At the same time, MK2206 also inhibits glycolysis, promotes the accumulation of TCA cycle intermediates, and promotes the transition from pluripotency to totipotency. The present invention provides a new method to accurately regulate cell fate transition and metabolic regulation, and provides a new technical means for regenerative medicine, stem cell research and optimization of embryonic development.

The invention discloses poria cocos homogeneous polysaccharide as well as a preparation method and application thereof. The poria cocos homogeneous polysaccharide is separated and purified from poria cocos by combining an anion exchange column chromatography technology and a molecular sieve technology, and in-vitro cell research shows that the poria cocos homogeneous polysaccharide has a relatively strong immune activation effect on macrophages, can enhance the phagocytic effect of the macrophages, and can be used for preparing the immunopotentiator. And a basis is provided for research and development of pachymaran health functional products.

The invention relates to the technical field of plant biology, and particularly discloses a preparation and instantaneous conversion method of mulberry protoplast. The preparation method comprises the following steps: taking young leaves of mulberry tissue culture seedlings as materials, carrying out light-proof enzymolysis by adopting an optimized enzymatic hydrolysate combination, and then filtering, centrifuging and washing to obtain the high-activity protoplast. According to the instantaneous transformation method, exogenous plasmids are introduced into protoplasts by using a specific PEG / Ca < 2 + > transformation solution. According to the method, the interference of mulberry leaf polysaccharide polyphenol is effectively overcome by optimizing an enzymolysis system, and the yield and survival rate of the protoplast are remarkably improved; the instantaneous transformation efficiency is remarkably improved by improving a transformation system, and an efficient and stable single cell research platform is provided for gene function research and molecular breeding of mulberry trees.

The invention relates to a cell surface friction force measuring device manufactured by three-dimensional printing. The cell surface friction force measuring device comprises a runner layer and an electrode layer, the flow channel layer is provided with a focusing flow channel, a detection flow channel, a friction flow channel and a waste liquid flow channel; four detection electrodes are arranged on the electrode layer, each two detection electrodes form a pair, and the detection electrodes respectively comprise an excitation electrode and an induction electrode and are respectively vertical to the detection flow channel and the friction flow channel. The focusing flow channel is in an asymmetric sine flow channel shape, and cells can be focused at a specific position of the flow channel, so that the cells pass through the detection flow channel at the same position. The friction flow channel is directly connected with the waste liquid flow channel, and the detection flow channel is obliquely connected with the friction flow channel and the waste liquid flow channel. According to the cell surface friction force measuring device manufactured through 3D printing, the indicating friction force values of different types of cells can be quantitatively obtained, and the device is designed and manufactured based on the flow impedance technology and has extremely high detection flux. Therefore, the device has an extremely high application prospect in biochemical fields such as single cell research and the like.

This invention discloses an AI-based method for evaluating human germ cell development and culture systems, comprising: Step 1, constructing a standardized reference atlas; Step 2, establishing a germ cell development stage prediction model, including a single-cell annotation variational inference prediction module based on transfer learning, a joint prediction module combining single-cell annotation variational inference with k-nearest neighbors, and a cell type classification module optimized by feature selection. These three modules achieve collaborative decision-making through a three-level confidence system; Step 3, evaluating the in vitro culture system, including constructing dual indicators for quantitative evaluation and analyzing the evaluation results. This invention provides a complete solution from single-cell reference atlas construction and development stage prediction to culture system evaluation, solving three key technical bottlenecks in current germ cell research: fragmented reference data, inaccurate developmental stage annotation, and lack of quantitative standards for culture system data.

The invention discloses a mouse NK cell in-vitro culture method and application, and belongs to the technical field of cell culture. The method comprises the following two culture steps: firstly, culturing for 1.5-2.5 days by using an activated culture medium containing an MEMS alpha culture medium, 15-25v / v% of FBS, beta-ME and mIL-2 (optional mIL-15 and mIL-12); and culturing for 16.5-17.5 days by using an amplification culture medium containing an MEMS alpha culture medium, FBS, beta-ME and mIL-2, wherein the cell density is controlled to be less than or equal to 2E < 6 > / mL in the whole culture process. According to the culture method provided by the invention, the amplification efficiency of the mNK cells can be remarkably improved, the initial proliferation speed of a multi-factor activation group is higher, the cells are maintained in a normal form, the transduction efficiency is improved, and sufficient high-activity cells are provided for research of the mNK cells.

The disclosure relates to high-strength polyolefin composite fibers, which fibers have a fiber body comprising a composition consisting of polyolefin; 1-30 mass % of bioceramic particles having particle size D50 of 0.01-10 μm; at most 0.05 mass % of residual spin solvent; optionally 0-3 mass % of other additives; and wherein the sum of a)-d) is 100 mass %; and which fibers have bioceramic particles exposed at their surface, and show bioactivity. The composite fibers based on a composition of polyolefin with bioceramic particles mixed therein show particles being exposed at the fiber surface by techniques like AFM and XPS, and although apparently only a relatively small amount of bioceramic particles is exposed at the fiber surface, this appears sufficient for effective interaction with their environment and stimulating a positive biological response as demonstrated by in vitro cell studies.The present disclosure also concerns a method of making the high-strength composite fibers via a gel spinning process, fibrous articles comprising said bioactive composite fibers. Further embodiments concern use of these fibrous articles as a component of a medical implant or as a medical implant, especially as permanent high-strength orthopedic implants for repairing bone fractures or torn ligaments or tendons. Other embodiments include medical devices or implants comprising said fibrous articles.

Disclosed are compositions and methods for studying a Tat gene. Specifically, the disclosure provides a vector comprising a double-stranded nucleic acid construct which comprises a Tat gene and a green fluorescent protein (GFP) reporter element, and further wherein the double-stranded nucleic acid construct comprising AAVS1 (adeno-associated virus integration site, a safe harbor locus) arms that flank on both sides of the Tat gene and the reporter element for integration at the human AAVS1 site by homologus recombination. Further provided are methods of using a cell comprising the vector for studying the effects of exogenous conditions on expression of the Tat gene.

The utility model relates to the technical field of stem cell research, and discloses a cell spinner bottle rack for stem cell technical research, which comprises a transfer box, a fixed base is arranged in the transfer box, first connecting pieces are fixedly connected to the periphery of the upper end of the fixed base, and folding frames are arranged on two sides of the upper end of the fixed base. The two ends of the two folding frames are rotationally connected with the interiors of the four first connecting pieces correspondingly, the other two ends of the two folding frames are rotationally connected with second connecting pieces correspondingly, a placing plate is slidably connected to the upper portion of the interior of the transfer box, and the periphery of the lower end of the placing plate is fixedly connected with the upper ends of the four second connecting pieces correspondingly. When the sample bottle transfer device is used for transferring sample bottles, the placing plate can be adjusted to adapt to different types of sample bottles, so that the practicability of the device is greatly improved, and the transfer cost is reduced.

The present invention relates to the technical field of storage equipment attachments, and in particular to an ultra-low temperature cell transport and storage device for stem cell research, which can drive an ice box to drive a connecting rod to rotate by pushing a box body, and drive a heat preservation device to slide in a working chamber to adjust the heat preservation position by pressing the connecting rod and a pressure plate, so as to improve the efficiency of ice preservation, thereby improving the working efficiency of the equipment and increasing the practicality. The invention comprises a box body and a heat preservation device, a working chamber is arranged in the box body, and the heat preservation device is evenly installed in the working chamber. The heat preservation device also includes an ice box, a connecting rod, a box door, a top plate, a connecting seat, a pushing block, a pushing device, a sliding block and a heat preservation furnace, connecting holes are arranged at both ends of the working chamber, a plurality of connecting rods are respectively rotatably connected to the corresponding connecting holes, a plurality of connecting rods are evenly installed on the corresponding ice boxes and are rotatably connected to the corresponding ice boxes, the right end of the box door is hinged to the right end of the box body, and a hinge seat is arranged on the box door.

The invention discloses a preparation method suitable for dissociation and purification of madrepore cells. The preparation method comprises the following steps: dissecting living madrepore; carrying out digestion treatment on the sample by using an enzyme mixed solution; centrifuging and resuspending to obtain a cell suspension; effective separation of zooxanthellae and coral cells is realized through density gradient centrifugation by using 6% and 15% hydroxyethyl starch solutions; and washing, centrifuging and resuspending the separated coral cells to finally obtain a coral single-cell suspension. The invention provides a novel method for separating single cells of a madrepore-zooxanthellae symbiotic system based on an enzyme mixed solution and hydroxyethyl starch density gradient centrifugation method. Compared with single enzyme digestion, the enzyme mixed solution has better performance in the aspects of maintaining cell morphology and separating efficiency. The density gradient centrifugation of the optimized hydroxyethyl starch further improves the separation purity and efficiency of pure coral cells and zooxanthellae symbiotic coral cells, so that the high-quality single-cell suspension can be rapidly prepared, and reliable and efficient technical support is provided for the research of madrepore single cells.

The invention provides a radiation-resistant mouse colon cancer cell strain as well as a preparation method and application thereof, the cell strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 64962. On the basis of the parent MC38 cell strain, the radiation-resistant tumor cell strain is constructed by a method of forming subcutaneous tumor in vivo for many times in an animal model, and the radiation-resistant cell strain MC38-R screened by the invention has relatively high radiotherapy resistance, can better simulate the resistance of the tumor of an actual patient to radiotherapy, and can be used for preparing the radiation-resistant tumor cell strain. And the reliability of the radiotherapy resistance cell research model is improved.

The utility model relates to the technical field of stem cell sampling, and discloses a sampling device based on human body stem cell research, which comprises a needle cylinder and a needle head fixedly mounted in the middle of one side of the needle cylinder, a piston is slidably mounted in an inner cavity of the needle cylinder, and a push-pull rod is fixedly mounted in the middle of one side of the piston. Through mutual cooperation of a connecting rod, a rectangular frame, a movable plate, a clamping ball, a fixed plate, a T-shaped pull rod and a reset spring, the circular plate can be clamped and fixed after moving to a certain position, discomfort of a patient caused by excessive force of a worker is prevented, the use effect of the device is improved, and the device is worthy of popularization and application. Through the arrangement of the sampling tube, continuous extraction and collection of stem cells can be realized, the sampling efficiency is improved, through the mutual cooperation of the fixing ring, the threaded rod, the knob, the threaded sleeve, the guide plate, the T-shaped guide rod, the circular ring, the sliding plate and the positioning rod, the sampling tube can be quickly mounted and dismounted, a new sampling tube can be conveniently replaced, and the sampling efficiency is improved. The operation is convenient.

Cell-binding agents linked to beads are used to attach the beads to cells that are subsequently partitions. Methods, reaction mixtures, and kits as well as other aspects are provided.

This invention is an advanced bioimaging device allowing for the observation and analysis of living cells and intracellular reactions in real-time without the need for staining. It uses a combination of nanophotonic microscopy, nanobiological sensors, artificial intelligence processing, and ultra-sensitive spectroscopy technology to record and analyze intracellular changes without destroying the biological structure. The key feature of this technology is non-invasive and live imaging of cells and tissues without the need to add dyes or external chemicals that can affect the normal behavior of the cell. It offers higher resolution, no chemical interference, and the ability to automatically analyze data compared to traditional imaging methods such as fluorescence and phase contrast. It also includes cell research, drug development, personalized medicine, biotechnology, nanotechnology, and medical diagnostics. This invention is designed to study molecular, cellular, and biological processes in living cells and can simultaneously perform imaging and data analysis.

The invention discloses a method for observing local death of cyanobacteria cells by using a UV device, and relates to the technical field of cell research, and the method comprises the following steps: culturing cyanobacteria cells to a logarithmic phase to obtain a cyanobacteria sample; preparing an observation device; dropwise adding a cyanobacteria sample to the surface of the solidified culture medium, and air-drying and fixing; locally irradiating a sample area below the opening through a UV light source; sealing the sample through a cover glass and a second silicone rubber film sheet, and transferring the marking line to the cover glass; and observing the death process of cyanobacteria cells. Local precise UV stress of cyanobacteria protonema is realized by customizing a shading plate with a local opening, in-situ long-time stable observation is guaranteed by matching agar fixation and a sealed chamber design, and a continuous fluorescence microscopic imaging technology is combined; and the accurate visual tracking of the death time sequence, spatial distribution and death signal transmission process of different cells on the single protonema is realized.