Alteromonas, biological flocculant prepared by utilizing alteromonas and preparation method

An Alternomonas, biological flocculant technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problem of poor targeting of microbial flocculants, affecting the development of microbial flocculants, and single measurement methods, etc. problems, to achieve the ideal flocculation effect, strengthen the neutralization effect, and achieve the effect of less dosage

Inactive Publication Date: 2018-02-23
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research level of microbial flocculants in China is low, its production cost is high, the measurement method is singl

Method used

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  • Alteromonas, biological flocculant prepared by utilizing alteromonas and preparation method
  • Alteromonas, biological flocculant prepared by utilizing alteromonas and preparation method
  • Alteromonas, biological flocculant prepared by utilizing alteromonas and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Isolation and cultivation of strains

[0029] Sampling: Sampling 500mL of activated sludge from the sewage plant, after 7 days of acclimation with oxygen, let it stand still for 30 minutes, and take its supernatant.

[0030] Use a sterile pipette to draw 0.1mL water sample from the supernatant water sample, insert it on the broth solid medium, spread it on a flat plate, and incubate it in an incubator at 37°C for 2 days.

[0031] Select a flat plate with uniform distribution of bacteria species, observe the colonies with different shapes of single colonies, and then pick individual colonies and transfer them to broth slant medium, and carry out purification and culture in an incubator at 37°C for 18 hours.

[0032] Inoculate the isolated strain into the fermentation medium and cultivate it at 37°C and 220rpm / min for 18 hours. The composition of the fermentation medium is: peptone 6g / L, beef extract 4g / L, sodium chloride 3g / L, and the balance is water , the pH is 7.5...

Embodiment 2

[0042] A method for preparing a microbial flocculant, comprising the following steps: inoculating Alteromonas (the screening method for Alteromonas is the same as that in Example 1) into the fermentation medium, cultivating on a shaking table, the shaking table speed is 220rpm / min, The culture temperature is 37° C., and after 16 hours of culture, the fermentation liquid is directly centrifuged to obtain flocculent extracellular products, namely the biological flocculant. The microbial flocculant produced by the Alteromonas is a complex extracellular product of the polysaccharide and protein of the bacteria, which can be directly observed with the naked eye. It is white flocculent and settles to the bottom of the liquid. agent.

[0043] The composition of the fermentation medium is: peptone 5g / L, beef extract 5g / L, sodium chloride 2g / L, the balance is water, and the pH is 7.0.

Embodiment 3

[0045] A method for preparing a microbial flocculant, comprising the following steps: inoculating Alteromonas (the screening method for Alteromonas is the same as that in Example 1) into the fermentation medium, cultivating on a shaking table, the shaking table speed is 220rpm / min, The culture temperature is 37° C. After 20 hours of culture, the fermentation broth is directly centrifuged to obtain flocculent extracellular products, namely the biological flocculant. The microbial flocculant produced by the Alteromonas is a complex extracellular product of the polysaccharide and protein of the bacteria, which can be directly observed with the naked eye. It is white flocculent and settles to the bottom of the liquid. agent.

[0046] The composition of the fermentation medium is: peptone 10g / L, beef extract 3g / L, sodium chloride 4g / L, the balance is water, and the pH is 8.0.

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Abstract

The invention relates to an alteromonas, a biological flocculant prepared by utilizing the alteromonas and a preparation method. The alteromonas is preserved on July 25th, 2017 with the preservation number of CGMCC No.14457. Fermentation products of the alteromonas are directly centrifuged to obtain a floccular extracellular product, namely the biological flocculant, and the biological flocculantis mixed with a solution containing cupric ions, so that a compound biological flocculant is obtained. The alteromonas screened out produces a microorganism flocculant through fermentation; and the microorganism flocculant is easy to separate and extract, the preparation method is simple, workload of biological downstream engineering can be effectively alleviated, cost of the microorganism flocculant is reduced, and production efficiency of the microorganism flocculant is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and relates to an alteromonas bacterium, a biological flocculant prepared by using the bacterium and a preparation method. Background technique [0002] In the current society, the population is highly dense, and my country's water resources are becoming more and more scarce. With the rapid development of industrial technology, the water quality has also declined sharply, and water pollution is serious. Wastewater treatment has always been a hot research direction. These include biochemical methods, adsorption, ion exchange, chemical oxidation and other methods. Flocculation technology is one of the more common methods with lower cost and higher treatment efficiency. Its main function is that the flocculant can combine with large particles in water, as well as pigments, heavy metal ions and other pollutants, and then form larger flocs, which settle to the bottom of the water, thereby pu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C02F1/56C02F1/52C12R1/01
CPCC02F1/5236C02F1/5263C02F1/56C12P1/04C12N1/205C12R2001/01
Inventor 乔长晟李娜李雪李洪张卫
Owner 天津北洋百川生物技术有限公司
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