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Tissue culture method for cibotium barometz spores

A golden retriever and spore technology, applied in the field of golden retriever ridge spore tissue culture and breeding, can solve the problems of low spore artificial environment reproduction coefficient, unsuitable for large-scale breeding, increase the demand for dog ridge, etc., and achieves protection of fern resources. , reducing the infection of viruses or other diseases, the effect of strong vitality

Inactive Publication Date: 2018-02-27
傅明尧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the variety of Chinese patent medicines containing dog spines in domestic pharmaceutical companies, the demand for dog spines has increased, leading to crazy digging among the people, and wild resources have become increasingly scarce. In addition, the breeding of artificially planted dog spines is complicated. The cost of stem section propagation is high, the cycle is long, and it is not suitable for promotion; while the reproduction coefficient of spore artificial environment is low; it is not suitable for large-scale breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of culture method of golden retriever dog ridge spore, comprises the steps:

[0020] (1) Collection of spores: pick the pinnae with sporangia group on the wild golden retriever plant, put it in a clean bag and dry it in the shade naturally, remove impurities after the sporangia fall off naturally, collect the sporangia, and use 0.1% mercuric chloride solution to act After 5 minutes, rinse with sterile water 5 times, dry and collect in a sterile bag;

[0021] (2) Spore pretreatment: the aseptic bag containing the sporangia obtained in step (1) is placed at 2-6° C. for 360 days, and the sporangia are broken to collect the spores, and the spores are disinfected with 75% alcohol for 30 seconds and then centrifuged , rinsed 3 times with sterile water, then sterilized the spores with 5% sodium hypochlorite and centrifuged for 4 minutes, then rinsed 3 times with sterile water; collected the sterilized spores and added sterile saline to make a spore suspension;

[0022]...

Embodiment 2

[0025] A kind of culture method of golden retriever dog ridge spore, comprises the steps:

[0026] (1) Collection of spores: pick the pinnae with sporangia group on the wild golden retriever plant, put it in a clean bag and dry it in the shade naturally, remove impurities after the sporangia fall off naturally, collect the sporangia, and use 0.1% mercuric chloride solution to act After 4 minutes, rinse with sterile water 3 times, dry and collect in a sterile bag;

[0027] (2) spore pretreatment: the aseptic bag containing the sporangia that step (1) obtains is placed in 4 ℃ of preservations for 340 days, breaks open the sporangia and collects the spores, and centrifuges after 30 seconds to the spore disinfection with 70% alcohol, without Rinse with bacterial water for 3 times, then sterilize the spores with 2% sodium hypochlorite and centrifuge for 10 minutes, then rinse with sterile water for 3 times; collect the sterilized spores and add sterile saline to make spore suspensi...

Embodiment 3

[0031] A kind of culture method of golden retriever dog ridge spore, comprises the steps:

[0032] (1) Collection of spores: pick the pinnae with sporangia group on the wild golden retriever plant, put it in a clean bag and dry it in the shade naturally, remove impurities after the sporangia fall off naturally, collect the sporangia, and use 0.1% mercuric chloride solution to act After 6 minutes, rinse with sterile water 5 times, dry and collect in a sterile bag;

[0033] (2) spore pretreatment: the aseptic bag containing the sporangia that step (1) obtains is placed in 6 ℃ of preservations for 380 days, breaks open the sporangia and collects the spores, and centrifuges after 20 seconds to the spore disinfection with 75% alcohol, without Rinse with sterile water for 3 times, then sterilize the spores with 3% sodium hypochlorite and centrifuge for 7 minutes, then rinse with sterile water for 3 times; collect the sterilized spores and add sterile saline to make spore suspension;...

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Abstract

The invention discloses a method for tissue culture of the spores of the golden retriever dog. The pinnae with sporangia clusters growing on the wild golden retriever plant are picked, the sporangia are collected, disinfected with 0.1% mercuric chloride solution and rinsed, dried and collected in Then store the aseptic bag at 2-6°C for 340-380 days, break open the sporangia to collect spores, disinfect and rinse with 70%-75% alcohol, then sterilize with 2-5% sodium hypochlorite and centrifuge and rinse, and then make a spore suspension; apply the spore suspension to inoculate the spore germination medium for germination culture, and when the prothallus grows, transfer to the sporophyte medium for gametophyte transformation and culture; when the gametophyte grows 3 After the leaves are removed, they are transferred to nutrient holes for hardening and cultivation, and finally transplanted in the field. The method for cultivating the spores of the golden retriever dog of the invention has the advantages of simple operation process, orderly growth of seedlings, strong plant vitality and high survival rate, and is suitable for popularization and use.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for tissue culture and breeding of golden retriever spores. Background technique [0002] Cibotium barometz (L.) J.Sm. is a fern of the clamshell fern family. It is recorded in the "Dictionary of Traditional Chinese Medicine" that Cibotium barometz is born on the side of the mountain foot, or in the acidic soil in the shade of the forest. Distributed in the southwest, south, southeast of my country, Henan, Hubei and other places, mainly produced in Sichuan, Fujian, and Zhejiang. In addition, it is also produced in Guangxi, Guangdong, Guizhou, Jiangxi, Hubei and other places. [0003] The rhizome of Golden Retriever Dog’s Ridge, which is dried and depilated, is the traditional Chinese medicine Dog’s Ridge, which was first recorded in "Shen Nong’s Herbal Classic", and has been recorded in all previous editions of "Chinese Pharmacopoeia". , for the treatment of ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 傅明尧
Owner 傅明尧
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