A kind of method that prepares polypolic acid C from poria cocos

A technology of porcine acid and polyporus, applied in the directions of steroids, organic chemistry, etc., can solve the problems of inability to meet large-scale preparation, generally low yield, weak targeting, etc., and achieves low cost, high purity, and low cost of chromatography solvent. The effect of low environmental pollution

Active Publication Date: 2020-05-08
JIANGSU KANION PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing studies have shown that the pyripolic acid C isolated from Poria cocos has anti-tumor and immune-enhancing effects. Most of the existing methods for isolating and purifying pyripolic acid C use traditional atmospheric silica gel chromatography, and the general yield is low. Poor purity, weak targeting, unable to meet the needs of mass production

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  • A kind of method that prepares polypolic acid C from poria cocos
  • A kind of method that prepares polypolic acid C from poria cocos
  • A kind of method that prepares polypolic acid C from poria cocos

Examples

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Effect test

Embodiment 1

[0034] A method for preparing polyporic acid C from Poria cocos, specifically comprising the following steps:

[0035] (1) Poria cocos piece 120kg is crushed into powder, adds the 85% ethanol of Poria cocos powder 15 times weight volume (being that the weight volume ratio of Poria cocos powder and 85% ethanol is 1kg:15L), reflux extraction 3 times, each 1 hour, get the extract;

[0036] (2) Combine the extracts, filter, and concentrate the obtained filtrate under reduced pressure to obtain a concentrated solution with a specific gravity of 1.2 at 60° C.;

[0037] (3) The concentrated solution was extracted 3 times with equal volumes of ethyl acetate, and the ethyl acetate extraction layer was concentrated under reduced pressure to obtain a thick extract of the ethyl acetate part;

[0038] (4) Add 1.5-2 times the weight of 60-80 mesh silica gel to the thick extract of the ethyl acetate part to mix the sample to obtain the sample silica gel A;

[0039] (5) sample silica gel A ...

Embodiment 2

[0047] A method for preparing polyporic acid C from Poria cocos, specifically comprising the following steps:

[0048] (1) 150 kg of Poria cocos block is crushed into powder, and 70% ethanol of 20 times the weight volume of Poria cocos powder is added, and reflux extraction is performed 4 times, each time for 2 hours, to obtain an extract;

[0049] (2) Combine the extracts, filter, and concentrate the obtained filtrate under reduced pressure to a concentrated solution with a specific gravity of 1.1 at 60°C;

[0050] (3) The concentrated solution is extracted 4 times with equal volumes of ethyl acetate, and the ethyl acetate extraction layer is concentrated under reduced pressure to obtain a thick extract of the ethyl acetate part;

[0051] (4) Add 1.5-2 times 60-80 mesh silica gel to the thick extract of the ethyl acetate part and mix the sample to obtain the sample silica gel A;

[0052] (5) sample silica gel A adopts vacuum liquid chromatography to carry out rough separatio...

Embodiment 3

[0060] A method for preparing polyporic acid C from Poria cocos, specifically comprising the following steps:

[0061] (1) 200 kg of Poria cocos block is crushed into powder, and 95% ethanol of 10 times the weight volume of Poria cocos powder is added, and reflux extraction is performed 5 times, each time for 3 hours, to obtain an extract;

[0062] (2) Combine the extracts, filter, and concentrate the obtained filtrate under reduced pressure to a concentrated solution with a specific gravity of 1.3 at 60°C;

[0063] (3) The concentrated solution is extracted 5 times with equal volumes of ethyl acetate, and the ethyl acetate extraction layer is concentrated under reduced pressure to obtain a thick extract of the ethyl acetate part;

[0064] (4) Add 1.5-2 times 60-80 mesh silica gel to the thick extract of the ethyl acetate part and mix the sample to obtain the sample silica gel A;

[0065] (5) sample silica gel A adopts vacuum liquid chromatography to carry out rough separatio...

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Abstract

The invention discloses a method for preparing polyporenic acid C from Poria cocos. The method comprises the following steps: with Poria cocos as a raw material, carrying out crushing, extraction, filtration, concentration and extraction, subjecting an extract to coarse separation through vacuum liquid chromatography so as to prepare a crude polyporenic acid C product, subjecting the crude polyporenic acid C product to separation and purification through a dynamic axial compression chromatography technology so as to prepare a pure polyporenic acid C product, and carrying out recrystallizationso as to obtain the polyporenic acid C. Compared with a conventional processing method, the method provided by the invention has the advantages of high work efficiency, low cost of a solvent for coarse extraction, high separation precision for a filling material, reusability, low cost of a solvent for chromatography, relatively-low pollution to the environment, high purity of the polyporenic acidC, etc., and is applicable to industrial preparation.

Description

technical field [0001] The invention belongs to the field of modernization of traditional Chinese medicines, and in particular relates to a method for preparing pigolic acid C from poria cocos. Background technique [0002] Poria cocos, also known as Yuling, Fuling, Wanlinggui, and Poria cocos, is the sclerotia of the fungus Poriacocos (Schw.) Wolf of the family Polybacteriaceae. It is used for edema, oliguria, dizziness and palpitation due to phlegm and retention, deficiency of the spleen, lack of food, loose stool and diarrhea, restlessness, palpitation and insomnia. [0003] Modern pharmacological studies have shown that Poria cocos has the functions of enhancing immunity, delaying aging, anti-tumor, anti-virus, and anti-inflammation. At present, a variety of chemical components have been isolated from Poria cocos, mainly including polysaccharides and terpenoids, in addition to steroids, fatty acids, proteins, adenine, amino acids and various inorganic elements. Existin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00
CPCC07J9/005
Inventor 萧伟李石平宋亚玲谢雪温建辉赵祎武杨林勇黄文哲王振中
Owner JIANGSU KANION PHARMA CO LTD
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