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38 results about "Cell studies" patented technology
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Perfusion bioreactors for culturing cells
ActiveUS20060110822A1Bioreactor/fermenter combinationsBiological substance pretreatmentsPerfusion bioreactorCulture cell
A bioreactor system comprising a multi-well platform including an array of bioreactor units. The bioreactor system comprises a perfusion unit and a fluid source unit fluidly interconnected by a pumping unit. The perfusion unit comprises a multi-well plate including a plurality of main chambers configured to house or contain a cell culture and in each bioreactor unit an independent cell study or experiment may be performed.
Owner:ORGANOVO
Perfusion bioreactors for culturing cells
ActiveUS7767446B2Bioreactor/fermenter combinationsBiological substance pretreatmentsPerfusion bioreactorCulture cell
A bioreactor system comprising a multi-well platform including an array of bioreactor units. The bioreactor system comprises a perfusion unit and a fluid source unit fluidly interconnected by a pumping unit. The perfusion unit comprises a multi-well plate including a plurality of main chambers configured to house or contain a cell culture and in each bioreactor unit an independent cell study or experiment may be performed.
Owner:ORGANOVO
Optical microscopy and its use in the study of cells
InactiveUS6929934B1Minimize damageExposure of light can be limitedBioreactor/fermenter combinationsBiological substance pretreatmentsIon currentLive cell imaging
The present invention pertains to an apparatus for imaging an object, comprising a probe via which an assay component may be delivered; a sensor to detect ion current; and means for controlling the position of the probe relative to the object in response to the ion current. Such apparatus can be used to image live cells, without affecting them, in solution, e.g., using light, wherein the distance between probe and cell is less than the wavelength of light.
Owner:IONSCOPE
Method for preparing hydrogel microparticles based on paper chip technology
InactiveCN102806053AUniform sizeGood size controlColloidal chemistry detailsComputer printingPharmaceutical drug
The invention provides a method for preparing hydrogel microparticles based on a paper chip technology. A paper chip consists of patterns which are sprayed and printed on paper by a wax spray printer, and is immersed in a solution containing calcium ions and alginate to form hemispheric alginate hydrogel microparticles with codes; and the formed hemispheric microparticles can be combined under a microscope to form asymmetrical spherical hydrogel microparticles. The asymmetrical microparticles can be applied to mutual effects among cells, the irritation influence of medicines on the cells and the like, and have an excellent application prospect on the study of the cells, the screening of the medicines and the like. The method is simple, easy to operate and low in cost, and the prepared microparticles have the advantages of uniform sizes, controllable sizes and the like.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Layered co-culture method for intramuscular fat cells and skeletal muscle cells of mammal
InactiveCN103773733AFew stepsSimple technologyArtificial cell constructsSkeletal/connective tissue cellsSerum free mediaMuscle tissue
The invention discloses a layered co-culture method for intramuscular fat cells and skeletal muscle cells of a mammal. The layered co-culture method comprises the steps: after washing and cutting a longissimus tissue on the back of a butchered animal into pieces, digesting the longissimus tissue by using type-I collagenase, and sieving a digested mixed solution; collecting filtrate, centrifuging, respectively collecting mature fat cell sap on the upper layer and muscle cells on the lower layer, respectively adding a proper amount of serum-free medium to dilute and wash, and centrifuging; adding the centrifugate on the upper layer and the muscle cell sap on the lower layer into a culture flask with the capacity of 25cm<2>, and culturing in a culture box with the temperature of 37 DEG C and the CO2 content of 5%. The mature fat cells can float and adhere to a ceiling surface on the upper layer because of small buoyancy, while muscle cells sink to the bottom of the culture flask to grow. The cell culture model can be used for layering and co-culturing the intramuscular fat cells and the muscle cells of the same muscle tissue of an animal in the same culture solution, so that a novel in-vitro cell research model is provided for researching the influence of mature fat cell secreta to muscle cell differentiation or the interaction between the muscle cells and the fat cells.
Owner:NANJING AGRICULTURAL UNIVERSITY
Cell research centrifugal equipment for fixing test tube through negative pressure
The invention relates to the technical field of biological cells, and discloses cell research centrifugal equipment for fixing a test tube through negative pressure. The cell research centrifugal equipment comprises a motor, the top of the motor is rotatably connected with a rotating shaft penetrating through a fixed table, the top of the rotating shaft is fixedly connected with a storage plate, storage cylinders arranged at equal intervals are inserted into the tops of the storage plate, storage grooves are formed in the tops of the storage cylinders, air cavities are formed in the lower portions of the interiors of the storage cylinders, threaded rods are rotatably connected to the inner top walls of the air cavities, pinions are fixedly connected to the bottoms of the threaded rods, andthe threaded rods is sleeved with pistons and ratchet wheels. When the pistons reach the bottom wall of the air cavity, the pistons stop moving downwards, the threaded rods drive the storage cylinders to rotate on the storage plate under the action of the pinions, so that the effect that the centrifugal test tube rotates while revolving around the center of the storage plate is achieved, the movement of the centrifugal test tube is more variable and complex, and the centrifugal effect of biological cells is better.
Owner:广州硕泰科技有限公司
Preparation method and application of nanowire biosensor
ActiveCN111175347ASmall sizeHigh detection sensitivityMaterial nanotechnologyVacuum evaporation coatingDiseaseNanowire
The invention relates to a preparation method and application of a nanowire biosensor, and belongs to the technical field of biological testing. According to the preparation method of the nanowire biosensor, the corresponding single-stranded nucleic acid probe is designed according to a target to be detected, and the target nucleic acid can be detected. Meanwhile, the continuous dynamic detectionof the target nucleic acid can be realized in situ through the nucleic acid melting and in-situ release technology of electric heating so that the concentration change condition of the target nucleicacid molecule is mastered. The nanowire biosensor prepared by the method can be used for conveniently and quickly preparing the required nanowire biosensor and is used for simply and quickly realizingunlinking and in-situ release of a biomarker and can be used for early diagnosis and prognosis of a detected person according to the concentration change of the specific biomarker aiming at the biomarker of a certain disease and can be widely applied to the fields of tumor early screening and prognosis monitoring, single cell research, embryo culture and development in assisted reproduction and the like.
Owner:TSINGHUA UNIV
Purpose of hydrogen gas to preparation of renal fibrosis treatment preparation
InactiveCN107648257AReduced renal fibrosisLessen kidney damageInorganic active ingredientsUrinary disorderPharmaceutical formulationBiopharmacology
The invention belongs to the field of biopharmacology, and relates to a purpose of hydrogen gas to preparation of renal fibrosis treatment medicine, in particular to a purpose of hydrogen gas to preparation of medicine for treating renal fibrosis caused by chronic nephrosis. A built animal model is subjected to biochemical index detection, isolated cell study, western blotting experiments, immunohistochemistry and data analysis after hydrogen gas intervening; the result shows that the hydrogen gas can realize the fibrosis inhibition on the pathological environment induction through an sirt1 path; the harm to the renal function unit in the fibrosis process is reduced through improving the sirt1; and the hydrogen gas can be further used for preparing a medicine preparation for treating renalfibrosis, particularly the renal fibrosis caused by chronic nephrosis.
Owner:FUDAN UNIV +1
Method for extracting anchorage-dependent cells and extracting device for method
PendingCN110724658ASimple structureLow costBioreactor/fermenter combinationsCell dissociation methodsMedicineEngineering
The invention relates to a method for extracting anchorage-dependent cells. The method comprises the following steps of preprocessing polyacrylamide hydrogel, culturing anchorage-dependent cells, integrally excavating the cells and the adhered gel, removing surface gel and the like. According to the method, an anchorage-dependent cell extracting device suitable for the extracting method is also arranged. The extracting method disclosed by the invention is convenient and easy to operate, and the extracting of the anchorage-dependent cells can be completed without tedious steps, so that the possibility of mistaking is reduced; besides, the adopted extracting device is simple in structure and low in cost; and in addition, the extracting method is small in damage to the anchorage-dependent cells, and has important significance to cell research.
Owner:BONE MEDICAL TECH OF SUZHOU CO LTD
Ghrelin o-acyltransferase inhibitors
InactiveUS20170056373A1Robust inhibitionNitrile/isonitrile active ingredientsDiseaseHigh-Throughput Screening Methods
Small molecule ghrelin O-acyltransferase inhibitors found using an assay to detect ghrelin O-acyltransferase activity using an acrylodan-labeled peptide mimic of ghrelin that provides for high-throughput screening for ghrelin O-acyltransferase inhibitors and detection via high performance liquid chromatography. The newly discovered class of synthetic triterpenoids efficiently inhibits ghrelin acylation by GOAT and function as covalent reversible inhibitors of GOAT. In cell studies, the most potent members of this family of compounds efficiently block ghrelin acylation at submicromolar concentrations and offer a foundation for continued development and evaluation of novel hGOAT inhibitors as therapeutics targeting disorders such obesity, type II diabetes, gastroparesis, and Prader-Willi syndrome.
Owner:SYRACUSE UNIVERSITY
SARS-CoV-2 specific polypeptides and application thereof
PendingCN113845577AImprove featuresIncreased sensitivitySsRNA viruses positive-senseViral antigen ingredientsActivation cellsT cell
The invention discloses SARS-CoV-2 specific polypeptides and application thereof, and belongs to the field of immunodetection. The invention provides two specific polypeptides aiming at SARS-CoV-2; a polypeptide-MHC tetramer is prepared from the corresponding polypeptides; the polypeptide-MHC tetramer is used for detecting T cells of SARS-CoV-2 infected convalescent patients; the positive rates are 3.63% and 2.37% respectively and are obviously higher than 0.061% and 0.096% of a negative control group; the affinity of the polypeptide-MHC and TCR on the surfaces of specific T cells is effectively increased; the technology can be used as an effective tool for T cell evaluation, can also be used for separating and cloning the specific T cells, is combined with a single cell sequencing technology to separate specific TCR, and can be used as a T cell activation reagent and the like; and the technology has a relatively high application value in the aspect of researching the T cells of people infected with the SARS-CoV-2 or vaccinated with the SARS-CoV-2.
Owner:STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
G6PD over-expressed ACHN stable cell strain and construction method thereof
InactiveCN104531622AIncrease enzyme activityFermentationVector-based foreign material introductionWestern blotResuscitation
The invention relates to a related technology of medical molecular biology, and in particular relates to a cell research model constructed by adopting a siRNA jamming technology. A G6PD over-expressed ACHN stable cell strain disclosed by the invention is a G6PD over-expressed ACHN stable cell strain. The cell strain is obtained by pressurizing and screening a pBABE-puro-G6PD transfected human renal cell adenocarcinoma cell strain ACHN through puromycin, verifying Real-time PCR and Western blot, carrying out repeated passage and cryopreservation resuscitation. The G6PD expressed quantity of the obtained cell strain is increased by above 40 times. A construction method disclosed by the invention comprises the following steps of: 1, cloning a G6PD gene; 2, constructing a pBABE-puro-G6PD recombinant expressed vector; 3, carrying out transfection on an ACHN cell strain; and 4, screening and identifying an ACHN-G6PD cell strain. Construction of the stable cell strain provides a cell model for researching the relevance between G6PD and renal cell carcinoma and relevance mechanism and also can lay a foundation for generation and development mechanism of renal cell carcinoma, and discovery of a novel drug action target.
Owner:KUNMING MEDICAL UNIVERSITY
Test tube rack for biological cell research and application thereof
InactiveCN111940005AGuaranteed to workEasy to useBioreactor/fermenter combinationsBiological substance pretreatmentsTissue sampleCell organisation
The invention discloses a test tube rack for biological cell research and application thereof, belonging to the technical field of biological cells. The test tube rack comprise a coil, wherein an outer mounting plate is movably connected to the interior of the coil, and a test tube ring is fixedly connected to the inner side of the outer mounting plate. According to the test tube rack for biological cell research and the application of the test tube rack, through the advantage of a self-stop function under the an overspeed condition, the centrifugal process of a test tube can be automaticallystopped through the structure of the test tube rack when the rotating speed of centrifugal equipment exceeds a rated value, so the problem that cell tissue samples are damaged due to overlarge centrifugal force is solved, cell tissue is prevented from being damaged, and cell tissue inactivation caused by stall is avoided. Compared with the prior art, the method has the advantages that the effectiveness of a centrifuged cell tissue sample is improved, the authenticity of experimental data is guaranteed, the credibility of an experimental result is improved, the problem of experimental process delay caused by cell tissue inactivation can be avoided, time and cell tissue resources are saved to a certain extent, and the requirements of biological cell research are better met.
Owner:韦文杰
Preparation method of novel cell additive and novel cell additive product
PendingCN110684715ANo toxicityNo solid precipitationCulture processCell culture mediaDyslipidemiaSodium stearate
The invention provides a preparation method of a novel cell additive and a novel cell additive product, and relates to the field of in vitro cell research for dyslipidemia relevant diseases. The method comprises the steps of adding a stearic acid component solution obtained by high-temperature dissolving to a BSA component solution obtained by room-temperature dissolving, performing shaking for uniform mixing, and after sufficient shaking combination, performing filtering to remove bacteria to obtain the novel cell additive product. A preparation method provided by the invention can solve thetechnical problems that in the prior art, in the preparation process of stearic acid or sodium stearate, solvent toxicity, difficult dissolving, easy separation and the like exist, and has the advantages that the concentration of the stearic acid or the sodium stearate is precise and controllable, products are good in stability, easy to store and the like, and the produced novel cell additive belongs to a ready-to-use product.
Owner:郭永珍
Human umbilical cord-derived mesenchymal stem cells and preparation method thereof
InactiveCN111893092AActivity without lossHigh purityCell dissociation methodsSkeletal/connective tissue cellsWharton's jellyMedicine
The invention discloses a preparation method of human umbilical cord-derived mesenchymal stem cells. According to the preparation method, an umbilical cord of a full-term healthy fetus is adopted to perform aseptic processing, mechanical shearing, exposure of Wharton's jelly, digestion with collagenase, and then separation and culture to prepare the human umbilical cord-derived mesenchymal stem cells, the cell viability does not decrease significantly after cryopreservation and resuscitation, and thereby the human umbilical cord-derived mesenchymal stem cells are an ideal cell source for clinical use. The invention also relates to the mesenchymal stem cells prepared by the method, and the mesenchymal stem cells are confirmed to be mesenchymal stem cells by performing morphology, immunophenotype and in vitro differentiation experiments in accordance with the standards of mesenchymal stem cells formulated by the International Society for Stem Cell Research (ISSCR). The number of cells, cell viability, purity, doubling time, and proliferation ability of the mesenchymal stem cells are significantly higher than those of bone marrow-derived mesenchymal stem cells.
Owner:国大生命科学产业集团(深圳)有限公司
Daphnoretin micelle and preparation method, content detection and application thereof
ActiveCN113499310AGood reproducibilityImprove performancePowder deliveryComponent separationLiver targetingAnti neoplastic
The invention relates to a daphnoretin micelle and a preparation method, content detection and application thereof. The daphnoretin nano-micelle is prepared by selecting a safe and degradable material PEG-PLA, and daphnoretin is prepared into a polymer micelle so as to achieve the purposes of increasing solubility and prolonging in-vivo action time. Meanwhile, the glycyrrhetinic acid coupled PEG-PLA is used as a carrier, and the nano preparation which is loaded with daphnoretin and has the liver cancer targeting property is prepared. Through in-vitro cell research, in-vivo targeting evaluation and preliminary pharmacodynamic research on the two drug-loaded micelles, the liver targeting drug delivery performance and the anti-tumor effect of the daphnoretin nano-micelle drug system are proved, and a theoretical basis is laid for in-vivo liver cancer targeting research of daphnoretin in the future; and a new thought is provided for the development of a new daphnoretin dosage form and a novel liver cancer targeting preparation.
Owner:贵州中医药大学
Chip for open single cell research and preparation method thereof
ActiveCN107828653BEasy to shareEasy to makeBioreactor/fermenter combinationsBiological substance pretreatmentsCell trappingEngineering
Owner:INST OF SEMICONDUCTORS - CHINESE ACAD OF SCI
Composition for treating esophageal cancer and application thereof
ActiveCN111228384BGreat market potentialSteroidsAntineoplastic agentsSeparation technologyCancer research
Owner:HENAN UNIV OF CHINESE MEDICINE
High-flux cell sorting and enriching device and using method thereof
ActiveCN111500417ARaise the liquid levelRelieve pressureBioreactor/fermenter combinationsBiological substance pretreatmentsCell throughputCell cluster
Aiming at the technical defects of low target cell screening flux, large damage, easy loss and pollution of recovered cells and the like in the existing cell research technology, the invention provides a high-flux cell sorting and enriching device and a using method thereof. The device has the following advantages: 1) cell enrichment, recovery, capture and culture are integrated in a closed mode,so the problems of inter-link cell loss and pollution are solved; 2) on a cell separation module, by combining an inclined cell filter membrane and a fluid channel, the target cell flux is kept on thebasis of keeping the cell activity; and 3) a single cell ( / cell cluster) capture and culture observation module solves the problem of cell tracking and positioning detection, and effectively culturesproliferative cells. The device is simple and convenient to operate, and provides equipment technical support for preparation and amplification of rare cell samples and single cell accurate researchin biomedicine and clinical diagnosis research.
Owner:INST OF MICROELECTRONICS CHINESE ACAD OF SCI
Mers-cov specific polypeptide and its application
ActiveCN106397549BImprove featuresIncreased sensitivitySsRNA viruses positive-senseAntibody mimetics/scaffoldsStainingT cell
The invention discloses MERS-CoV specific polypeptides and application thereof, belongs to the field of immunodetection and provides three MERS-CoV specific polypeptides aiming at BABL / c mice. An MHC-tetramer is prepared by using corresponding polypeptides, affinity between polypeptide-MHC and specific T cell surface TCR is improved effectively, and the MHC-tetramer can serve as an effective tool for T cell evaluation. The technology can be used for separating and cloning of specific T cells and performing in-situ dyeing on mouse corresponding tissue slices after MERS-CoV infection or vaccination and has high application value in the studying aspect of T cells of a mouse model after MERS-CoV infection or vaccination.
Owner:STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT +1
Separation method for cell cluster having tumorigenic potential in liver cancer tissue
The invention discloses a separation method for a cell cluster having a tumorigenic potential in liver cancer tissue. By using the method, the cell cluster having the capacities of growth and proliferation in vitro, colony forming and tolerance to anticancer drugs can be separated from a clinical sample quickly, easily and effectively, and the passage cells have certain stability and are the foundation of separating and identifying the source cells having high tumorigenicity. In the condition of studying liver cancer stem cell and micro-environment for liver cancer stem cell growth without enough stable cells that are hard to be obtained in primary tissue, the study of the composition of the cell clusters and the role of the cell clusters in maintaining the continuous increasing of tumor cells, resistance to drugs and tumorigenicity can help researchers to gradually narrow the search area, so as to finally lock the liver cancer stem cell that causing a liver caner and a recurrence after treatment, and combine the study of the micro-environment of the cell to explore a feasible scheme for clinic treatment of great reference value.
Owner:ZHEJIANG UNIV
Method for constructing Alzheimer's disease (AD) cell model, and application of Alzheimer's disease (AD) cell model
PendingCN112980887AGuaranteed longevityGuaranteed stabilityCompound screeningApoptosis detectionChinese hamsterCell
The invention discloses a method for constructing an Alzheimer's disease (AD) cell model, and application of the Alzheimer's disease (AD) cell model. The method comprises the following steps of: constructing a recombinant vector containing a coding gene of APP protein, and integrating the coding gene of the APP protein into a genome of a host cell by using the recombinant vector, so as to construct and form the cell model. According to the invention, CHO (Chinese Hamster Ovary) and HFF (Human Foreskin Fibroblast) cell lines overexpressing APP genes are constructed through a gene technology to serve as the AD cell model; a lentivirus transfection mode is adopted, so that it can be guaranteed that the AD cell model has the durability and stability of AD pathological characteristics; moreover, the AD cell model disclosed by the invention can stably express and secrete A beta, can be used for a long time, has stable and continuous A beta deposition and tau protein phosphorylation characteristics, and can well simulate pathological characteristics of the Alzheimer's disease; and an in-vitro cell research platform possibility is provided for research, development and screening of Alzheimer's disease related drugs and research of mechanisms.
Owner:SHANGHAI UNIV +1
Monomolecular mechanics method for measuring accurate length of human telomere
PendingCN111549098ALow influence of length measurementMicrobiological testing/measurementSequence analysisEnzyme digestionAntiendomysial antibodies
The invention relates to a monomolecular mechanics method for measuring the accurate length of human telomere. The method comprises the following steps of: firstly, extracting genome DNA of a target cell; digesting the genome by using combined enzyme digestion, and reserving telomere DNA; carrying out affinity labeling on the two ends of telomere DNA by adopting digoxin and biotin respectively soas to obtain a telomere DNA structure capable of being used for Monomolecular mechanical measurement; secondly, fixing the telomere structure between streptavidin-coated magnetic beads and the glass surface paved with the anti-digoxin antibody, and carrying out a DNA mechanical tensile experiment on a monomolecular instrument; and finally, using a worm model to perform length conversion between nanometer and basic group pairs on the actually measured telomere length, so as to realize accurate measurement of the telomere length. The invention belongs to the field of precise instrument measurement and analysis and cell molecular biology, and develops a new method for directly measuring the accurate length of telomere for cell research, health screening and clinical drug therapeutic effect evaluation.
Owner:NANKAI UNIV
Human orbit preadipocyte cell line, construction method and application
PendingCN113493771AMicrobiological testing/measurementGenetically modified cellsPrecursor cellSomatic cell
The invention discloses a human orbit preadipocyte cell line, a construction method and application. The cell line is obtained by immortalizing human orbit preadipocytes. The construction method of the cell line comprises the following steps of step 1, separating and culturing human orbit preadipocytes from human orbit adipose tissues; step 2, transfecting human orbit preadipocytes with the vector connected with hTERT gene, so that the cells overexpress hTERT; and step 3, screening out human orbit preadipocytes with negative CD90 expression. The cell line can be used as a cell research model for drug screening.
Owner:SHANGHAI FIRST PEOPLES HOSPITAL
Method for detecting anti-human epidermal growth factor receptor 2 monoclonal antibody-MMAE conjugate neutralizing antibody in human serum
InactiveCN109280687AImprove toleranceHigh neutralizing activityMicrobiological testing/measurementBiological testingAntigenPositive control
A method for detecting an anti-human epidermal growth factor receptor 2 monoclonal antibody-MMAE conjugate neutralizing antibody in human serum includes steps of: 1) with SK-BR-3 cell line being a target cell, researching bioactivity of a medicine; 2) immunizing a machin with the medicine as an antigen and enhancing the immunization with a medicine F(ab)'2 fragment to prepare a positive polyclonalantibody; 3) pretreating a serum sample by using SPE extraction to eliminate serum interference, thus increasing stability of the method and drug tolerance capability; 4) developing a neutralizing antibody detection method on the basis of the medicine activity method, and applying the detection method to detection on the neutralizing antibody in the pretreated serum sample. The method is sourcedfrom cell level-based bioactivity method and can directly reflect the molecular mechanism of action (MoA) of the medicine. By using the SK-BR-3 cell that is more sensitive to medicine action, the method is improved in sensitivity. The positive control antibody is prepared by immunizing a non-human primate animal, so that comparability of the method with an actual sample is better. The serum sampleis detected after the pretreatment, so that the detection results are more stable and reliable.
Owner:上海有临医药科技有限公司
A kind of core-shell nano-silica fluorescent probe and its synthesis method and application
ActiveCN108003862BRapid Analysis SortingGreat application potentialMaterial nanotechnologyMonoazo dyesBiotechnologyMicroorganism
The invention discloses a core-shell nano-silica fluorescent probe, a synthesis method and an application thereof. The core-shell nano-silica fluorescent probe of the present invention is a nanoparticle of a core-shell structure, and the core-shell structure includes an inner fluorescent dye shown in formula (I) and a shell dye shown in formula (II), and the nanoparticle The structure is shown in formula (III); the present invention also discloses a method for using a core-shell nano-silica fluorescent probe to select and breed microorganisms with toxic aromatic hydrocarbon degradation activity. The technology-based microbial breeding research mode can perform rapid and highly sensitive analysis and sorting of single cells more efficiently, intuitively and specifically, and can provide new tools for efficient breeding of functional microorganisms, and can also provide a basis for single-cell research and development of functional microorganisms. The in-depth excavation of uncultivated microorganisms provides technical support, which has great practical significance and application potential for the prevention and control of toxic aromatic hydrocarbon pollutants.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
Ultralow-temperature cell transportation and storage device for stem cell research
InactiveCN114190370AImprove cooling effectAvoid hot and cold swapsLiving organism packagingDead animal preservationCold airThermodynamics
The invention discloses an ultralow temperature cell transportation and storage device for stem cell research, the transportation and storage device comprises a transportation box, a storage box is arranged in the transportation box, a cooling pipe is arranged above the storage box in the transportation box, a control box is arranged below the storage box, the cooling pipe is connected with the storage box through a pipeline, and the control box is connected with the storage box through a pipeline. The control box enables the storage box to be kept in a vertical state. The cooling pipe extracts outside air and heats and sterilizes the air through the hot end generated by the Peltier effect, the cooling pipe cools the hot air through the cold end generated by the Peltier effect again, moisture in the hot air is condensed into low-temperature water, the hot air is cooled into cold air, the cooling pipe conveys the low-temperature water into the storage box, and the storage box stores the low-temperature water. The storage box cools the stem cells through low-temperature water, the cooling pipe injects cold air into the box body of the storage box, the cold air cools the box body, and the situation that the cold-heat exchange between the box body and the low-temperature water affects the effect of refrigerating the stem cells is prevented.
Owner:广州维特利科技有限公司
Application of Raw264.7 cell line capable of stably expressing different human ApoE genotypes and preparation method of Raw264.7 cell line
The invention discloses an application of a Raw264.7 cell line capable of stably expressing different human ApoE genotypes in researching the influence of the ApoE genotypes on inflammation immunity, and a preparation method of the stably transfected cell line. The preparation method comprises the following steps: transfecting different human ApoE genotype plasmids ApoE2, ApoE3 and ApoE4 with EGFP labels and a control plasmid N1 into a Raw264.7 cell by using a transfection reagent; after a positive screening agent G418 is added for selective culture, a stably transfected cell strain is obtained through multiple times of sorting in a flow cytometry single cell sorting mode, and ApoE genotype and expression identification is carried out; the stably transfected cell is used for researching the influence of the ApoE genotype on NLRP3 inflammasome activation, endogenous melatonin synthesis, mitochondrial energy metabolism, pathogen removal and the like. The cell strain provides a good tool for further research on biological characteristics of different ApoE genotypes in the field of inflammation immunity and research and development of drugs.
Owner:THE FIRST AFFILIATED HOSPITAL OF ANHUI MEDICAL UNIV
A light-sensitive targeted anti-tumor prodrug that responds to nitroreductase to kill tumor cells and its preparation method and application
The invention discloses designs and synthesizes a nitroreductase and light stimulation drug and fluorescence dual release system by taking nitroreductase as a target molecule for drug release based on design and application of nitroreductase and light stimulation drug and fluorescence dual release system. A nitroreductase-light stimulated drug and fluorescence double-release system is designed and synthesized by taking nitroreductase as a target molecule released by the drug as well as boric acid ester as a response group. The determination of the fluorescence of the predrug (CM-3) finds that the predrug (CM-3) can well release fluorescence in response to nitroreductase; meanwhile, compared with other light-sensitive drugs, the predrug has relatively good stability and targeting property; and a research for determining the anti-tumor activity of the predrug by virtue of an MMT method finds that the compound (CM-3) has the anti-tumor activity higher than that of chlorambucil, namely that the targeting property of the compound (CM-3) is higher than that of chlorambucil. According to the predrug, an effective research tool is provided for drug release in cell researches.
Owner:ZHEJIANG UNIV OF TECH
Cell scraper device with scraper blade convenient to replace
PendingCN114164077ASimple structureEasy to operateBioreactor/fermenter combinationsBiological substance pretreatmentsPetri dishStructural engineering
The invention relates to the field of equipment for cell research, and discloses a cell scraper device with a scraper blade convenient to replace, which comprises the scraper blade, a baffle plate, a connecting cylinder, a handle, a connecting handle, a push plate, a sliding block I, a spring, an elliptical plate, a rotating rod I, a rotating rod II, a limiting piece, a driving rod, a driving groove, a rack I, a rack II, a connecting rod I, a strip-shaped through hole I and a round block I. The cell scraping device is simple in structure, cells can be scraped, cell solution scraped from a culture dish can be sucked, the scraping plate and the gun head can be replaced, a pipette can be replaced, and multiple purposes are achieved; the operation mode is simple and convenient, the manufacturing cost is low, only the scraper blade needs to be replaced, resources are saved, and the use cost is also low.
Owner:THE AFFILIATED HOSPITAL OF TRADITIONAL CHINESE MEDICAL TO SOUTHWEST MEDICAL UNIV
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