13 results about "Cell studies" patented technology

The utility model discloses a rotatable freezing tube rack for adipose-derived stem cells, which belongs to the technical field of stem cell research and comprises a base, a supporting rod is fixedly connected above the base, and a plurality of placing mechanisms are rotatably connected to the periphery of the supporting rod. After the placing mechanism is connected to the periphery of the supporting rod in a sleeving mode, the two L-shaped rods are moved oppositely, the L-shaped rods move to drive the limiting rods to move, the limiting rods move to extrude the reset springs, then the inserting rods are inserted into the inserting cavities, then the L-shaped rods are loosened, the reset springs lose the external force to drive the limiting rods to move and be inserted into the limiting grooves, and then the placing mechanism is placed. The inserting rod and the supporting rod can be inserted and fixed through the limiting rod, the fixed plug is limited and fixed to the end of the supporting rod under the connecting action of the ejector block, the fixed plug can be conveniently disassembled and assembled, and then workers can conveniently disassemble and assemble the placing mechanisms and the supporting rods of different numbers.

The invention relates to a plant tissue single cell nucleus preparation method, which comprises: (1) adding a cell nucleus extraction buffer solution to a plant tissue, and continuously crushing the plant tissue for 2-5 min to obtain a crushed sample; (2) collecting supernate from the sample, and collecting cell nucleuses from the supernate; (3) resuspending the cell nucleus by using a cell nucleus extraction buffer solution to prepare a cell nucleus suspension; (4) carrying out cell flow sorting on the cell nucleus suspension to obtain sorted cell nucleuses; wherein the cell nucleus extraction buffer solution comprises the following components with working concentrations: 1 * of NIB, 1 * of a protease inhibitor Cocktail, 0.4 U / [mu] L of an RNase inhibitor and 1 mM of DTT.

1. The name of the design product: cell force electric coupling analyzer (second generation machine). 2. The use of the design product: the design product is used for experimental instruments for high-throughput, multi-channel electrophysiological signal acquisition, and cell research in the field of biomedicine. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The application discloses a solution for separating fat cell nucleus and cytoplasm, and the solution comprises solution 1 and solution 2; wherein the solution 1 comprises 8-12 mM hydrochloride buffer, 0.03-0.15% Tween-20, 8-12 mM sodium chloride, 2-4 mM magnesium chloride, 0.8-2% bovine serum albumin and 0.4-0.6% ethyl phenyl polyethylene glycol; the solution 2 comprises 8-12 mM hydrochloride buffer, 8-25 mM sodium chloride, 1-15 mM magnesium chloride and 0.8-2.5% bovine serum albumin; the percentage is volume percentage. The application can realize the research on all cell types of fat tissues, greatly reduces the required amount of fat tissues and improves the possibility of carrying out single cell research. The application can be used for both transcriptome research and epigenetic research.

The invention discloses poria cocos homogeneous polysaccharide as well as a preparation method and application thereof. The poria cocos homogeneous polysaccharide is separated and purified from poria cocos by combining an anion exchange column chromatography technology and a molecular sieve technology, and in-vitro cell research shows that the poria cocos homogeneous polysaccharide has a relatively strong immune activation effect on macrophages, can enhance the phagocytic effect of the macrophages, and can be used for preparing the immunopotentiator. And a basis is provided for research and development of pachymaran health functional products.

The invention relates to the technical field of plant biology, and particularly discloses a preparation and instantaneous conversion method of mulberry protoplast. The preparation method comprises the following steps: taking young leaves of mulberry tissue culture seedlings as materials, carrying out light-proof enzymolysis by adopting an optimized enzymatic hydrolysate combination, and then filtering, centrifuging and washing to obtain the high-activity protoplast. According to the instantaneous transformation method, exogenous plasmids are introduced into protoplasts by using a specific PEG / Ca < 2 + > transformation solution. According to the method, the interference of mulberry leaf polysaccharide polyphenol is effectively overcome by optimizing an enzymolysis system, and the yield and survival rate of the protoplast are remarkably improved; the instantaneous transformation efficiency is remarkably improved by improving a transformation system, and an efficient and stable single cell research platform is provided for gene function research and molecular breeding of mulberry trees.

The invention discloses a mouse NK cell in-vitro culture method and application, and belongs to the technical field of cell culture. The method comprises the following two culture steps: firstly, culturing for 1.5-2.5 days by using an activated culture medium containing an MEMS alpha culture medium, 15-25v / v% of FBS, beta-ME and mIL-2 (optional mIL-15 and mIL-12); and culturing for 16.5-17.5 days by using an amplification culture medium containing an MEMS alpha culture medium, FBS, beta-ME and mIL-2, wherein the cell density is controlled to be less than or equal to 2E < 6 > / mL in the whole culture process. According to the culture method provided by the invention, the amplification efficiency of the mNK cells can be remarkably improved, the initial proliferation speed of a multi-factor activation group is higher, the cells are maintained in a normal form, the transduction efficiency is improved, and sufficient high-activity cells are provided for research of the mNK cells.

Cell-binding agents linked to beads are used to attach the beads to cells that are subsequently partitions. Methods, reaction mixtures, and kits as well as other aspects are provided.

This invention is an advanced bioimaging device allowing for the observation and analysis of living cells and intracellular reactions in real-time without the need for staining. It uses a combination of nanophotonic microscopy, nanobiological sensors, artificial intelligence processing, and ultra-sensitive spectroscopy technology to record and analyze intracellular changes without destroying the biological structure. The key feature of this technology is non-invasive and live imaging of cells and tissues without the need to add dyes or external chemicals that can affect the normal behavior of the cell. It offers higher resolution, no chemical interference, and the ability to automatically analyze data compared to traditional imaging methods such as fluorescence and phase contrast. It also includes cell research, drug development, personalized medicine, biotechnology, nanotechnology, and medical diagnostics. This invention is designed to study molecular, cellular, and biological processes in living cells and can simultaneously perform imaging and data analysis.

The invention discloses a method for observing local death of cyanobacteria cells by using a UV device, and relates to the technical field of cell research, and the method comprises the following steps: culturing cyanobacteria cells to a logarithmic phase to obtain a cyanobacteria sample; preparing an observation device; dropwise adding a cyanobacteria sample to the surface of the solidified culture medium, and air-drying and fixing; locally irradiating a sample area below the opening through a UV light source; sealing the sample through a cover glass and a second silicone rubber film sheet, and transferring the marking line to the cover glass; and observing the death process of cyanobacteria cells. Local precise UV stress of cyanobacteria protonema is realized by customizing a shading plate with a local opening, in-situ long-time stable observation is guaranteed by matching agar fixation and a sealed chamber design, and a continuous fluorescence microscopic imaging technology is combined; and the accurate visual tracking of the death time sequence, spatial distribution and death signal transmission process of different cells on the single protonema is realized.

This invention discloses a method for synthesizing 4',7-O-dimethylquercetin and its applications, belonging to the field of pharmaceutical synthesis and application. 4',7-O-dimethylquercetin is chemically synthesized using a partially substituted rutin intermediate as a raw material. Under 365 nm ultraviolet light irradiation, 4',7-O-dimethylquercetin can generate excessive amounts of reactive oxygen species (ROS). In vitro cell studies show that it inhibits cancer cell proliferation under ultraviolet light irradiation, indicating that this compound has good application prospects as a photosensitizer in cancer chemotherapy.

1. The name of the design product: cell in-situ culture and complex impedance detector. 2. The use of the design product: the design product is used for experimental instruments for high-throughput, multi-channel electrophysiological signal acquisition, and cell research in the field of biomedicine. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.