38 results about "Cell studies" patented technology

A bioreactor system comprising a multi-well platform including an array of bioreactor units. The bioreactor system comprises a perfusion unit and a fluid source unit fluidly interconnected by a pumping unit. The perfusion unit comprises a multi-well plate including a plurality of main chambers configured to house or contain a cell culture and in each bioreactor unit an independent cell study or experiment may be performed.

The invention provides a method for preparing hydrogel microparticles based on a paper chip technology. A paper chip consists of patterns which are sprayed and printed on paper by a wax spray printer, and is immersed in a solution containing calcium ions and alginate to form hemispheric alginate hydrogel microparticles with codes; and the formed hemispheric microparticles can be combined under a microscope to form asymmetrical spherical hydrogel microparticles. The asymmetrical microparticles can be applied to mutual effects among cells, the irritation influence of medicines on the cells and the like, and have an excellent application prospect on the study of the cells, the screening of the medicines and the like. The method is simple, easy to operate and low in cost, and the prepared microparticles have the advantages of uniform sizes, controllable sizes and the like.

An open single-cell research chip and its preparation method, the chip includes a packaging structure surrounded by a substrate, a retaining wall and a transparent cover, the substrate has a microhole array structure inside the packaging structure, the A number of microwells in the microwell array structure contain single cells respectively, and droplets of reagents for single cell research are respectively added to the top of each microwell by 3D printing, and the remaining space in the packaging structure is filled with sealing oil, further providing The manufacturing method of the chip. The invention perfectly combines 3D printing technology with single-cell microwell arrays, efficiently and quickly realizes the research on the single-cell level, establishes a single-cell research system with simple structure, wide application range and perfect functions, and realizes high occupation High-rate single-cell capture, multi-step liquid treatment of single cells with diversity, accuracy, and open structure can be applied to a variety of research systems.

The invention discloses a composition for treating esophageal cancer and its application. The technical scheme is that the invention uses a cell model, is guided by activity tracking, and adopts modern extraction and separation technologies such as alcohol extraction, chromatography, and high performance liquid chromatography. Active monomers were extracted from four traditional Chinese medicines with anti-cancer activity: Pangolinus, Zhuyazao, Tubeimu and Chonglou, and the chemical spectrogram method was used to speculate on the molecular composition and determine the molecular structure of each monomer, which was determined by the baseline equal ratio increase and decrease method The dosage ratio of the monomers is combined into a composition for treating esophageal cancer, and then the active monomers and the composition are used to act on various esophageal cancer cells to study their mechanism of action on esophageal cancer. The four active monomers and their compositions provided by the invention are all derived from the extraction of natural medicines, are pure traditional Chinese medicine preparations, have the functions of preventing and treating esophageal cancer, and have great market potential and broad market application prospects.

The invention discloses MERS-CoV specific polypeptides and application thereof, belongs to the field of immunodetection and provides three MERS-CoV specific polypeptides aiming at BABL / c mice. An MHC-tetramer is prepared by using corresponding polypeptides, affinity between polypeptide-MHC and specific T cell surface TCR is improved effectively, and the MHC-tetramer can serve as an effective tool for T cell evaluation. The technology can be used for separating and cloning of specific T cells and performing in-situ dyeing on mouse corresponding tissue slices after MERS-CoV infection or vaccination and has high application value in the studying aspect of T cells of a mouse model after MERS-CoV infection or vaccination.

The invention discloses a separation method for a cell cluster having a tumorigenic potential in liver cancer tissue. By using the method, the cell cluster having the capacities of growth and proliferation in vitro, colony forming and tolerance to anticancer drugs can be separated from a clinical sample quickly, easily and effectively, and the passage cells have certain stability and are the foundation of separating and identifying the source cells having high tumorigenicity. In the condition of studying liver cancer stem cell and micro-environment for liver cancer stem cell growth without enough stable cells that are hard to be obtained in primary tissue, the study of the composition of the cell clusters and the role of the cell clusters in maintaining the continuous increasing of tumor cells, resistance to drugs and tumorigenicity can help researchers to gradually narrow the search area, so as to finally lock the liver cancer stem cell that causing a liver caner and a recurrence after treatment, and combine the study of the micro-environment of the cell to explore a feasible scheme for clinic treatment of great reference value.

The invention discloses a core-shell nano-silica fluorescent probe, a synthesis method and an application thereof. The core-shell nano-silica fluorescent probe of the present invention is a nanoparticle of a core-shell structure, and the core-shell structure includes an inner fluorescent dye shown in formula (I) and a shell dye shown in formula (II), and the nanoparticle The structure is shown in formula (III); the present invention also discloses a method for using a core-shell nano-silica fluorescent probe to select and breed microorganisms with toxic aromatic hydrocarbon degradation activity. The technology-based microbial breeding research mode can perform rapid and highly sensitive analysis and sorting of single cells more efficiently, intuitively and specifically, and can provide new tools for efficient breeding of functional microorganisms, and can also provide a basis for single-cell research and development of functional microorganisms. The in-depth excavation of uncultivated microorganisms provides technical support, which has great practical significance and application potential for the prevention and control of toxic aromatic hydrocarbon pollutants.

The invention discloses designs and synthesizes a nitroreductase and light stimulation drug and fluorescence dual release system by taking nitroreductase as a target molecule for drug release based on design and application of nitroreductase and light stimulation drug and fluorescence dual release system. A nitroreductase-light stimulated drug and fluorescence double-release system is designed and synthesized by taking nitroreductase as a target molecule released by the drug as well as boric acid ester as a response group. The determination of the fluorescence of the predrug (CM-3) finds that the predrug (CM-3) can well release fluorescence in response to nitroreductase; meanwhile, compared with other light-sensitive drugs, the predrug has relatively good stability and targeting property; and a research for determining the anti-tumor activity of the predrug by virtue of an MMT method finds that the compound (CM-3) has the anti-tumor activity higher than that of chlorambucil, namely that the targeting property of the compound (CM-3) is higher than that of chlorambucil. According to the predrug, an effective research tool is provided for drug release in cell researches.