Micro fluidic chip device for combined detection of multiple typical tumor marker of female

Abstract

The invention relates to a microfluidic chip device for combined detection of various typical female tumor markers, which belongs to the field of analysis and testing. Using PDMS or polydimethylsiloxane to make the substrate of the microfluidic chip for the combined detection of eight typical tumor markers suitable for women has advantages in price and technology, but there are also difficulties; this case aims to overcome this difficulty . The main point of this case is that the substrate is selected from PDMS with an original ecological surface, and the link-type electromagnetic fixture equipped with a micro-ultrasonic transducer is socketed and positioned in the vicinity of the sample liquid flow terminal of the microfluidic chip. Ultrasound is used to reduce the interfacial tension, which greatly increases the interfacial compatibility between the solid and liquid phases. At the same time, the strong absorption ability of PDMS to ultrasonic waves is used to achieve a rapid decrease in the intensity of ultrasonic waves within a short distance, thereby forming an interface at both ends of the chip. The difference in tension thereby facilitates the flow of the sample fluid toward the terminal along the originally hydrophobic capillary channel. The device eliminates the need for a micropump.

Description

technical field [0001] The invention relates to a microfluidic chip device for combined detection of various typical female tumor markers, which belongs to the field of analysis and testing. Background technique [0002] Tumor markers (tumor markers, TM) refer to a class of substances produced by tumor cells themselves or produced by the body's response to tumor cells during the occurrence and proliferation of tumors, reflecting the existence and growth of tumors, including Proteins, hormones, enzymes (isoenzymes) and oncogene products, etc. Testing the tumor markers in the patient's blood or body fluids can detect tumors early in the tumor screening, observe the curative effect of tumor treatment and judge the prognosis of patients. Currently, the tumor markers commonly used clinically are: (1) alpha-fetoprotein (AFP) is a marker for primary liver cancer, testicular cancer, ovarian cancer and other tumors; (2) carcinoembryonic antigen (CEA) is a marker for digestive system...

AI Technical Summary

Problems solved by technology

[0008] But it's not that simple

[0009] First, this polydimethylsiloxane material, the material referred to by the acronym PDMS, is itself a strongly hydrophobic material. Microchannels are built on this material. If the microchannels are not targeted The modification operation of the surface of the channel, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the micro channel in the structure occupies most of the inner surface of the liquid flow channel, then the PDMS micro channel The strong hydrophobic characteristic of the inner surface of the channel is the decisive factor, which will make it very difficult for the polar liquid flow similar to the aqueous solution to pass through, and its flow resistance is so large that even ordinary micropumps are difficult to push. Of course, If the cover sheet also chooses to use the PDMS material, then the problem is basically the same, with little difference; therefore, in the prior art, it is necessary to modify and modify the inner surface of the microchannel on the PDMS material; then , is this modification operation for the inner surface of the PDMS microchannel very troublesome? That's not the problem. What constitutes a serious technical problem i

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